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PubMed:9451016 JSONTXT 54 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-188 Sentence denotes Cloning and functional expression of the human GlcNAc-1-P transferase, the enzyme for the committed step of the dolichol cycle, by heterologous complementation in Saccharomyces cerevisiae.
T1 0-188 Sentence denotes Cloning and functional expression of the human GlcNAc-1-P transferase, the enzyme for the committed step of the dolichol cycle, by heterologous complementation in Saccharomyces cerevisiae.
T1 0-188 Sentence denotes Cloning and functional expression of the human GlcNAc-1-P transferase, the enzyme for the committed step of the dolichol cycle, by heterologous complementation in Saccharomyces cerevisiae.
TextSentencer_T2 189-322 Sentence denotes The gene for the human dolichol cycle GlcNAc-1-P transferase (ALG7/GPT) was cloned by screening a human lung fibroblast cDNA library.
T2 189-322 Sentence denotes The gene for the human dolichol cycle GlcNAc-1-P transferase (ALG7/GPT) was cloned by screening a human lung fibroblast cDNA library.
T2 189-322 Sentence denotes The gene for the human dolichol cycle GlcNAc-1-P transferase (ALG7/GPT) was cloned by screening a human lung fibroblast cDNA library.
TextSentencer_T3 323-514 Sentence denotes The library was constructed in a Saccharomyces cerevisiae expression vector, and the positive clone was identified by complementation of the conditional lethal S.cerevisiae strain YPH-A7-GAL.
T3 323-514 Sentence denotes The library was constructed in a Saccharomyces cerevisiae expression vector, and the positive clone was identified by complementation of the conditional lethal S.cerevisiae strain YPH-A7-GAL.
T3 323-514 Sentence denotes The library was constructed in a Saccharomyces cerevisiae expression vector, and the positive clone was identified by complementation of the conditional lethal S.cerevisiae strain YPH-A7-GAL.
TextSentencer_T4 515-639 Sentence denotes This strain was constructed by replacing the endogenous promoter of the GPT-gene by the stringently regulated GAL1-promoter.
T4 515-639 Sentence denotes This strain was constructed by replacing the endogenous promoter of the GPT-gene by the stringently regulated GAL1-promoter.
T4 515-639 Sentence denotes This strain was constructed by replacing the endogenous promoter of the GPT-gene by the stringently regulated GAL1-promoter.
TextSentencer_T5 640-718 Sentence denotes This construct allows to specifically suppress the endogenous enzyme activity.
T5 640-718 Sentence denotes This construct allows to specifically suppress the endogenous enzyme activity.
T5 640-718 Sentence denotes This construct allows to specifically suppress the endogenous enzyme activity.
TextSentencer_T6 719-901 Sentence denotes The insert of the positive clone displayed an open reading frame of 1200 nucleotides, coding for a putative protein of 400 amino acids with a calculated molecular weight of 44.7 kDa.
T6 719-901 Sentence denotes The insert of the positive clone displayed an open reading frame of 1200 nucleotides, coding for a putative protein of 400 amino acids with a calculated molecular weight of 44.7 kDa.
T6 719-901 Sentence denotes The insert of the positive clone displayed an open reading frame of 1200 nucleotides, coding for a putative protein of 400 amino acids with a calculated molecular weight of 44.7 kDa.
TextSentencer_T7 902-1088 Sentence denotes The deduced protein sequence shows a homology of over 90% when compared with other mammalian GPT sequences, thus resembling the close phylogenetic relationship between mammalian species.
T7 902-1088 Sentence denotes The deduced protein sequence shows a homology of over 90% when compared with other mammalian GPT sequences, thus resembling the close phylogenetic relationship between mammalian species.
T7 902-1088 Sentence denotes The deduced protein sequence shows a homology of over 90% when compared with other mammalian GPT sequences, thus resembling the close phylogenetic relationship between mammalian species.
TextSentencer_T8 1089-1259 Sentence denotes This homology however decreases to 40-50% when compared to more distantly related organisms such as S.cerevisiae , Schizosaccharomyces pombe , or Leishmania amazonensis .
T8 1089-1259 Sentence denotes This homology however decreases to 40-50% when compared to more distantly related organisms such as S.cerevisiae , Schizosaccharomyces pombe , or Leishmania amazonensis .
T8 1089-1259 Sentence denotes This homology however decreases to 40-50% when compared to more distantly related organisms such as S.cerevisiae , Schizosaccharomyces pombe , or Leishmania amazonensis .
TextSentencer_T9 1260-1395 Sentence denotes Biochemical characterization of the recombinant protein showed that it is functionally expressed in the S.cerevisiae strain YPH-A7-GAL.
T9 1260-1395 Sentence denotes Biochemical characterization of the recombinant protein showed that it is functionally expressed in the S.cerevisiae strain YPH-A7-GAL.
T9 1260-1395 Sentence denotes Biochemical characterization of the recombinant protein showed that it is functionally expressed in the S.cerevisiae strain YPH-A7-GAL.
TextSentencer_T10 1396-1613 Sentence denotes GlcNAc- and GlcNAc2-PP-Dolichol biosynthesis could be shown with isolated S.cerevisiae membranes from cells harboring the recombinant plasmid and grown on glucose thus suppressing transcription of the endogenous gene.
T10 1396-1613 Sentence denotes GlcNAc- and GlcNAc2-PP-Dolichol biosynthesis could be shown with isolated S.cerevisiae membranes from cells harboring the recombinant plasmid and grown on glucose thus suppressing transcription of the endogenous gene.
T10 1396-1613 Sentence denotes GlcNAc- and GlcNAc2-PP-Dolichol biosynthesis could be shown with isolated S.cerevisiae membranes from cells harboring the recombinant plasmid and grown on glucose thus suppressing transcription of the endogenous gene.
TextSentencer_T11 1614-1698 Sentence denotes Synthesis could be stimulated by dolicholphosphate and was inhibited by tunicamycin.
T11 1614-1698 Sentence denotes Synthesis could be stimulated by dolicholphosphate and was inhibited by tunicamycin.
T11 1614-1698 Sentence denotes Synthesis could be stimulated by dolicholphosphate and was inhibited by tunicamycin.
TextSentencer_T12 1699-1940 Sentence denotes These results show that we have cloned the human GlcNAc-1-P transferase by heterologous complementation in S. cerevisiae, a strategy that may be useful for the cloning and characterization of glycosyltransferases from a variety of organisms.
T12 1699-1940 Sentence denotes These results show that we have cloned the human GlcNAc-1-P transferase by heterologous complementation in S. cerevisiae, a strategy that may be useful for the cloning and characterization of glycosyltransferases from a variety of organisms.
T12 1699-1940 Sentence denotes These results show that we have cloned the human GlcNAc-1-P transferase by heterologous complementation in S. cerevisiae, a strategy that may be useful for the cloning and characterization of glycosyltransferases from a variety of organisms.