| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-94 |
Sentence |
denotes |
Expression cloning and functional characterization of human cDNA for ganglioside GM3 synthase. |
| T2 |
95-204 |
Sentence |
denotes |
Ganglioside GM3 is a major glycosphingolipid in the plasma membrane and is widely distributed in vertebrates. |
| T3 |
205-314 |
Sentence |
denotes |
We describe here the isolation of a human cDNA whose protein product is responsible for the synthesis of GM3. |
| T4 |
315-466 |
Sentence |
denotes |
The cloned cDNA spanned 2,359 base pairs, with an open reading frame encoding a protein of 362 amino acids with a predicted molecular mass of 41.7 kDa. |
| T5 |
467-671 |
Sentence |
denotes |
The deduced primary structure shows features characteristic of the sialyltransferase family, including a type II transmembrane topology and the sialylmotifs L at the center and S at the C-terminal region. |
| T6 |
672-839 |
Sentence |
denotes |
An amino acid substitution from aspartic acid to histidine was demonstrated at a position invariant in sialylmotif L of all the other sialyltransferases so far cloned. |
| T7 |
840-1059 |
Sentence |
denotes |
The best acceptor substrate for the gene product was lactosylceramide, and cells transfected with the cloned cDNA clearly exhibited de novo synthesis of GM3, with a measurable decrease in the precursor lactosylceramide. |
| T8 |
1060-1316 |
Sentence |
denotes |
Despite the ubiquitous distribution of ganglioside GM3 in human tissues, a major 2.4-kilobase transcript of the gene was found in a tissue-specific manner, with predominant expression in brain, skeletal muscle, and testis, and very low expression in liver. |
