| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-75 |
Sentence |
denotes |
Methylated nucleosides in globin mRNA from mouse nucleated erythroid cells. |
| T1 |
0-75 |
Sentence |
denotes |
Methylated nucleosides in globin mRNA from mouse nucleated erythroid cells. |
| T2 |
76-222 |
Sentence |
denotes |
Poly(A)-containing mRNAs labeled with [methyl-3H]methionine were isolated from nucleated erythroid cells obtained from the spleens of anemic mice. |
| T2 |
76-222 |
Sentence |
denotes |
Poly(A)-containing mRNAs labeled with [methyl-3H]methionine were isolated from nucleated erythroid cells obtained from the spleens of anemic mice. |
| T3 |
223-376 |
Sentence |
denotes |
The RNAs were further separated into non-globin poly(A)-containing RNAs and highly purified globin mRNA by globin cDNA-cellulose affinity chromatography. |
| T3 |
223-376 |
Sentence |
denotes |
The RNAs were further separated into non-globin poly(A)-containing RNAs and highly purified globin mRNA by globin cDNA-cellulose affinity chromatography. |
| T4 |
377-582 |
Sentence |
denotes |
DEAE-Sephadex column chromatography of the T2 ribonuclease digestion products of the cDNA-purified globin mRNA fraction yielded methylated resistant fragments with charges of -4.7 (Cap 1) and -5.3 (Cap 2). |
| T4 |
377-582 |
Sentence |
denotes |
DEAE-Sephadex column chromatography of the T2 ribonuclease digestion products of the cDNA-purified globin mRNA fraction yielded methylated resistant fragments with charges of -4.7 (Cap 1) and -5.3 (Cap 2). |
| T5 |
583-746 |
Sentence |
denotes |
Digestion of the non-globin RNA fraction revealed a similar pattern with the addition of a methylated mononucleotide identified as 6-methyladenosine at -2 charges. |
| T5 |
583-746 |
Sentence |
denotes |
Digestion of the non-globin RNA fraction revealed a similar pattern with the addition of a methylated mononucleotide identified as 6-methyladenosine at -2 charges. |
| T6 |
747-913 |
Sentence |
denotes |
Alkaline phosphatase treatment of the T2 resistant fragments reduced their charges by approximately 2, which is consistent with the removal of one terminal phosphate. |
| T6 |
747-913 |
Sentence |
denotes |
Alkaline phosphatase treatment of the T2 resistant fragments reduced their charges by approximately 2, which is consistent with the removal of one terminal phosphate. |
| T7 |
914-1095 |
Sentence |
denotes |
Treatment of the globin T2 and alkaline phosphatase-resistant fragments withpenicillium P1 nuclease and alkaline phosphatase yielded a P1-resistant core structure in both fragments. |
| T7 |
914-1095 |
Sentence |
denotes |
Treatment of the globin T2 and alkaline phosphatase-resistant fragments withpenicillium P1 nuclease and alkaline phosphatase yielded a P1-resistant core structure in both fragments. |
| T8 |
1096-1208 |
Sentence |
denotes |
In addition to the core, 2'-O-methylcytidine (Cm) was released from the more negatively charged globin fragment. |
| T8 |
1096-1208 |
Sentence |
denotes |
In addition to the core, 2'-O-methylcytidine (Cm) was released from the more negatively charged globin fragment. |
| T9 |
1209-1406 |
Sentence |
denotes |
The P1-resistant cores of the cap structures eluted from DEAE-Sephadex with the known standard m2G5'ppp5'Am and were found to be pyrophosphatase-sensitive establishing a 5'-5'-triphosphate linkage. |
| T9 |
1209-1406 |
Sentence |
denotes |
The P1-resistant cores of the cap structures eluted from DEAE-Sephadex with the known standard m2G5'ppp5'Am and were found to be pyrophosphatase-sensitive establishing a 5'-5'-triphosphate linkage. |
| T10 |
1407-1698 |
Sentence |
denotes |
The pyrophosphatase and alkaline phosphatase digestion products of the globin Cap 1 and Cap 2 core structures were analyzed by high voltage electrophoresis and paper chromatography and found to be 7-methyiguanosine (m7G) and the dimethylated nucleoside 6-methyl-2'-O-methyladenosine (N6mAm). |
| T10 |
1407-1698 |
Sentence |
denotes |
The pyrophosphatase and alkaline phosphatase digestion products of the globin Cap 1 and Cap 2 core structures were analyzed by high voltage electrophoresis and paper chromatography and found to be 7-methyiguanosine (m7G) and the dimethylated nucleoside 6-methyl-2'-O-methyladenosine (N6mAm). |
| T11 |
1699-1798 |
Sentence |
denotes |
A small amount of the singularly methylated adenosine, 2'-O-methyladenosine (Am) was also observed. |
| T11 |
1699-1798 |
Sentence |
denotes |
A small amount of the singularly methylated adenosine, 2'-O-methyladenosine (Am) was also observed. |
| T12 |
1799-1937 |
Sentence |
denotes |
The predominant sequences of the methylated nucleosides in the globin cap structures are therefore m7G5'ppp5'N6mAm and m7G5'ppp5'N6mAmpCm. |
| T12 |
1799-1937 |
Sentence |
denotes |
The predominant sequences of the methylated nucleosides in the globin cap structures are therefore m7G5'ppp5'N6mAm and m7G5'ppp5'N6mAmpCm. |