| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-79 |
Sentence |
denotes |
Genomic organization of the murine polysialyltransferase gene ST8SiaIV (PST-1). |
| T1 |
0-79 |
Sentence |
denotes |
Genomic organization of the murine polysialyltransferase gene ST8SiaIV (PST-1). |
| T1 |
0-79 |
Sentence |
denotes |
Genomic organization of the murine polysialyltransferase gene ST8SiaIV (PST-1). |
| TextSentencer_T2 |
80-153 |
Sentence |
denotes |
Polysialic acid (PSA) is an important regulator of cellular interactions. |
| T2 |
80-153 |
Sentence |
denotes |
Polysialic acid (PSA) is an important regulator of cellular interactions. |
| T2 |
80-153 |
Sentence |
denotes |
Polysialic acid (PSA) is an important regulator of cellular interactions. |
| TextSentencer_T3 |
154-222 |
Sentence |
denotes |
Two enzymes (ST8SiaII and ST8SiaIV) are capable of synthesizing PSA. |
| T3 |
154-222 |
Sentence |
denotes |
Two enzymes (ST8SiaII and ST8SiaIV) are capable of synthesizing PSA. |
| T3 |
154-222 |
Sentence |
denotes |
Two enzymes (ST8SiaII and ST8SiaIV) are capable of synthesizing PSA. |
| TextSentencer_T4 |
223-327 |
Sentence |
denotes |
In the present study, the gene encoding the murine ST8SiaIV (PST-1) has been isolated and characterized. |
| T4 |
223-327 |
Sentence |
denotes |
In the present study, the gene encoding the murine ST8SiaIV (PST-1) has been isolated and characterized. |
| T4 |
223-327 |
Sentence |
denotes |
In the present study, the gene encoding the murine ST8SiaIV (PST-1) has been isolated and characterized. |
| TextSentencer_T5 |
328-488 |
Sentence |
denotes |
In contrast to the ST8SiaII (STX) gene which contains six exons and spans about 80 kb, the ST8SiaIV gene comprises only five exons spanning over at least 55 kb. |
| T5 |
328-488 |
Sentence |
denotes |
In contrast to the ST8SiaII (STX) gene which contains six exons and spans about 80 kb, the ST8SiaIV gene comprises only five exons spanning over at least 55 kb. |
| T5 |
328-488 |
Sentence |
denotes |
In contrast to the ST8SiaII (STX) gene which contains six exons and spans about 80 kb, the ST8SiaIV gene comprises only five exons spanning over at least 55 kb. |
| TextSentencer_T6 |
489-644 |
Sentence |
denotes |
However, alignment of the two genes revealed that exon-intron boundaries of exons 2-5 of ST8SiaIV and exons 3-6 of ST8SiaII are located at identical sites. |
| T6 |
489-644 |
Sentence |
denotes |
However, alignment of the two genes revealed that exon-intron boundaries of exons 2-5 of ST8SiaIV and exons 3-6 of ST8SiaII are located at identical sites. |
| T6 |
489-644 |
Sentence |
denotes |
However, alignment of the two genes revealed that exon-intron boundaries of exons 2-5 of ST8SiaIV and exons 3-6 of ST8SiaII are located at identical sites. |
| TextSentencer_T7 |
645-816 |
Sentence |
denotes |
Differences are restricted to the 5'-region encoded by one exon in the case of ST8SiaIV, whereas the corresponding region of ST8SiaII is interrupted by a very long intron. |
| T7 |
645-816 |
Sentence |
denotes |
Differences are restricted to the 5'-region encoded by one exon in the case of ST8SiaIV, whereas the corresponding region of ST8SiaII is interrupted by a very long intron. |
| T7 |
645-816 |
Sentence |
denotes |
Differences are restricted to the 5'-region encoded by one exon in the case of ST8SiaIV, whereas the corresponding region of ST8SiaII is interrupted by a very long intron. |
| TextSentencer_T8 |
817-997 |
Sentence |
denotes |
5'-RACE analysis of the ST8SiaIV transcript using mRNA from AtT20 cells identified two transcription start sites at positions -324 and -204 relative to the translation start codon. |
| T8 |
817-997 |
Sentence |
denotes |
5'-RACE analysis of the ST8SiaIV transcript using mRNA from AtT20 cells identified two transcription start sites at positions -324 and -204 relative to the translation start codon. |
| T8 |
817-997 |
Sentence |
denotes |
5'-RACE analysis of the ST8SiaIV transcript using mRNA from AtT20 cells identified two transcription start sites at positions -324 and -204 relative to the translation start codon. |
| TextSentencer_T9 |
998-1095 |
Sentence |
denotes |
The promoter region of ST8SiaIV lacks TATA- and CAAT-like sequences and is enriched in G+C (60%). |
| T9 |
998-1095 |
Sentence |
denotes |
The promoter region of ST8SiaIV lacks TATA- and CAAT-like sequences and is enriched in G+C (60%). |
| T9 |
998-1095 |
Sentence |
denotes |
The promoter region of ST8SiaIV lacks TATA- and CAAT-like sequences and is enriched in G+C (60%). |
| TextSentencer_T10 |
1096-1218 |
Sentence |
denotes |
The promoter contains putative Sp1, AP-1, AP-2, and PEA3 binding sites, as well as a purine- and a pyrimidine-rich region. |
| T10 |
1096-1218 |
Sentence |
denotes |
The promoter contains putative Sp1, AP-1, AP-2, and PEA3 binding sites, as well as a purine- and a pyrimidine-rich region. |
| T10 |
1096-1218 |
Sentence |
denotes |
The promoter contains putative Sp1, AP-1, AP-2, and PEA3 binding sites, as well as a purine- and a pyrimidine-rich region. |
| TextSentencer_T11 |
1219-1354 |
Sentence |
denotes |
Luciferase reporter gene assays demonstrated that the region between nucleotides -443 and -162 is sufficient to direct gene expression. |
| T11 |
1219-1354 |
Sentence |
denotes |
Luciferase reporter gene assays demonstrated that the region between nucleotides -443 and -162 is sufficient to direct gene expression. |
| T11 |
1219-1354 |
Sentence |
denotes |
Luciferase reporter gene assays demonstrated that the region between nucleotides -443 and -162 is sufficient to direct gene expression. |
| TextSentencer_T12 |
1355-1549 |
Sentence |
denotes |
The induction of luciferase activity was 30- and 10-fold in the PSA-positive AtT20 and CHO cells, but only 5- and 7-fold in the PSA-negative NIH-3T3 cells and in a PSA-negative subline of AtT20. |
| T12 |
1355-1549 |
Sentence |
denotes |
The induction of luciferase activity was 30- and 10-fold in the PSA-positive AtT20 and CHO cells, but only 5- and 7-fold in the PSA-negative NIH-3T3 cells and in a PSA-negative subline of AtT20. |
| T12 |
1355-1549 |
Sentence |
denotes |
The induction of luciferase activity was 30- and 10-fold in the PSA-positive AtT20 and CHO cells, but only 5- and 7-fold in the PSA-negative NIH-3T3 cells and in a PSA-negative subline of AtT20. |
| TextSentencer_T13 |
1550-1792 |
Sentence |
denotes |
Thus, although decreased in activity in PSA-negative cell lines, the basal promoter is not sufficient for the strong cell-type and tissue specific regulation of the ST8SiaIV gene, suggesting regulatory elements in the more upstream 5'-region. |
| T13 |
1550-1792 |
Sentence |
denotes |
Thus, although decreased in activity in PSA-negative cell lines, the basal promoter is not sufficient for the strong cell-type and tissue specific regulation of the ST8SiaIV gene, suggesting regulatory elements in the more upstream 5'-region. |
| T13 |
1550-1792 |
Sentence |
denotes |
Thus, although decreased in activity in PSA-negative cell lines, the basal promoter is not sufficient for the strong cell-type and tissue specific regulation of the ST8SiaIV gene, suggesting regulatory elements in the more upstream 5'-region. |