| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-171 |
Sentence |
denotes |
Simple and large-scale production of N-acetylneuraminic acid from N-acetyl-D-glucosamine and pyruvate using N-acyl-D-glucosamine 2-epimerase and N-acetylneuraminate lyase. |
| T1 |
0-171 |
Sentence |
denotes |
Simple and large-scale production of N-acetylneuraminic acid from N-acetyl-D-glucosamine and pyruvate using N-acyl-D-glucosamine 2-epimerase and N-acetylneuraminate lyase. |
| TextSentencer_T2 |
172-289 |
Sentence |
denotes |
N-Acetylneuraminate lyase and N-acyl-D-glucosamine 2-epimerase had been cloned and overexpressed in Escherichia coli. |
| T2 |
172-289 |
Sentence |
denotes |
N-Acetylneuraminate lyase and N-acyl-D-glucosamine 2-epimerase had been cloned and overexpressed in Escherichia coli. |
| TextSentencer_T3 |
290-550 |
Sentence |
denotes |
Simultaneous use of these two enzymes and feeding of appropriate amounts of pyruvate to the reaction mixture made possible the high conversion of N-acetylneuraminic acid (Neu5Ac) from N-acetyl-D-glucosamine (GlcNAc) with a 77% conversion rate on a molar basis. |
| T3 |
290-550 |
Sentence |
denotes |
Simultaneous use of these two enzymes and feeding of appropriate amounts of pyruvate to the reaction mixture made possible the high conversion of N-acetylneuraminic acid (Neu5Ac) from N-acetyl-D-glucosamine (GlcNAc) with a 77% conversion rate on a molar basis. |
| TextSentencer_T4 |
551-614 |
Sentence |
denotes |
As a result, 29 kg of Neu5Ac was obtained from 27 kg of GlcNAc. |
| T4 |
551-614 |
Sentence |
denotes |
As a result, 29 kg of Neu5Ac was obtained from 27 kg of GlcNAc. |
| TextSentencer_T5 |
615-714 |
Sentence |
denotes |
The product was recovered by direct crystallization, and verified as identical to authentic Neu5Ac. |
| T5 |
615-714 |
Sentence |
denotes |
The product was recovered by direct crystallization, and verified as identical to authentic Neu5Ac. |
