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PubMed:8910600 JSONTXT 39 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V).
T1 0-90 Sentence denotes Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V).
T2 91-192 Sentence denotes Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3.
T2 91-192 Sentence denotes Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3.
T3 193-557 Sentence denotes The cDNAs encoding a new alpha2,8-sialyltransferase (ST8Sia V) were cloned from a mouse brain cDNA library by means of a polymerase chain reaction-based method using the nucleotide sequence information on mouse ST8Sia I (GD3 synthase) and mouse ST8Sia III (Siaalpha2,3Galbeta1,4GlcNAcalpha2,8-sialyltransferase ), both of which exhibit activity toward glycolipids.
T3 193-557 Sentence denotes The cDNAs encoding a new alpha2,8-sialyltransferase (ST8Sia V) were cloned from a mouse brain cDNA library by means of a polymerase chain reaction-based method using the nucleotide sequence information on mouse ST8Sia I (GD3 synthase) and mouse ST8Sia III (Siaalpha2,3Galbeta1,4GlcNAcalpha2,8-sialyltransferase ), both of which exhibit activity toward glycolipids.
T4 558-684 Sentence denotes The predicted amino acid sequence of ST8Sia V shows 36.1% and 15.0% identity to those of mouse ST8Sia I and III, respectively.
T4 558-684 Sentence denotes The predicted amino acid sequence of ST8Sia V shows 36.1% and 15.0% identity to those of mouse ST8Sia I and III, respectively.
T5 685-891 Sentence denotes The recombinant protein A-fused ST8Sia V expressed in COS-7 cells exhibited an alpha2, 8-sialyltransferase activity toward GM1b, GD1a, GT1b, and GD3, and synthesized GD1c, GT1a, GQ1b, and GT3, respectively.
T5 685-891 Sentence denotes The recombinant protein A-fused ST8Sia V expressed in COS-7 cells exhibited an alpha2, 8-sialyltransferase activity toward GM1b, GD1a, GT1b, and GD3, and synthesized GD1c, GT1a, GQ1b, and GT3, respectively.
T6 892-994 Sentence denotes The apparent Km values for GM1b, GD1a, GT1b and GD3 were 1.1, 0.082, 0.070, and 0.28 mM, respectively.
T6 892-994 Sentence denotes The apparent Km values for GM1b, GD1a, GT1b and GD3 were 1.1, 0.082, 0.070, and 0.28 mM, respectively.
T7 995-1049 Sentence denotes However, ST8Sia V did not exhibit activity toward GM3.
T7 995-1049 Sentence denotes However, ST8Sia V did not exhibit activity toward GM3.
T8 1050-1181 Sentence denotes Thus, the substrate specificity of ST8Sia V is different from those of ST8Sia I and III, both of which exhibit activity toward GM3.
T8 1050-1181 Sentence denotes Thus, the substrate specificity of ST8Sia V is different from those of ST8Sia I and III, both of which exhibit activity toward GM3.
T9 1182-1464 Sentence denotes Transfection of the ST8Sia V gene into COS-7 cells, which express GD1a as a major glycolipid, led to the expression of determinants for monoclonal antibody 4F10, which recognizes GT1a and GQ1b, suggesting that ST8Sia V exhibits activity toward gangliosides GD1a and/or GT1b in vivo.
T9 1182-1464 Sentence denotes Transfection of the ST8Sia V gene into COS-7 cells, which express GD1a as a major glycolipid, led to the expression of determinants for monoclonal antibody 4F10, which recognizes GT1a and GQ1b, suggesting that ST8Sia V exhibits activity toward gangliosides GD1a and/or GT1b in vivo.
T10 1465-1626 Sentence denotes The expression of the ST8Sia V gene was tissue- and developmental stage-specific, and was clearly different from those of other alpha2,8-sialyltransferase genes.
T10 1465-1626 Sentence denotes The expression of the ST8Sia V gene was tissue- and developmental stage-specific, and was clearly different from those of other alpha2,8-sialyltransferase genes.
T11 1627-1727 Sentence denotes The ST8Sia V gene was strongly expressed in the brain and weakly in other tissues such as the liver.
T11 1627-1727 Sentence denotes The ST8Sia V gene was strongly expressed in the brain and weakly in other tissues such as the liver.
T12 1728-1798 Sentence denotes In addition, its expression was greater in the adult than fetal brain.
T12 1728-1798 Sentence denotes In addition, its expression was greater in the adult than fetal brain.
T13 1799-1950 Sentence denotes These results strongly indicate that ST8Sia V is a candidate for SAT-V, the alpha2,8-sialyltransferase involved in GD1c, GT1a, GQ1b, and GT3 synthesis.
T13 1799-1950 Sentence denotes These results strongly indicate that ST8Sia V is a candidate for SAT-V, the alpha2,8-sialyltransferase involved in GD1c, GT1a, GQ1b, and GT3 synthesis.