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PubMed:8724132 JSONTXT 70 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-145 Sentence denotes Renaturation and ligand blotting of the major subunit of the rat asialoglycoprotein receptor after denaturing polyacrylamide gel electrophoresis.
T1 0-145 Sentence denotes Renaturation and ligand blotting of the major subunit of the rat asialoglycoprotein receptor after denaturing polyacrylamide gel electrophoresis.
TextSentencer_T2 146-286 Sentence denotes Rat hepatic asialoglycoprotein receptors (ASGP-Rs) bind terminal clustered galactosyl or N-acetylgalactosaminyl residues with high affinity.
T2 146-286 Sentence denotes Rat hepatic asialoglycoprotein receptors (ASGP-Rs) bind terminal clustered galactosyl or N-acetylgalactosaminyl residues with high affinity.
TextSentencer_T3 287-375 Sentence denotes The affinity-purified ASGP-R consists of three subunits designated RHL1, RHL2, and RHL3.
T3 287-375 Sentence denotes The affinity-purified ASGP-R consists of three subunits designated RHL1, RHL2, and RHL3.
TextSentencer_T4 376-621 Sentence denotes The ligand-binding activity of individual subunits was investigated by ligand blotting, after separation of subunits by SDS-PAGE under nonreducing conditions, electrotransfer to nitrocellulose, and incubation with 125I-asialo-orosomucoid (ASOR).
T4 376-621 Sentence denotes The ligand-binding activity of individual subunits was investigated by ligand blotting, after separation of subunits by SDS-PAGE under nonreducing conditions, electrotransfer to nitrocellulose, and incubation with 125I-asialo-orosomucoid (ASOR).
TextSentencer_T5 622-768 Sentence denotes No ligand-binding to any subunits could be detected when proteins such as BSA, casein, gelatin, or fat-free dry milk were used as blocking agents.
T5 622-768 Sentence denotes No ligand-binding to any subunits could be detected when proteins such as BSA, casein, gelatin, or fat-free dry milk were used as blocking agents.
TextSentencer_T6 769-899 Sentence denotes However, subsequent incubation of BSA-blocked nitrocellulose blots with some nonionic detergents resulted in renaturation of RHL1.
T6 769-899 Sentence denotes However, subsequent incubation of BSA-blocked nitrocellulose blots with some nonionic detergents resulted in renaturation of RHL1.
TextSentencer_T7 900-994 Sentence denotes 125I-ASOR-binding to RHL2 or RHL3 was weaker and could be detected only after longer exposure.
T7 900-994 Sentence denotes 125I-ASOR-binding to RHL2 or RHL3 was weaker and could be detected only after longer exposure.
TextSentencer_T8 995-1147 Sentence denotes Similarly, direct use of detergents such as Tween 20, Nonidet P-40, or Triton X-100 as blocking agents also preserved the ASOR-binding activity of RHL1.
T8 995-1147 Sentence denotes Similarly, direct use of detergents such as Tween 20, Nonidet P-40, or Triton X-100 as blocking agents also preserved the ASOR-binding activity of RHL1.
TextSentencer_T9 1148-1253 Sentence denotes Ionic detergents tested did not show any ability to renature the ligand-binding activity of RHL subunits.
T9 1148-1253 Sentence denotes Ionic detergents tested did not show any ability to renature the ligand-binding activity of RHL subunits.
TextSentencer_T10 1254-1478 Sentence denotes Among nonionic detergents tested, Tween 20, Tween 85, Lubrol PX, Nonidet P-40, and Triton X-100 were more effective than Tween 40, Tween 65, Tween 80, or Brij 35, whereas SPAN, digitonin, or octyl-glucoside showed no effect.
T10 1254-1478 Sentence denotes Among nonionic detergents tested, Tween 20, Tween 85, Lubrol PX, Nonidet P-40, and Triton X-100 were more effective than Tween 40, Tween 65, Tween 80, or Brij 35, whereas SPAN, digitonin, or octyl-glucoside showed no effect.
TextSentencer_T11 1479-1586 Sentence denotes Weak 125I-ASOR binding to RHL2 or RHL3 could be detected only when the Tween series or Lubrol PX were used.
T11 1479-1586 Sentence denotes Weak 125I-ASOR binding to RHL2 or RHL3 could be detected only when the Tween series or Lubrol PX were used.
TextSentencer_T12 1587-1676 Sentence denotes Incubation of blots with dithiothreitol caused a dose-dependent loss of binding activity.
T12 1587-1676 Sentence denotes Incubation of blots with dithiothreitol caused a dose-dependent loss of binding activity.
TextSentencer_T13 1677-1900 Sentence denotes The carbohydrate recognition domain (CRD) of RHL1, isolated after subtilisin digestion of ASGP-R bound to ASOR-Sepharose, retained ligand-binding activity as assessed by its binding to ASOR-Sepharose and by ligand blotting.
T13 1677-1900 Sentence denotes The carbohydrate recognition domain (CRD) of RHL1, isolated after subtilisin digestion of ASGP-R bound to ASOR-Sepharose, retained ligand-binding activity as assessed by its binding to ASOR-Sepharose and by ligand blotting.
TextSentencer_T14 1901-2014 Sentence denotes 125I-ASOR binding to electroblotted CRD after SDS-PAGE was also dependent on the presence of nonionic detergents.
T14 1901-2014 Sentence denotes 125I-ASOR binding to electroblotted CRD after SDS-PAGE was also dependent on the presence of nonionic detergents.
TextSentencer_T15 2015-2223 Sentence denotes We conclude that restoration of ligand-binding activity of RHL1 after SDS-PAGE by some nonionic detergents is not dependent on the presence of the cytoplasmic, transmembrane, or stalk domains of this subunit.
T15 2015-2223 Sentence denotes We conclude that restoration of ligand-binding activity of RHL1 after SDS-PAGE by some nonionic detergents is not dependent on the presence of the cytoplasmic, transmembrane, or stalk domains of this subunit.