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Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-120 Sentence denotes Purification and characterization of calmodulin-dependent protein kinase III from rabbit reticulocytes and rat pancreas.
T1 0-120 Sentence denotes Purification and characterization of calmodulin-dependent protein kinase III from rabbit reticulocytes and rat pancreas.
T2 121-255 Sentence denotes Calmodulin-dependent protein kinase III (CaM kinase III) phosphorylates and thereby inactivates eukaryotic elongation factor-2 (EF-2).
T2 121-255 Sentence denotes Calmodulin-dependent protein kinase III (CaM kinase III) phosphorylates and thereby inactivates eukaryotic elongation factor-2 (EF-2).
T3 256-609 Sentence denotes This enzyme, purified to homogeneity from either rabbit reticulocytes or rat pancreas, had similar properties: it migrated with an apparent M(r) of 140,000 by gel filtration, was comprised of a major polypeptide of M(r) 95,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and had a high affinity for CaM (half-maximal activation < 1 nM).
T3 256-609 Sentence denotes This enzyme, purified to homogeneity from either rabbit reticulocytes or rat pancreas, had similar properties: it migrated with an apparent M(r) of 140,000 by gel filtration, was comprised of a major polypeptide of M(r) 95,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and had a high affinity for CaM (half-maximal activation < 1 nM).
T4 610-825 Sentence denotes The M(r) 95,000 polypeptide was autophosphorylated by an intramolecular mechanism on seryl residues in the presence of Ca2+, CaM, and ATP, and phosphopeptide mapping indicated that several sites were phosphorylated.
T4 610-825 Sentence denotes The M(r) 95,000 polypeptide was autophosphorylated by an intramolecular mechanism on seryl residues in the presence of Ca2+, CaM, and ATP, and phosphopeptide mapping indicated that several sites were phosphorylated.
T5 826-943 Sentence denotes Autophosphorylation of CaM kinase III resulted in the generation of a partially Ca2+/calmodulin-independent activity.
T5 826-943 Sentence denotes Autophosphorylation of CaM kinase III resulted in the generation of a partially Ca2+/calmodulin-independent activity.
T6 944-1042 Sentence denotes The enzyme could also be phosphorylated by the catalytic subunit of cAMP-dependent protein kinase.
T6 944-1042 Sentence denotes The enzyme could also be phosphorylated by the catalytic subunit of cAMP-dependent protein kinase.
T7 1043-1318 Sentence denotes Amino acid sequencing of CaM kinase III indicated that it is distinct from other known proteins including the heat-shock protein hsp90, which was recently suggested to be identical to CaM kinase III (Nygärd, O., Nilsson, A., Carlberg, U., Nilsson, L., and Amons, R. (1991) J.
T7 1043-1318 Sentence denotes Amino acid sequencing of CaM kinase III indicated that it is distinct from other known proteins including the heat-shock protein hsp90, which was recently suggested to be identical to CaM kinase III (Nygärd, O., Nilsson, A., Carlberg, U., Nilsson, L., and Amons, R. (1991) J.
T8 1319-1324 Sentence denotes Biol.
T8 1319-1324 Sentence denotes Biol.
T9 1325-1330 Sentence denotes Chem.
T9 1325-1330 Sentence denotes Chem.
T10 1331-1349 Sentence denotes 266, 16425-16430).
T10 1331-1349 Sentence denotes 266, 16425-16430).
T11 1350-1480 Sentence denotes Furthermore, hsp90 did not copurify with CaM kinase III, and the M(r) 95,000 protein did not cross-react with antibodies to hsp90.
T11 1350-1480 Sentence denotes Furthermore, hsp90 did not copurify with CaM kinase III, and the M(r) 95,000 protein did not cross-react with antibodies to hsp90.
T12 1481-1616 Sentence denotes CaM kinase III exhibited Michaelis-Menten kinetics toward its substrates ATP and EF-2, with Km values of 15 and 2 microM, respectively.
T12 1481-1616 Sentence denotes CaM kinase III exhibited Michaelis-Menten kinetics toward its substrates ATP and EF-2, with Km values of 15 and 2 microM, respectively.
T13 1617-1831 Sentence denotes CaM kinase III was able to phosphorylate yeast EF-2 with an Km of 2 microM, but the enzyme did not significantly phosphorylate a variety of other substrates, confirming its identification as a novel protein kinase.
T13 1617-1831 Sentence denotes CaM kinase III was able to phosphorylate yeast EF-2 with an Km of 2 microM, but the enzyme did not significantly phosphorylate a variety of other substrates, confirming its identification as a novel protein kinase.