| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-87 |
Sentence |
denotes |
Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase. |
| T2 |
88-188 |
Sentence |
denotes |
We have previously cloned chondroitin 6-sulfotransferase (C6ST) cDNA from chick embryo chondrocytes. |
| T3 |
189-295 |
Sentence |
denotes |
C6ST catalyzes sulfation of chondroitin, keratan sulfate, and sialyl N-acetyllactosamine oligosaccharides. |
| T4 |
296-426 |
Sentence |
denotes |
In this study, we report the cloning and characterization of a novel sulfotransferase that catalyzes sulfation of keratan sulfate. |
| T5 |
427-554 |
Sentence |
denotes |
This new sulfotransferase cDNA clone was obtained from a human fetal brain library by cross-hybridization with chick C6ST cDNA. |
| T6 |
555-698 |
Sentence |
denotes |
The cDNA clone obtained contains a single open reading frame that predicts a type II transmembrane protein composed of 411 amino acid residues. |
| T7 |
699-918 |
Sentence |
denotes |
When the cDNA was introduced into a eukaryotic expression vector and transfected in COS-7 cells, keratan sulfate sulfotransferase activity was overexpressed, but C6ST activity was not increased over that of the control. |
| T8 |
919-1191 |
Sentence |
denotes |
Structural analysis of 35S-labeled glycosaminoglycan, which was formed from keratan sulfate by the reaction with 35S-labeled 3'-phosphoadenosine 5'-phosphosulfate and the recombinant sulfotransferase, showed that keratan sulfate was sulfated at position 6 of Gal residues. |
| T9 |
1192-1355 |
Sentence |
denotes |
On the basis of the acceptor substrate specificity, we propose keratan sulfate Gal-6-sulfotransferase (KSGal6ST) for the name of the newly cloned sulfotransferase. |
| T10 |
1356-1398 |
Sentence |
denotes |
KSGal6ST was assigned to chromosome 11p11. |
| T11 |
1399-1444 |
Sentence |
denotes |
1-11.2 by fluorescence in situ hybridization. |
| T12 |
1445-1549 |
Sentence |
denotes |
Among various human adult tissues, a 2.8-kilobase message of KSGal6ST was expressed mainly in the brain. |
| T13 |
1550-1727 |
Sentence |
denotes |
When poly(A)+ RNAs from the chick embryo cornea and brain were probed with the human KSGal6ST cDNA in Northern hybridization, a clear band with about 2.8 kilobases was detected. |
| T14 |
1728-1848 |
Sentence |
denotes |
These observations suggest that KSGal6ST may participate in the biosynthesis of keratan sulfate in the brain and cornea. |
