> top > projects > sentences > docs > PubMed:8702527 > annotations
sentences Home  

PubMed:8702527 JSONTXT 29 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-148 Sentence denotes An insulin receptor mutant (Asp707 --> Ala), involved in leprechaunism, is processed and transported to the cell surface but unable to bind insulin.
T1 0-148 Sentence denotes An insulin receptor mutant (Asp707 --> Ala), involved in leprechaunism, is processed and transported to the cell surface but unable to bind insulin.
TextSentencer_T2 149-312 Sentence denotes We have identified a homozygous mutation near the carboxyl terminus of the insulin receptor (IR) alpha subunit from a leprechaun patient, changing Asp707 into Ala.
T2 149-312 Sentence denotes We have identified a homozygous mutation near the carboxyl terminus of the insulin receptor (IR) alpha subunit from a leprechaun patient, changing Asp707 into Ala.
TextSentencer_T3 313-386 Sentence denotes Fibroblasts from this patient had no high affinity insulin binding sites.
T3 313-386 Sentence denotes Fibroblasts from this patient had no high affinity insulin binding sites.
TextSentencer_T4 387-509 Sentence denotes To examine the effect of the mutation on IR properties, the mutant IR was stably expressed in Chinese hamster ovary cells.
T4 387-509 Sentence denotes To examine the effect of the mutation on IR properties, the mutant IR was stably expressed in Chinese hamster ovary cells.
TextSentencer_T5 510-632 Sentence denotes Western blot analysis and metabolic labeling showed a normal processing of the mutant receptor to alpha and beta subunits.
T5 510-632 Sentence denotes Western blot analysis and metabolic labeling showed a normal processing of the mutant receptor to alpha and beta subunits.
TextSentencer_T6 633-900 Sentence denotes No increase in high affinity insulin binding sites was observed on Chinese hamster ovary cells expressing the mutant receptor, and also, affinity cross-linking of 125I-labeled insulin by disuccinimidyl suberate to these cells failed to label the mutant alpha subunit.
T6 633-900 Sentence denotes No increase in high affinity insulin binding sites was observed on Chinese hamster ovary cells expressing the mutant receptor, and also, affinity cross-linking of 125I-labeled insulin by disuccinimidyl suberate to these cells failed to label the mutant alpha subunit.
TextSentencer_T7 901-1088 Sentence denotes Biotinylation of cell surface proteins by biotin succinimidyl ester resulted in efficient biotinylation of the mutant IR alpha and beta subunits, showing its presence on the cell surface.
T7 901-1088 Sentence denotes Biotinylation of cell surface proteins by biotin succinimidyl ester resulted in efficient biotinylation of the mutant IR alpha and beta subunits, showing its presence on the cell surface.
TextSentencer_T8 1089-1273 Sentence denotes On solubilization of the mutant insulin receptor in Triton X-100-containing buffers, 125I-insulin was efficiently cross-linked to the receptor alpha subunit by disuccinimidyl suberate.
T8 1089-1273 Sentence denotes On solubilization of the mutant insulin receptor in Triton X-100-containing buffers, 125I-insulin was efficiently cross-linked to the receptor alpha subunit by disuccinimidyl suberate.
TextSentencer_T9 1274-1429 Sentence denotes These studies demonstrate that Ala707 IR is normally processed and transported to the cell surface and that the mutation distorts the insulin binding site.
T9 1274-1429 Sentence denotes These studies demonstrate that Ala707 IR is normally processed and transported to the cell surface and that the mutation distorts the insulin binding site.
TextSentencer_T10 1430-1459 Sentence denotes Detergent restores this site.
T10 1430-1459 Sentence denotes Detergent restores this site.
TextSentencer_T11 1460-1618 Sentence denotes This is an example of a naturally occurring mutation in the insulin receptor that affects insulin binding without affecting receptor transport and processing.
T11 1460-1618 Sentence denotes This is an example of a naturally occurring mutation in the insulin receptor that affects insulin binding without affecting receptor transport and processing.
TextSentencer_T12 1619-1722 Sentence denotes This mutation points to a major contribution of the alpha subunit carboxyl terminus to insulin binding.
T12 1619-1722 Sentence denotes This mutation points to a major contribution of the alpha subunit carboxyl terminus to insulin binding.