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Id Subject Object Predicate Lexical cue
T1 0-131 Sentence denotes Kinetic and spatial interrelationships between ganglioside glycosyltransferases and O-acetyltransferase(s) in human melanoma cells.
T1 0-131 Sentence denotes Kinetic and spatial interrelationships between ganglioside glycosyltransferases and O-acetyltransferase(s) in human melanoma cells.
T2 132-258 Sentence denotes The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized.
T2 132-258 Sentence denotes The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized.
T3 259-407 Sentence denotes However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure.
T3 259-407 Sentence denotes However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure.
T4 408-617 Sentence denotes Here, we have studied the spatial and temporal interrelationships among the activities responsible for the later stages of ganglioside biosynthesis and those for O-acetylation in cultured human melanoma cells.
T4 408-617 Sentence denotes Here, we have studied the spatial and temporal interrelationships among the activities responsible for the later stages of ganglioside biosynthesis and those for O-acetylation in cultured human melanoma cells.
T5 618-767 Sentence denotes First, brefeldin A treatment was used to separate biosynthetic steps into compartments distal or proximal to the transport block imposed by the drug.
T5 618-767 Sentence denotes First, brefeldin A treatment was used to separate biosynthetic steps into compartments distal or proximal to the transport block imposed by the drug.
T6 768-884 Sentence denotes In keeping with prior reports, GM2/GD2 synthase was consistently rendered inaccessible to its acceptors GM3 and GD3.
T6 768-884 Sentence denotes In keeping with prior reports, GM2/GD2 synthase was consistently rendered inaccessible to its acceptors GM3 and GD3.
T7 885-952 Sentence denotes In contrast, the effect on GD3 biosynthesis was cell line-specific.
T7 885-952 Sentence denotes In contrast, the effect on GD3 biosynthesis was cell line-specific.
T8 953-1042 Sentence denotes Synthesis of GD3 was nearly abrogated in two lines, while it accumulated in a third line.
T8 953-1042 Sentence denotes Synthesis of GD3 was nearly abrogated in two lines, while it accumulated in a third line.
T9 1043-1166 Sentence denotes This indicates that the spatial organization of ganglioside processing activities can vary even between similar cell lines.
T9 1043-1166 Sentence denotes This indicates that the spatial organization of ganglioside processing activities can vary even between similar cell lines.
T10 1167-1429 Sentence denotes However, in all cell lines studied, the ratio of 9(7)-O-acetyl-GD3 to GD3 was not changed by brefeldin A, indicating that the majority of ganglioside O-acetyltransferase activity is co-localized with GD3 biosynthetic activity in the same Golgi subcompartment(s).
T10 1167-1429 Sentence denotes However, in all cell lines studied, the ratio of 9(7)-O-acetyl-GD3 to GD3 was not changed by brefeldin A, indicating that the majority of ganglioside O-acetyltransferase activity is co-localized with GD3 biosynthetic activity in the same Golgi subcompartment(s).
T11 1430-1583 Sentence denotes As an alternative approach, Golgi-enriched fractions from melanoma cells were incubated with radiolabeled and nonlabeled nucleotide sugars or acetyl-CoA.
T11 1430-1583 Sentence denotes As an alternative approach, Golgi-enriched fractions from melanoma cells were incubated with radiolabeled and nonlabeled nucleotide sugars or acetyl-CoA.
T12 1584-1819 Sentence denotes In these preparations, biosynthesis is dependent upon the co-localization of appropriate sugar nucleotide transporters, glycosyltransferases, and acceptors that are endogenously present within intact topologically correct compartments.
T12 1584-1819 Sentence denotes In these preparations, biosynthesis is dependent upon the co-localization of appropriate sugar nucleotide transporters, glycosyltransferases, and acceptors that are endogenously present within intact topologically correct compartments.
T13 1820-2012 Sentence denotes Incubations with CMP-Neu5Ac and acetyl-CoA corroborated the results with brefeldin A, co-localizing ganglioside O-acetyltransferase activity in compartments where GD3 biosynthesis takes place.
T13 1820-2012 Sentence denotes Incubations with CMP-Neu5Ac and acetyl-CoA corroborated the results with brefeldin A, co-localizing ganglioside O-acetyltransferase activity in compartments where GD3 biosynthesis takes place.
T14 2013-2131 Sentence denotes Analyses with CMP-Neu5Ac and UDP-GalNAc showed that GD2 and GD3 synthesis occur in partially overlapping compartments.
T14 2013-2131 Sentence denotes Analyses with CMP-Neu5Ac and UDP-GalNAc showed that GD2 and GD3 synthesis occur in partially overlapping compartments.
T15 2132-2381 Sentence denotes Labeling with acetyl-CoA and UDP-GalNAc indicated that although labeled acetate can be transferred from acetyl-CoA directly to GD2, ganglioside O-acetyltransferase activity does not substantially overlap with the biosynthetic compartment(s) for GD2.
T15 2132-2381 Sentence denotes Labeling with acetyl-CoA and UDP-GalNAc indicated that although labeled acetate can be transferred from acetyl-CoA directly to GD2, ganglioside O-acetyltransferase activity does not substantially overlap with the biosynthetic compartment(s) for GD2.
T16 2382-2515 Sentence denotes Instead, O-acetyl-GD3 appears to be co-localized with the compartment of GD2 biosynthesis and serves as an acceptor for GD2 synthase.
T16 2382-2515 Sentence denotes Instead, O-acetyl-GD3 appears to be co-localized with the compartment of GD2 biosynthesis and serves as an acceptor for GD2 synthase.
T17 2516-2627 Sentence denotes Thus, both 9-O-acetyl-GD3 and GD2 can be precursors of 9-O-acetyl-GD2, but apparently in distinct compartments.
T17 2516-2627 Sentence denotes Thus, both 9-O-acetyl-GD3 and GD2 can be precursors of 9-O-acetyl-GD2, but apparently in distinct compartments.