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Id Subject Object Predicate Lexical cue
T1 0-130 Sentence denotes Molecular cloning and expression of a third member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family.
T2 131-360 Sentence denotes N-Deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin initiate a series of chemical modifications that ultimately lead to oligosaccharide sequences with specific ligand binding properties.
T3 361-491 Sentence denotes These reactions are catalyzed by GlcNAc N-deacetylase/N-sulfotransferase (NDST), a monomeric enzyme with two catalytic activities.
T4 492-617 Sentence denotes Two genes encoding NDST isozymes have been described, one from rat liver (NDST1) and another from murine mastocytoma (NDST2).
T5 618-767 Sentence denotes Both isozymes are expressed in tissues in varying amounts, but their relative contribution to heparan sulfate formation in any one tissue is unknown.
T6 768-856 Sentence denotes We now report the identification of a third member of the NDST family, designated NDST3.
T7 857-995 Sentence denotes A full-length cDNA clone (3.2 kilobase pairs) encoding a 873-amino acid protein was obtained from a human fetal/infant brain cDNA library.
T8 996-1092 Sentence denotes Human NDST3 (hNDST3) has a nucleotide sequence homologous but not identical to hNDST1 and NDST2.
T9 1093-1205 Sentence denotes The deduced amino acid sequence shows 70% and 65% amino acid identity to that of hNDST1 and NDST2, respectively.
T10 1206-1344 Sentence denotes A soluble chimera of hNDST3 and protein A exhibited both N-deacetylase and N-sulfotransferase activity, confirming its enzymatic identity.
T11 1345-1451 Sentence denotes Northern blot analysis of human fetal brain poly(A)+ RNA showed a single transcript of 6.4 kilobase pairs.
T12 1452-1629 Sentence denotes Reverse transcription polymerase chain reaction analysis revealed more restricted tissue expression of hNDST3 than hNDST1 and NDST2, and high levels in brain, liver, and kidney.
T13 1630-1724 Sentence denotes Analysis of Chinese hamster ovary cells revealed expression of NDST1 and NDST2, but not NDST3.
T14 1725-1861 Sentence denotes In a Chinese hamster ovary cell mutant exhibiting reduced N-sulfotransferase activity and reduced sulfation of heparan sulfate (Bame, K.
T15 1862-1878 Sentence denotes J., and Esko, J.
T16 1879-1891 Sentence denotes D. (1989) J.
T17 1892-1897 Sentence denotes Biol.
T18 1898-1903 Sentence denotes Chem.
T19 1904-2066 Sentence denotes 264, 8059-8065), expression of NDST1 was greatly reduced, but NDST2 was expressed normally, suggesting that both enzymes are involved in heparan sulfate assembly.
T20 2067-2201 Sentence denotes The discovery of multiple NDST isozymes suggests that the assembly of heparan sulfate is much complicated than previously appreciated.