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PubMed:9153228 JSONTXT 41 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-161 Sentence denotes Isolation and characterization of a new member of the scavenger receptor superfamily, glycoprotein-340 (gp-340), as a lung surfactant protein-D binding molecule.
T1 0-161 Sentence denotes Isolation and characterization of a new member of the scavenger receptor superfamily, glycoprotein-340 (gp-340), as a lung surfactant protein-D binding molecule.
T2 162-633 Sentence denotes We have purified a previously unknown glycoprotein (designated gp-340) from human bronchioalveolar lung washings from a patient with alveolar proteinosis. gp-340 was identified by its calcium-dependent binding to lung surfactant protein D (SP-D) and by its molecular mass of 340 kDa in the reduced state on SDS-polyacrylamide gel electrophoresis (PAGE). gp-340 was purified from the 10,000 x g pellet of the lavage fluid by ion-exchange and gel permeation chromatography.
T2 162-633 Sentence denotes We have purified a previously unknown glycoprotein (designated gp-340) from human bronchioalveolar lung washings from a patient with alveolar proteinosis. gp-340 was identified by its calcium-dependent binding to lung surfactant protein D (SP-D) and by its molecular mass of 340 kDa in the reduced state on SDS-polyacrylamide gel electrophoresis (PAGE). gp-340 was purified from the 10,000 x g pellet of the lavage fluid by ion-exchange and gel permeation chromatography.
T3 634-722 Sentence denotes On SDS-PAGE, gp-340 showed an apparent molecular mass of 290 kDa in the unreduced state.
T3 634-722 Sentence denotes On SDS-PAGE, gp-340 showed an apparent molecular mass of 290 kDa in the unreduced state.
T4 723-832 Sentence denotes On gel chromatography under non-dissociating conditions, the apparent molecular mass of gp-340 was >1000 kDa.
T4 723-832 Sentence denotes On gel chromatography under non-dissociating conditions, the apparent molecular mass of gp-340 was >1000 kDa.
T5 833-1011 Sentence denotes The presence of N-linked glycosylation was shown by digestion with N-glycosidase F, which reduced the apparent molecular mass of gp-340 under reducing condition to about 300 kDa.
T5 833-1011 Sentence denotes The presence of N-linked glycosylation was shown by digestion with N-glycosidase F, which reduced the apparent molecular mass of gp-340 under reducing condition to about 300 kDa.
T6 1012-1100 Sentence denotes Partial amino acid sequence data showed the presence of scavenger-receptor type domains.
T6 1012-1100 Sentence denotes Partial amino acid sequence data showed the presence of scavenger-receptor type domains.
T7 1101-1225 Sentence denotes Monoclonal and polyclonal antibodies were raised against gp-340, and their specificities were confirmed by Western blotting.
T7 1101-1225 Sentence denotes Monoclonal and polyclonal antibodies were raised against gp-340, and their specificities were confirmed by Western blotting.
T8 1226-1380 Sentence denotes The antibodies were used for immunohistochemical localization of gp-340 in the lung, where it was found on the surface of and within alveolar macrophages.
T8 1226-1380 Sentence denotes The antibodies were used for immunohistochemical localization of gp-340 in the lung, where it was found on the surface of and within alveolar macrophages.
T9 1381-1531 Sentence denotes Direct binding between gp-340 and SP-D took place at physiological ionic strength, required the presence of calcium, and was not inhibited by maltose.
T9 1381-1531 Sentence denotes Direct binding between gp-340 and SP-D took place at physiological ionic strength, required the presence of calcium, and was not inhibited by maltose.
T10 1532-1664 Sentence denotes The binding between SP-D and mannan also required the presence of calcium, but this interaction was completely inhibited by maltose.
T10 1532-1664 Sentence denotes The binding between SP-D and mannan also required the presence of calcium, but this interaction was completely inhibited by maltose.
T11 1665-1821 Sentence denotes The same binding pattern was seen between gp-340 and recombinant human SP-D composed of the trimeric neck region and three carbohydrate recognition domains.
T11 1665-1821 Sentence denotes The same binding pattern was seen between gp-340 and recombinant human SP-D composed of the trimeric neck region and three carbohydrate recognition domains.
T12 1822-2057 Sentence denotes These findings indicate that the binding between gp-340 and SP-D is a protein-protein interaction rather than a lectin-carbohydrate interaction and that the binding to gp-340 takes place via the carbohydrate recognition domain of SP-D.
T12 1822-2057 Sentence denotes These findings indicate that the binding between gp-340 and SP-D is a protein-protein interaction rather than a lectin-carbohydrate interaction and that the binding to gp-340 takes place via the carbohydrate recognition domain of SP-D.
T13 2058-2193 Sentence denotes We conclude that gp-340 is a new member of the scavenger-receptor superfamily and likely to be a truncated form of a receptor for SP-D.
T13 2058-2193 Sentence denotes We conclude that gp-340 is a new member of the scavenger-receptor superfamily and likely to be a truncated form of a receptor for SP-D.