| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-163 |
Sentence |
denotes |
A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
| T1 |
0-163 |
Sentence |
denotes |
A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
| T1 |
0-163 |
Sentence |
denotes |
A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
| TextSentencer_T2 |
164-346 |
Sentence |
denotes |
A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
| T2 |
164-346 |
Sentence |
denotes |
A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
| T2 |
164-346 |
Sentence |
denotes |
A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
| TextSentencer_T3 |
347-465 |
Sentence |
denotes |
The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
| T3 |
347-465 |
Sentence |
denotes |
The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
| T3 |
347-465 |
Sentence |
denotes |
The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
| TextSentencer_T4 |
466-627 |
Sentence |
denotes |
The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
| T4 |
466-627 |
Sentence |
denotes |
The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
| T4 |
466-627 |
Sentence |
denotes |
The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
| TextSentencer_T5 |
628-740 |
Sentence |
denotes |
The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
| T5 |
628-740 |
Sentence |
denotes |
The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
| T5 |
628-740 |
Sentence |
denotes |
The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
| TextSentencer_T6 |
741-895 |
Sentence |
denotes |
The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
| T6 |
741-895 |
Sentence |
denotes |
The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
| T6 |
741-895 |
Sentence |
denotes |
The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
| TextSentencer_T7 |
896-1104 |
Sentence |
denotes |
The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
| T7 |
896-1104 |
Sentence |
denotes |
The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
| T7 |
896-1104 |
Sentence |
denotes |
The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
| TextSentencer_T8 |
1105-1180 |
Sentence |
denotes |
XEG hydrolyzes its substrates with retention of the anomeric configuration. |
| T8 |
1105-1180 |
Sentence |
denotes |
XEG hydrolyzes its substrates with retention of the anomeric configuration. |
| T8 |
1105-1180 |
Sentence |
denotes |
XEG hydrolyzes its substrates with retention of the anomeric configuration. |
| TextSentencer_T9 |
1181-1288 |
Sentence |
denotes |
The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
| T9 |
1181-1288 |
Sentence |
denotes |
The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
| T9 |
1181-1288 |
Sentence |
denotes |
The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
| TextSentencer_T10 |
1289-1391 |
Sentence |
denotes |
The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
| T10 |
1289-1391 |
Sentence |
denotes |
The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
| T10 |
1289-1391 |
Sentence |
denotes |
The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
| TextSentencer_T11 |
1392-1586 |
Sentence |
denotes |
It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
| T11 |
1392-1586 |
Sentence |
denotes |
It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
| T11 |
1392-1586 |
Sentence |
denotes |
It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |