| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-94 |
Sentence |
denotes |
Enzymatic sulfation of galactose residue of keratan sulfate by chondroitin 6-sulfotransferase. |
| T1 |
0-94 |
Sentence |
denotes |
Enzymatic sulfation of galactose residue of keratan sulfate by chondroitin 6-sulfotransferase. |
| T1 |
0-94 |
Sentence |
denotes |
Enzymatic sulfation of galactose residue of keratan sulfate by chondroitin 6-sulfotransferase. |
| TextSentencer_T2 |
95-500 |
Sentence |
denotes |
We have previously found that the purified chondroitin 6-sulfotransferase (C6ST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of N-acetylgalactosamine in chondroitin, catalyzed the sulfation of keratan sulfate, and that both the C6ST activity and the keratan sulfate sulfotransferase (KSST) activity were expressed in COS-7 cells when C6ST cDNA was transfected. |
| T2 |
95-500 |
Sentence |
denotes |
We have previously found that the purified chondroitin 6-sulfotransferase (C6ST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of N-acetylgalactosamine in chondroitin, catalyzed the sulfation of keratan sulfate, and that both the C6ST activity and the keratan sulfate sulfotransferase (KSST) activity were expressed in COS-7 cells when C6ST cDNA was transfected. |
| T2 |
95-500 |
Sentence |
denotes |
We have previously found that the purified chondroitin 6-sulfotransferase (C6ST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of N-acetylgalactosamine in chondroitin, catalyzed the sulfation of keratan sulfate, and that both the C6ST activity and the keratan sulfate sulfotransferase (KSST) activity were expressed in COS-7 cells when C6ST cDNA was transfected. |
| TextSentencer_T3 |
501-705 |
Sentence |
denotes |
In this report we describe some properties of the KSST activity contained in the purified C6ST, and characterize the sulfated products formed from keratan sulfate and partially desulfated keratan sulfate. |
| T3 |
501-705 |
Sentence |
denotes |
In this report we describe some properties of the KSST activity contained in the purified C6ST, and characterize the sulfated products formed from keratan sulfate and partially desulfated keratan sulfate. |
| T3 |
501-705 |
Sentence |
denotes |
In this report we describe some properties of the KSST activity contained in the purified C6ST, and characterize the sulfated products formed from keratan sulfate and partially desulfated keratan sulfate. |
| TextSentencer_T4 |
706-846 |
Sentence |
denotes |
Optimal pH, requirement for cationic activators, and Km value for PAPS of the KSST activity were very similar to those of the C6ST activity. |
| T4 |
706-846 |
Sentence |
denotes |
Optimal pH, requirement for cationic activators, and Km value for PAPS of the KSST activity were very similar to those of the C6ST activity. |
| T4 |
706-846 |
Sentence |
denotes |
Optimal pH, requirement for cationic activators, and Km value for PAPS of the KSST activity were very similar to those of the C6ST activity. |
| TextSentencer_T5 |
847-1101 |
Sentence |
denotes |
35S-Labeled glycosaminoglycans formed from keratan sulfate and partially desulfated keratan sulfate were N-deacetylated by treatment with hydrazine/hydrazine sulfate and then cleaved with HNO2 at pH 4, and the resulting products were reduced with NaB3H4. |
| T5 |
847-1101 |
Sentence |
denotes |
35S-Labeled glycosaminoglycans formed from keratan sulfate and partially desulfated keratan sulfate were N-deacetylated by treatment with hydrazine/hydrazine sulfate and then cleaved with HNO2 at pH 4, and the resulting products were reduced with NaB3H4. |
| T5 |
847-1101 |
Sentence |
denotes |
35S-Labeled glycosaminoglycans formed from keratan sulfate and partially desulfated keratan sulfate were N-deacetylated by treatment with hydrazine/hydrazine sulfate and then cleaved with HNO2 at pH 4, and the resulting products were reduced with NaB3H4. |
| TextSentencer_T6 |
1102-1398 |
Sentence |
denotes |
Analysis of the degradation products with paper chromatography and high performance liquid chromatography provided evidence that C6ST transferred sulfate to position 6 of galactose residue which was glycosidically linked to N-acetylglucosamine 6-sulfate residue or to N-acetylglucosamine residue. |
| T6 |
1102-1398 |
Sentence |
denotes |
Analysis of the degradation products with paper chromatography and high performance liquid chromatography provided evidence that C6ST transferred sulfate to position 6 of galactose residue which was glycosidically linked to N-acetylglucosamine 6-sulfate residue or to N-acetylglucosamine residue. |
| T6 |
1102-1398 |
Sentence |
denotes |
Analysis of the degradation products with paper chromatography and high performance liquid chromatography provided evidence that C6ST transferred sulfate to position 6 of galactose residue which was glycosidically linked to N-acetylglucosamine 6-sulfate residue or to N-acetylglucosamine residue. |
| TextSentencer_T7 |
1399-1617 |
Sentence |
denotes |
Northern blot analysis using poly (A)+ RNA from 12-d-old chick embryos indicated that the message of C6ST was expressed not only in the cartilage but also in the cornea in which keratan sulfate is actively synthesized. |
| T7 |
1399-1617 |
Sentence |
denotes |
Northern blot analysis using poly (A)+ RNA from 12-d-old chick embryos indicated that the message of C6ST was expressed not only in the cartilage but also in the cornea in which keratan sulfate is actively synthesized. |
| T7 |
1399-1617 |
Sentence |
denotes |
Northern blot analysis using poly (A)+ RNA from 12-d-old chick embryos indicated that the message of C6ST was expressed not only in the cartilage but also in the cornea in which keratan sulfate is actively synthesized. |