| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-43 |
Sentence |
denotes |
The yeast CWH41 gene encodes glucosidase I. |
| T1 |
0-43 |
Sentence |
denotes |
The yeast CWH41 gene encodes glucosidase I. |
| T1 |
0-43 |
Sentence |
denotes |
The yeast CWH41 gene encodes glucosidase I. |
| TextSentencer_T2 |
44-282 |
Sentence |
denotes |
N-Glycosylation in the yeast Saccharomyces cerevisiae entails the synthesis of a Glc3Man9GlcNAc2 oligosaccharide precursor which is subsequently transferred to suitable protein acceptors, a process which is conserved among all eukaryotes. |
| T2 |
44-282 |
Sentence |
denotes |
N-Glycosylation in the yeast Saccharomyces cerevisiae entails the synthesis of a Glc3Man9GlcNAc2 oligosaccharide precursor which is subsequently transferred to suitable protein acceptors, a process which is conserved among all eukaryotes. |
| T2 |
44-282 |
Sentence |
denotes |
N-Glycosylation in the yeast Saccharomyces cerevisiae entails the synthesis of a Glc3Man9GlcNAc2 oligosaccharide precursor which is subsequently transferred to suitable protein acceptors, a process which is conserved among all eukaryotes. |
| TextSentencer_T3 |
283-481 |
Sentence |
denotes |
Processing of the oligosaccharide occurs immediately following this transfer, the first step being the removal of the terminal alpha-1,2-linked glucose by glucosidase I in the endoplasmic reticulum. |
| T3 |
283-481 |
Sentence |
denotes |
Processing of the oligosaccharide occurs immediately following this transfer, the first step being the removal of the terminal alpha-1,2-linked glucose by glucosidase I in the endoplasmic reticulum. |
| T3 |
283-481 |
Sentence |
denotes |
Processing of the oligosaccharide occurs immediately following this transfer, the first step being the removal of the terminal alpha-1,2-linked glucose by glucosidase I in the endoplasmic reticulum. |
| TextSentencer_T4 |
482-594 |
Sentence |
denotes |
Although yeast glucosidase I has been isolated, the yeast gene encoding this enzyme has not yet been identified. |
| T4 |
482-594 |
Sentence |
denotes |
Although yeast glucosidase I has been isolated, the yeast gene encoding this enzyme has not yet been identified. |
| T4 |
482-594 |
Sentence |
denotes |
Although yeast glucosidase I has been isolated, the yeast gene encoding this enzyme has not yet been identified. |
| TextSentencer_T5 |
595-826 |
Sentence |
denotes |
In the present work, it is shown that Cwh41p, a yeast endoplasmic reticulum protein previously identified as being required for normal cell wall beta-1,6-glucan synthesis (Jiang, Sheraton, Ram, Dijkgraaf, Klis, and Bussey (1996) J. |
| T5 |
595-826 |
Sentence |
denotes |
In the present work, it is shown that Cwh41p, a yeast endoplasmic reticulum protein previously identified as being required for normal cell wall beta-1,6-glucan synthesis (Jiang, Sheraton, Ram, Dijkgraaf, Klis, and Bussey (1996) J. |
| T5 |
595-941 |
Sentence |
denotes |
In the present work, it is shown that Cwh41p, a yeast endoplasmic reticulum protein previously identified as being required for normal cell wall beta-1,6-glucan synthesis (Jiang, Sheraton, Ram, Dijkgraaf, Klis, and Bussey (1996) J. Bacteriol., 178, 1162-1171), has significant amino acid similarity to the product of the human glucosidase I cDNA. |
| TextSentencer_T6 |
827-941 |
Sentence |
denotes |
Bacteriol., 178, 1162-1171), has significant amino acid similarity to the product of the human glucosidase I cDNA. |
| T6 |
827-941 |
Sentence |
denotes |
Bacteriol., 178, 1162-1171), has significant amino acid similarity to the product of the human glucosidase I cDNA. |
| TextSentencer_T7 |
942-1104 |
Sentence |
denotes |
