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PubMed:9870354 JSONTXT 43 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-134 Sentence denotes Purification and characterization of GDP-L-Fuc: N-acetyl beta-D-glucosaminide alpha1-->6fucosyltransferase from human blood platelets.
T1 0-134 Sentence denotes Purification and characterization of GDP-L-Fuc: N-acetyl beta-D-glucosaminide alpha1-->6fucosyltransferase from human blood platelets.
TextSentencer_T2 135-304 Sentence denotes c-6-L-Fucosyltransferase (alpha1,6FucT; EC 2.4.1.68) from human platelets, the enzyme that is released into serum during coagulation of blood, was purified 100,000-fold.
T2 135-304 Sentence denotes c-6-L-Fucosyltransferase (alpha1,6FucT; EC 2.4.1.68) from human platelets, the enzyme that is released into serum during coagulation of blood, was purified 100,000-fold.
TextSentencer_T3 305-523 Sentence denotes The purification required three sequential chromatographic steps: chromatofocusing, affinity column chromatography on GnGn-Gp(asialo-aglacto-transferrin glycopeptide)-CH-Sepharose, and gel filtration of Sephadex G-200.
T3 305-523 Sentence denotes The purification required three sequential chromatographic steps: chromatofocusing, affinity column chromatography on GnGn-Gp(asialo-aglacto-transferrin glycopeptide)-CH-Sepharose, and gel filtration of Sephadex G-200.
TextSentencer_T4 524-795 Sentence denotes The final preparation contained a protein that migrated as a single discrete band Mr of 58,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions, and as a single enzymatically active peak Mr of 58,000 in gel filtration.
T4 524-795 Sentence denotes The final preparation contained a protein that migrated as a single discrete band Mr of 58,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions, and as a single enzymatically active peak Mr of 58,000 in gel filtration.
TextSentencer_T5 796-998 Sentence denotes Although the purified enzyme utilized the biantennary GnGn-Gp as substrate, it was twice as active with the triantennary oligosaccharide when the Man alpha1,3 antenna was substituted with GlcNacbeta1,4.
T5 796-998 Sentence denotes Although the purified enzyme utilized the biantennary GnGn-Gp as substrate, it was twice as active with the triantennary oligosaccharide when the Man alpha1,3 antenna was substituted with GlcNacbeta1,4.
TextSentencer_T6 999-1082 Sentence denotes On the other hand the tetraantennary oligosaccharide was not a preferred substrate.
T6 999-1082 Sentence denotes On the other hand the tetraantennary oligosaccharide was not a preferred substrate.
TextSentencer_T7 1083-1209 Sentence denotes The Km values for the substrate asialo-agalactotransferrin-glycopeptide, and GDP-L-fucose were 29 and 28 microM, respectively.
T7 1083-1209 Sentence denotes The Km values for the substrate asialo-agalactotransferrin-glycopeptide, and GDP-L-fucose were 29 and 28 microM, respectively.
TextSentencer_T8 1210-1247 Sentence denotes The optimum pH of the enzyme was 6.0.
T8 1210-1247 Sentence denotes The optimum pH of the enzyme was 6.0.
TextSentencer_T9 1248-1331 Sentence denotes The activity of alpha1,6FucT was abolished in the presence of beta-mercaptoethanol.
T9 1248-1331 Sentence denotes The activity of alpha1,6FucT was abolished in the presence of beta-mercaptoethanol.
TextSentencer_T10 1332-1438 Sentence denotes Divalent cations such as Mg2+ and Ca2+ activated, but Cu2+, Zn2+ and Ni2+ strongly inhibited the activity.
T10 1332-1438 Sentence denotes Divalent cations such as Mg2+ and Ca2+ activated, but Cu2+, Zn2+ and Ni2+ strongly inhibited the activity.