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PubMed:8842710 JSONTXT 53 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-90 Sentence denotes Trehalose-P synthase of mycobacteria: its substrate specificity is affected by polyanions.
T1 0-90 Sentence denotes Trehalose-P synthase of mycobacteria: its substrate specificity is affected by polyanions.
T1 0-90 Sentence denotes Trehalose-P synthase of mycobacteria: its substrate specificity is affected by polyanions.
TextSentencer_T2 91-206 Sentence denotes The trehalose-P synthase was purified to near homogeneity from the cytoplasmic fraction of Mycobacterium smegmatis.
T2 91-206 Sentence denotes The trehalose-P synthase was purified to near homogeneity from the cytoplasmic fraction of Mycobacterium smegmatis.
T2 91-206 Sentence denotes The trehalose-P synthase was purified to near homogeneity from the cytoplasmic fraction of Mycobacterium smegmatis.
TextSentencer_T3 207-310 Sentence denotes At the final stage of purification, the enzyme preparation showed one major band of 59 kDa on SDS gels.
T3 207-310 Sentence denotes At the final stage of purification, the enzyme preparation showed one major band of 59 kDa on SDS gels.
T3 207-310 Sentence denotes At the final stage of purification, the enzyme preparation showed one major band of 59 kDa on SDS gels.
TextSentencer_T4 311-483 Sentence denotes The 59 kDa band became labeled with N3-UDP[32P]-glucose, and this labeling was inhibited in a concentration-dependent manner by either unlabeled UDP-glucose or GDP-glucose.
T4 311-483 Sentence denotes The 59 kDa band became labeled with N3-UDP[32P]-glucose, and this labeling was inhibited in a concentration-dependent manner by either unlabeled UDP-glucose or GDP-glucose.
T4 311-483 Sentence denotes The 59 kDa band became labeled with N3-UDP[32P]-glucose, and this labeling was inhibited in a concentration-dependent manner by either unlabeled UDP-glucose or GDP-glucose.
TextSentencer_T5 484-612 Sentence denotes The native enzyme also had a molecular weight of about 60 kDa by gel filtration, indicating that the active enzyme is a monomer.
T5 484-612 Sentence denotes The native enzyme also had a molecular weight of about 60 kDa by gel filtration, indicating that the active enzyme is a monomer.
T5 484-612 Sentence denotes The native enzyme also had a molecular weight of about 60 kDa by gel filtration, indicating that the active enzyme is a monomer.
TextSentencer_T6 613-732 Sentence denotes The 59 kDa protein was subjected to endoproteinase Lys-C digestion, and three peptides isolated by HPLC were sequenced.
T6 613-732 Sentence denotes The 59 kDa protein was subjected to endoproteinase Lys-C digestion, and three peptides isolated by HPLC were sequenced.
T6 613-732 Sentence denotes The 59 kDa protein was subjected to endoproteinase Lys-C digestion, and three peptides isolated by HPLC were sequenced.
TextSentencer_T7 733-896 Sentence denotes The sequences of 56 amino acids in these three peptides showed 60% identity to the trehalose-P synthases of Saccharomyces cerevesiae and Schizosaccharomyces pombe.
T7 733-896 Sentence denotes The sequences of 56 amino acids in these three peptides showed 60% identity to the trehalose-P synthases of Saccharomyces cerevesiae and Schizosaccharomyces pombe.
T7 733-896 Sentence denotes The sequences of 56 amino acids in these three peptides showed 60% identity to the trehalose-P synthases of Saccharomyces cerevesiae and Schizosaccharomyces pombe.
TextSentencer_T8 897-1103 Sentence denotes The purified mycobacterial enzyme catalyzed the synthesis of trehalose-P from glucose-6-P and a variety of nucleoside diphosphate glucose derivatives, depending on whether a polyanion was absent or present.
T8 897-1103 Sentence denotes The purified mycobacterial enzyme catalyzed the synthesis of trehalose-P from glucose-6-P and a variety of nucleoside diphosphate glucose derivatives, depending on whether a polyanion was absent or present.
T8 897-1103 Sentence denotes The purified mycobacterial enzyme catalyzed the synthesis of trehalose-P from glucose-6-P and a variety of nucleoside diphosphate glucose derivatives, depending on whether a polyanion was absent or present.
TextSentencer_T9 1104-1335 Sentence denotes Thus, UDP-glucose and GDP-glucose were the best glucosyl donors, but maximum activity with UDP-glucose required the presence of a polyanion such as heparin, whereas activity with GDP-glucose was relatively independent of polyanion.
T9 1104-1335 Sentence denotes Thus, UDP-glucose and GDP-glucose were the best glucosyl donors, but maximum activity with UDP-glucose required the presence of a polyanion such as heparin, whereas activity with GDP-glucose was relatively independent of polyanion.
T9 1104-1335 Sentence denotes Thus, UDP-glucose and GDP-glucose were the best glucosyl donors, but maximum activity with UDP-glucose required the presence of a polyanion such as heparin, whereas activity with GDP-glucose was relatively independent of polyanion.
TextSentencer_T10 1336-1467 Sentence denotes The presence of heparin in the incubation mixture increased the affinity of the enzyme for UDP-glucose by a factor of 100, or more.
T10 1336-1467 Sentence denotes The presence of heparin in the incubation mixture increased the affinity of the enzyme for UDP-glucose by a factor of 100, or more.
T10 1336-1467 Sentence denotes The presence of heparin in the incubation mixture increased the affinity of the enzyme for UDP-glucose by a factor of 100, or more.
TextSentencer_T11 1468-1557 Sentence denotes However, the affinity for GDP-glucose was only twofold better in the presence of heparin.
T11 1468-1557 Sentence denotes However, the affinity for GDP-glucose was only twofold better in the presence of heparin.
T11 1468-1557 Sentence denotes However, the affinity for GDP-glucose was only twofold better in the presence of heparin.
TextSentencer_T12 1558-1711 Sentence denotes The purified synthase also utilized ADP-glucose and CDP-glucose, but the K(m) for these glucosyl donors was quite high even in the presence of polyanion.
T12 1558-1711 Sentence denotes The purified synthase also utilized ADP-glucose and CDP-glucose, but the K(m) for these glucosyl donors was quite high even in the presence of polyanion.
T12 1558-1711 Sentence denotes The purified synthase also utilized ADP-glucose and CDP-glucose, but the K(m) for these glucosyl donors was quite high even in the presence of polyanion.
TextSentencer_T13 1712-1835 Sentence denotes The effect of heparin on UDP-glucose activity was dose-dependent and maximum at about 1-2 micrograms of heparin/incubation.
T13 1712-1835 Sentence denotes The effect of heparin on UDP-glucose activity was dose-dependent and maximum at about 1-2 micrograms of heparin/incubation.
T13 1712-1835 Sentence denotes The effect of heparin on UDP-glucose activity was dose-dependent and maximum at about 1-2 micrograms of heparin/incubation.
TextSentencer_T14 1836-2053 Sentence denotes However, the size of the heparin molecule (i.e., the number of monosaccharide residues) was critical for activation, and only those heparins with 18 or more monosaccharide units were effective in stimulating activity.
T14 1836-2053 Sentence denotes However, the size of the heparin molecule (i.e., the number of monosaccharide residues) was critical for activation, and only those heparins with 18 or more monosaccharide units were effective in stimulating activity.
T14 1836-2053 Sentence denotes However, the size of the heparin molecule (i.e., the number of monosaccharide residues) was critical for activation, and only those heparins with 18 or more monosaccharide units were effective in stimulating activity.