Tetrad analysis for glucosidase I activity in vitro and in vivo was done on the progeny from the spores obtained from the heterozygous diploid, cwh41 delta::HIS3. |
| T6 |
942-1104 |
Sentence |
denotes |
Tetrad analysis for glucosidase I activity in vitro and in vivo was done on the progeny from the spores obtained from the heterozygous diploid, cwh41 delta::HIS3. |
| T7 |
942-1104 |
Sentence |
denotes |
Tetrad analysis for glucosidase I activity in vitro and in vivo was done on the progeny from the spores obtained from the heterozygous diploid, cwh41 delta::HIS3. |
| TextSentencer_T8 |
1105-1350 |
Sentence |
denotes |
It is shown that, unlike CWH41 cells, cell extracts obtained from cwh41 delta null mutants are unable to release glucose residues from the synthetic trisaccharide substrate alpha-D-Glc 1-->2 alpha-D-Glc 1-->3 alpha-D-Glc-O(CH2)8 COOCH3 in vitro. |
| T7 |
1105-1350 |
Sentence |
denotes |
It is shown that, unlike CWH41 cells, cell extracts obtained from cwh41 delta null mutants are unable to release glucose residues from the synthetic trisaccharide substrate alpha-D-Glc 1-->2 alpha-D-Glc 1-->3 alpha-D-Glc-O(CH2)8 COOCH3 in vitro. |
| T8 |
1105-1350 |
Sentence |
denotes |
It is shown that, unlike CWH41 cells, cell extracts obtained from cwh41 delta null mutants are unable to release glucose residues from the synthetic trisaccharide substrate alpha-D-Glc 1-->2 alpha-D-Glc 1-->3 alpha-D-Glc-O(CH2)8 COOCH3 in vitro. |
| TextSentencer_T9 |
1351-1795 |
Sentence |
denotes |
Following 1 h labeling of cells with [3H]mannose, analysis by high pressure liquid chromatography of the labeled N-linked oligosaccharides, combined with treatment with jack bean alpha mannosidase and yeast glucosidase I, shows that the oligosaccharides isolated form a cwh41 delta null mutant are fully glucosylated, retaining the three terminal glucose residues, whereas the oligosaccharides from CWH41 cells do not have any glucose residues. |
| T8 |
1351-1795 |
Sentence |
denotes |
Following 1 h labeling of cells with [3H]mannose, analysis by high pressure liquid chromatography of the labeled N-linked oligosaccharides, combined with treatment with jack bean alpha mannosidase and yeast glucosidase I, shows that the oligosaccharides isolated form a cwh41 delta null mutant are fully glucosylated, retaining the three terminal glucose residues, whereas the oligosaccharides from CWH41 cells do not have any glucose residues. |
| T9 |
1351-1795 |
Sentence |
denotes |
Following 1 h labeling of cells with [3H]mannose, analysis by high pressure liquid chromatography of the labeled N-linked oligosaccharides, combined with treatment with jack bean alpha mannosidase and yeast glucosidase I, shows that the oligosaccharides isolated form a cwh41 delta null mutant are fully glucosylated, retaining the three terminal glucose residues, whereas the oligosaccharides from CWH41 cells do not have any glucose residues. |
| TextSentencer_T10 |
1796-1992 |
Sentence |
denotes |
These results showing a lack of glucosidase I activity in cwh41 delta null mutants both in vitro and in vivo are consistent with the structural evidence that CWH41 encodes the yeast glucosidase I. |
| T9 |
1796-1992 |
Sentence |
denotes |
These results showing a lack of glucosidase I activity in cwh41 delta null mutants both in vitro and in vivo are consistent with the structural evidence that CWH41 encodes the yeast glucosidase I. |
| T10 |
1796-1992 |
Sentence |
denotes |
These results showing a lack of glucosidase I activity in cwh41 delta null mutants both in vitro and in vivo are consistent with the structural evidence that CWH41 encodes the yeast glucosidase I. |