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PubMed:8440713 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes Identification and purification of a calcium-binding protein in hepatic nuclear membranes.
T1 0-90 Sentence denotes Identification and purification of a calcium-binding protein in hepatic nuclear membranes.
T2 91-213 Sentence denotes Recent evidence suggests that nuclei possess Ca2+ transport mechanisms to regulate nucleoplasmic/cytosolic Ca2+ gradients.
T2 91-213 Sentence denotes Recent evidence suggests that nuclei possess Ca2+ transport mechanisms to regulate nucleoplasmic/cytosolic Ca2+ gradients.
T3 214-321 Sentence denotes We, therefore, investigated the possibility that Ca(2+)-binding proteins may also exist within the nucleus.
T3 214-321 Sentence denotes We, therefore, investigated the possibility that Ca(2+)-binding proteins may also exist within the nucleus.
T4 322-689 Sentence denotes Electrophoretic analysis revealed the presence of an acidic 93-kDa protein (p93) in the membranes of isolated nuclei. p93 stained blue with "Stains-All" in SDS-polyacrylamide gels and was the major 45Ca(2+)- and ruthenium red-binding nuclear envelope protein in electroblot overlays. p93 was resistant to extraction by 6 M urea but was solubilized in 2% Triton X-100.
T4 322-689 Sentence denotes Electrophoretic analysis revealed the presence of an acidic 93-kDa protein (p93) in the membranes of isolated nuclei. p93 stained blue with "Stains-All" in SDS-polyacrylamide gels and was the major 45Ca(2+)- and ruthenium red-binding nuclear envelope protein in electroblot overlays. p93 was resistant to extraction by 6 M urea but was solubilized in 2% Triton X-100.
T5 690-853 Sentence denotes Citric acid was highly effective in removing the outer nuclear membrane (ER) with concomitant reduction (< 10-fold) of mannose-6-phosphatase activity, but not p93.
T5 690-853 Sentence denotes Citric acid was highly effective in removing the outer nuclear membrane (ER) with concomitant reduction (< 10-fold) of mannose-6-phosphatase activity, but not p93.
T6 854-981 Sentence denotes 45Ca(2+)-binding assays of purified p93 revealed the presence of high capacity Ca(2+)-binding sites comparable to calreticulin.
T6 854-981 Sentence denotes 45Ca(2+)-binding assays of purified p93 revealed the presence of high capacity Ca(2+)-binding sites comparable to calreticulin.
T7 982-1096 Sentence denotes This evidence strongly suggests that p93 is a major Ca(2+)-binding protein of the inner nuclear envelope membrane.
T7 982-1096 Sentence denotes This evidence strongly suggests that p93 is a major Ca(2+)-binding protein of the inner nuclear envelope membrane.
T8 1097-1252 Sentence denotes Partial amino acid sequence analysis revealed that p93 was close to 100% homologous with a recently identified ER Ca(2+)-binding protein known as calnexin.
T8 1097-1252 Sentence denotes Partial amino acid sequence analysis revealed that p93 was close to 100% homologous with a recently identified ER Ca(2+)-binding protein known as calnexin.
T9 1253-1299 Sentence denotes It is likely, therefore, that p93 is calnexin.
T9 1253-1299 Sentence denotes It is likely, therefore, that p93 is calnexin.
T10 1300-1463 Sentence denotes However, mild CHAPS detergent treatment of nuclear envelopes and ER revealed distinctly different solubility properties of each membrane for the extraction of p93.
T10 1300-1463 Sentence denotes However, mild CHAPS detergent treatment of nuclear envelopes and ER revealed distinctly different solubility properties of each membrane for the extraction of p93.
T11 1464-1608 Sentence denotes This, together with the citrate data, strongly suggests that p93/calnexin, in isolated nuclear envelopes, is mostly bound to the inner membrane.
T11 1464-1608 Sentence denotes This, together with the citrate data, strongly suggests that p93/calnexin, in isolated nuclear envelopes, is mostly bound to the inner membrane.
T12 1609-1734 Sentence denotes It is possible that p93 may be involved with the regulation of Ca2+ transients between the nucleoplasm and perinuclear space.
T12 1609-1734 Sentence denotes It is possible that p93 may be involved with the regulation of Ca2+ transients between the nucleoplasm and perinuclear space.