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PubMed:9756916 JSONTXT 38 Projects

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Id Subject Object Predicate Lexical cue
T1 0-162 Sentence denotes Expression cloning and characterization of a novel murine alpha1, 3-fucosyltransferase, mFuc-TIX, that synthesizes the Lewis x (CD15) epitope in brain and kidney.
T2 163-391 Sentence denotes The 3-fucosyl-N-acetyllactosamine (Lewis x, CD15, SSEA-1) carbohydrate epitope is widely distributed in many tissues and is developmentally expressed in some rodent and human tissues, i.e. brain and lung, and mouse early embryo.
T3 392-484 Sentence denotes In such tissues, the Lewis x epitope is considered to be involved in cell-cell interactions.
T4 485-636 Sentence denotes We isolated a novel mouse alpha1,3-fucosyltransferase gene, named mFuc-TIX, from an adult mouse brain cDNA library using the expression cloning method.
T5 637-799 Sentence denotes On flow cytometric analysis, Namalwa cells transfected stably with the mFuc-TIX gene showed a marked increase in Lewis x epitopes but not sialyl Lewis x epitopes.
T6 800-996 Sentence denotes As seen experiments involving oligosaccharides as acceptor substrates, mFuc-TIX transfers a fucose to lacto-N-neotetraose but not to either alpha2,3-sialyl lacto-N-neotetraose or lacto-N-tetraose.
T7 997-1116 Sentence denotes The substrate specificity of mFuc-TIX was similar to that of mouse myeloid-type alpha1,3-fucosyltransferase (mFuc-TIV).
T8 1117-1424 Sentence denotes The deduced amino acid sequence of mFuc-TIX, consisting of 359 residues, indicated a type II membrane protein and shows low degrees of homology to the previously cloned alpha1,3-fucosyltransferases, i.e. mFuc-TIV (48.4%), mouse Fuc-TVII (39.1%), and human Fuc-TIII (43.0%), at the amino acid sequence level.
T9 1425-1608 Sentence denotes A phylogenetic tree of the alpha1, 3-fucosyltransferases constructed by the neighbor-joining method showed that mFuc-TIX is quite distant from the other alpha1, 3-fucosyltransferases.
T10 1609-1683 Sentence denotes Thus, mFuc-TIX does not belong to any subfamilies of known alpha1,3Fuc-Ts.
T11 1684-1925 Sentence denotes The mFuc-TIX transcript was mainly detected in brain and kidney with the Northern blotting and competitive reverse transcription-polymerase chain reaction methods, whereas the mFuc-TIV transcript was not detected in brain with these methods.
T12 1926-2055 Sentence denotes On in situ hybridization, the mFuc-TIX transcript was detected in neuronal cells but not in the glial cells including astrocytes.
T13 2056-2202 Sentence denotes These results strongly indicated that mFuc-TIX participates in the Lewis x synthesis in neurons of the brain and may be developmentally regulated.