| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-137 |
Sentence |
denotes |
Polysialic acid export in Escherichia coli K1: the role of KpsT, the ATP-binding component of an ABC transporter, in chain translocation. |
| T1 |
0-137 |
Sentence |
denotes |
Polysialic acid export in Escherichia coli K1: the role of KpsT, the ATP-binding component of an ABC transporter, in chain translocation. |
| T1 |
0-137 |
Sentence |
denotes |
Polysialic acid export in Escherichia coli K1: the role of KpsT, the ATP-binding component of an ABC transporter, in chain translocation. |
| TextSentencer_T2 |
138-282 |
Sentence |
denotes |
The polysialic acid (polySia) capsule of Escherichia coli K1 is a key virulence determinant of the organism, allowing it to evade host defenses. |
| T2 |
138-282 |
Sentence |
denotes |
The polysialic acid (polySia) capsule of Escherichia coli K1 is a key virulence determinant of the organism, allowing it to evade host defenses. |
| T2 |
138-282 |
Sentence |
denotes |
The polysialic acid (polySia) capsule of Escherichia coli K1 is a key virulence determinant of the organism, allowing it to evade host defenses. |
| TextSentencer_T3 |
283-378 |
Sentence |
denotes |
The proteins necessary for expression of the capsule are encoded by the 17 kb kps gene cluster. |
| T3 |
283-378 |
Sentence |
denotes |
The proteins necessary for expression of the capsule are encoded by the 17 kb kps gene cluster. |
| T3 |
283-378 |
Sentence |
denotes |
The proteins necessary for expression of the capsule are encoded by the 17 kb kps gene cluster. |
| TextSentencer_T4 |
379-499 |
Sentence |
denotes |
This cluster contains two genes, kpsM and kpsT, that are required for polySia transport across the cytoplasmic membrane. |
| T4 |
379-499 |
Sentence |
denotes |
This cluster contains two genes, kpsM and kpsT, that are required for polySia transport across the cytoplasmic membrane. |
| T4 |
379-499 |
Sentence |
denotes |
This cluster contains two genes, kpsM and kpsT, that are required for polySia transport across the cytoplasmic membrane. |
| TextSentencer_T5 |
500-620 |
Sentence |
denotes |
KpsM is a hydrophobic integral inner membrane protein, while KpsT is a peripheral inner membrane protein that binds ATP. |
| T5 |
500-620 |
Sentence |
denotes |
KpsM is a hydrophobic integral inner membrane protein, while KpsT is a peripheral inner membrane protein that binds ATP. |
| T5 |
500-620 |
Sentence |
denotes |
KpsM is a hydrophobic integral inner membrane protein, while KpsT is a peripheral inner membrane protein that binds ATP. |
| TextSentencer_T6 |
621-695 |
Sentence |
denotes |
They belong to the ATP-binding cassette (ABC) superfamily of transporters. |
| T6 |
621-695 |
Sentence |
denotes |
They belong to the ATP-binding cassette (ABC) superfamily of transporters. |
| T6 |
621-695 |
Sentence |
denotes |
They belong to the ATP-binding cassette (ABC) superfamily of transporters. |
| TextSentencer_T7 |
696-835 |
Sentence |
denotes |
To study the role of KpsT in polySia translocation, we used PCR mutagenesis to isolate dominant negative mutations of plasmid-encoded kpsT. |
| T7 |
696-835 |
Sentence |
denotes |
To study the role of KpsT in polySia translocation, we used PCR mutagenesis to isolate dominant negative mutations of plasmid-encoded kpsT. |
| T7 |
696-835 |
Sentence |
denotes |
To study the role of KpsT in polySia translocation, we used PCR mutagenesis to isolate dominant negative mutations of plasmid-encoded kpsT. |
| TextSentencer_T8 |
836-943 |
Sentence |
denotes |
All mutations mapped to the same glutamic acid residue at position 150, adjacent to Walker motif B of KpsT. |
| T8 |
836-943 |
Sentence |
denotes |
All mutations mapped to the same glutamic acid residue at position 150, adjacent to Walker motif B of KpsT. |
| T8 |
836-943 |
Sentence |
denotes |
All mutations mapped to the same glutamic acid residue at position 150, adjacent to Walker motif B of KpsT. |
| TextSentencer_T9 |
944-1165 |
Sentence |
denotes |
Wild-type (kps+) cells harboring one such allele, E150G, did not transport polySia to the cell surface but accumulated intracellular polysaccharide and produced small colonies containing cells that grew as long filaments. |
| T9 |
944-1165 |
Sentence |
denotes |
Wild-type (kps+) cells harboring one such allele, E150G, did not transport polySia to the cell surface but accumulated intracellular polysaccharide and produced small colonies containing cells that grew as long filaments. |
| T9 |
944-1165 |
Sentence |
denotes |
Wild-type (kps+) cells harboring one such allele, E150G, did not transport polySia to the cell surface but accumulated intracellular polysaccharide and produced small colonies containing cells that grew as long filaments. |
| TextSentencer_T10 |
1166-1244 |
Sentence |
denotes |
The E150G protein still bound ATP as shown by 8-azidoATP photolabeling assays. |
| T10 |
1166-1244 |
Sentence |
denotes |
The E150G protein still bound ATP as shown by 8-azidoATP photolabeling assays. |
| T10 |
1166-1244 |
Sentence |
denotes |
The E150G protein still bound ATP as shown by 8-azidoATP photolabeling assays. |
| TextSentencer_T11 |
1245-1330 |
Sentence |
denotes |
We combined the E150G allele with each of five mutations isolated previously in kpsT. |
| T11 |
1245-1330 |
Sentence |
denotes |
We combined the E150G allele with each of five mutations isolated previously in kpsT. |
| T11 |
1245-1330 |
Sentence |
denotes |
We combined the E150G allele with each of five mutations isolated previously in kpsT. |
| TextSentencer_T12 |
1331-1581 |
Sentence |
denotes |
Mutations that disrupt ATP-binding (K44E) or alter regions of the protein thought to interact with KpsM (G84D, S126F) suppressed the dominant negative phenotype while mutations in the C-terminal portion of the protein (C163Y, H181Y) did not suppress. |
| T12 |
1331-1581 |
Sentence |
denotes |
Mutations that disrupt ATP-binding (K44E) or alter regions of the protein thought to interact with KpsM (G84D, S126F) suppressed the dominant negative phenotype while mutations in the C-terminal portion of the protein (C163Y, H181Y) did not suppress. |
| T12 |
1331-1581 |
Sentence |
denotes |
Mutations that disrupt ATP-binding (K44E) or alter regions of the protein thought to interact with KpsM (G84D, S126F) suppressed the dominant negative phenotype while mutations in the C-terminal portion of the protein (C163Y, H181Y) did not suppress. |
| TextSentencer_T13 |
1582-1696 |
Sentence |
denotes |
These studies have allowed the development of a working model for the role of KpsT in polySia chain translocation. |
| T13 |
1582-1696 |
Sentence |
denotes |
These studies have allowed the development of a working model for the role of KpsT in polySia chain translocation. |
| T13 |
1582-1696 |
Sentence |
denotes |
These studies have allowed the development of a working model for the role of KpsT in polySia chain translocation. |