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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-115 Sentence denotes Aspartic acid 252 and asparagine 185 are essential for activity of lipid N-acetylglucosaminylphosphate transferase.
T1 0-115 Sentence denotes Aspartic acid 252 and asparagine 185 are essential for activity of lipid N-acetylglucosaminylphosphate transferase.
T1 0-115 Sentence denotes Aspartic acid 252 and asparagine 185 are essential for activity of lipid N-acetylglucosaminylphosphate transferase.
TextSentencer_T2 116-403 Sentence denotes A key step in the assembly of oligosaccharide-lipid intermediates in N-linked glycosylation is the transfer of N-acetylglucosamine 1-phosphate to dolichyl phosphate, catalyzed by the enzyme UDP-N-acetylglucosaminyl:dolichyl phosphate N-acetylglucosaminyl phosphoryl transferase (L-G1PT).
T2 116-403 Sentence denotes A key step in the assembly of oligosaccharide-lipid intermediates in N-linked glycosylation is the transfer of N-acetylglucosamine 1-phosphate to dolichyl phosphate, catalyzed by the enzyme UDP-N-acetylglucosaminyl:dolichyl phosphate N-acetylglucosaminyl phosphoryl transferase (L-G1PT).
T2 116-403 Sentence denotes A key step in the assembly of oligosaccharide-lipid intermediates in N-linked glycosylation is the transfer of N-acetylglucosamine 1-phosphate to dolichyl phosphate, catalyzed by the enzyme UDP-N-acetylglucosaminyl:dolichyl phosphate N-acetylglucosaminyl phosphoryl transferase (L-G1PT).
TextSentencer_T3 404-532 Sentence denotes Comparison of the amino acid sequences of L-G1PT from five diverse species showed 75 amino acids identical in all five proteins.
T3 404-532 Sentence denotes Comparison of the amino acid sequences of L-G1PT from five diverse species showed 75 amino acids identical in all five proteins.
T3 404-532 Sentence denotes Comparison of the amino acid sequences of L-G1PT from five diverse species showed 75 amino acids identical in all five proteins.
TextSentencer_T4 533-734 Sentence denotes Using site-directed mutagenesis, we analyzed the importance of a number of these conserved residues to the enzymatic activity of L-G1PT using a plasmid shuffling procedure in Schizosaccharomyces pombe.
T4 533-734 Sentence denotes Using site-directed mutagenesis, we analyzed the importance of a number of these conserved residues to the enzymatic activity of L-G1PT using a plasmid shuffling procedure in Schizosaccharomyces pombe.
T4 533-734 Sentence denotes Using site-directed mutagenesis, we analyzed the importance of a number of these conserved residues to the enzymatic activity of L-G1PT using a plasmid shuffling procedure in Schizosaccharomyces pombe.
TextSentencer_T5 735-883 Sentence denotes S. pombe cells containing a chromosomal deletion of the essential gpt+ gene are rescued by a plasmid containing the S. pombe gpt open reading frame.
T5 735-883 Sentence denotes S. pombe cells containing a chromosomal deletion of the essential gpt+ gene are rescued by a plasmid containing the S. pombe gpt open reading frame.
T5 735-883 Sentence denotes S. pombe cells containing a chromosomal deletion of the essential gpt+ gene are rescued by a plasmid containing the S. pombe gpt open reading frame.
TextSentencer_T6 884-1066 Sentence denotes Replacement of that plasmid by a plasmid encoding a mutated hamster L-G1PT cDNA sequence indicated that the mutated protein provided sufficient enzyme activity to permit cell growth.
T6 884-1066 Sentence denotes Replacement of that plasmid by a plasmid encoding a mutated hamster L-G1PT cDNA sequence indicated that the mutated protein provided sufficient enzyme activity to permit cell growth.
T6 884-1066 Sentence denotes Replacement of that plasmid by a plasmid encoding a mutated hamster L-G1PT cDNA sequence indicated that the mutated protein provided sufficient enzyme activity to permit cell growth.
TextSentencer_T7 1067-1204 Sentence denotes Mutations of aspartic acid 252 and asparagine 185 did not allow plasmid shuffling, indicating these residues were essential for activity.
T7 1067-1204 Sentence denotes Mutations of aspartic acid 252 and asparagine 185 did not allow plasmid shuffling, indicating these residues were essential for activity.
T7 1067-1204 Sentence denotes Mutations of aspartic acid 252 and asparagine 185 did not allow plasmid shuffling, indicating these residues were essential for activity.
TextSentencer_T8 1205-1336 Sentence denotes A combination of mutations at asparagine 182 and tryptophan 122 did not allow plasmid shuffling, although the single mutations did.
T8 1205-1336 Sentence denotes A combination of mutations at asparagine 182 and tryptophan 122 did not allow plasmid shuffling, although the single mutations did.
T8 1205-1336 Sentence denotes A combination of mutations at asparagine 182 and tryptophan 122 did not allow plasmid shuffling, although the single mutations did.
TextSentencer_T9 1337-1526 Sentence denotes Overexpression of the mutant proteins in S. pombe conferred tunicamycin (TM) resistance, indicating that the mutant proteins had a conformation necessary for binding TM, a substrate analog.
T9 1337-1526 Sentence denotes Overexpression of the mutant proteins in S. pombe conferred tunicamycin (TM) resistance, indicating that the mutant proteins had a conformation necessary for binding TM, a substrate analog.
T9 1337-1526 Sentence denotes Overexpression of the mutant proteins in S. pombe conferred tunicamycin (TM) resistance, indicating that the mutant proteins had a conformation necessary for binding TM, a substrate analog.
TextSentencer_T10 1527-1638 Sentence denotes The mutant proteins were also detected in Western blots and were correctly localized to the membrane fractions.
T10 1527-1638 Sentence denotes The mutant proteins were also detected in Western blots and were correctly localized to the membrane fractions.
T10 1527-1638 Sentence denotes The mutant proteins were also detected in Western blots and were correctly localized to the membrane fractions.
TextSentencer_T11 1639-1795 Sentence denotes However, the overexpressed proteins did not increase the endogenous level of enzymatic activity in these cells, indicating they were enzymatically inactive.
T11 1639-1795 Sentence denotes However, the overexpressed proteins did not increase the endogenous level of enzymatic activity in these cells, indicating they were enzymatically inactive.
T11 1639-1795 Sentence denotes However, the overexpressed proteins did not increase the endogenous level of enzymatic activity in these cells, indicating they were enzymatically inactive.