| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-47 |
Sentence |
denotes |
A family of human beta4-galactosyltransferases. |
| T2 |
48-184 |
Sentence |
denotes |
Cloning and expression of two novel UDP-galactose:beta-n-acetylglucosamine beta1, 4-galactosyltransferases, beta4Gal-T2 and beta4Gal-T3. |
| T3 |
185-444 |
Sentence |
denotes |
BLAST analysis of expressed sequence tags (ESTs) using the coding sequence of the human UDP-galactose:beta-N-acetylglucosamine beta1, 4-galactosyltransferase, designated beta4Gal-T1, revealed a large number of ESTs with identical as well as similar sequences. |
| T4 |
445-559 |
Sentence |
denotes |
ESTs with sequences similar to that of beta4Gal-T1 could be grouped into at least two non-identical sequence sets. |
| T5 |
560-782 |
Sentence |
denotes |
Analysis of the predicted amino acid sequence of the novel ESTs with beta4Gal-T1 revealed conservation of short sequence motifs as well as cysteine residues previously shown to be important for the function of beta4Gal-T1. |
| T6 |
783-978 |
Sentence |
denotes |
The likelihood that the identified ESTs represented novel galactosyltransferase genes was tested by cloning and sequencing of the full coding region of two distinct genes, followed by expression. |
| T7 |
979-1207 |
Sentence |
denotes |
Expression of soluble secreted constructs in the baculovirus system showed that these genes represented genuine UDP-galactose:beta-N-acetylglucosamine beta1, 4-galactosyltransferases, thus designated beta4Gal-T2 and beta4Gal-T3. |
| T8 |
1208-1319 |
Sentence |
denotes |
Genomic cloning of the genes revealed that they have identical genomic organizations compared with beta4Gal-T1. |
| T9 |
1320-1373 |
Sentence |
denotes |
The two novel genes were located on 1p32-33 and 1q23. |
| T10 |
1374-1484 |
Sentence |
denotes |
The results demonstrate the existence of a family of homologous galactosyltransferases with related functions. |
| T11 |
1485-1673 |
Sentence |
denotes |
The existence of multiple beta4-galactosyltransferases with the same or overlapping functions may be relevant for interpretation of biological functions previously assigned to beta4Gal-T1. |
