Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-88 |
Sentence |
denotes |
Separation of splicing factor SF3 into two components and purification of SF3a activity. |
T1 |
0-88 |
Sentence |
denotes |
Separation of splicing factor SF3 into two components and purification of SF3a activity. |
T2 |
89-206 |
Sentence |
denotes |
Components required for the splicing of nuclear messenger RNA precursors in vitro have been isolated from HeLa cells. |
T2 |
89-206 |
Sentence |
denotes |
Components required for the splicing of nuclear messenger RNA precursors in vitro have been isolated from HeLa cells. |
T3 |
207-297 |
Sentence |
denotes |
Here we describe the separation of splicing factor SF3 into two components, SF3a and SF3b. |
T3 |
207-297 |
Sentence |
denotes |
Here we describe the separation of splicing factor SF3 into two components, SF3a and SF3b. |
T4 |
298-548 |
Sentence |
denotes |
Both activities are required together with several other protein factors and U1 and U2 small nuclear ribonucleoproteins for the assembly of a presplicing complex which represents the first ATP-dependent step in the assembly of the active spliceosome. |
T4 |
298-548 |
Sentence |
denotes |
Both activities are required together with several other protein factors and U1 and U2 small nuclear ribonucleoproteins for the assembly of a presplicing complex which represents the first ATP-dependent step in the assembly of the active spliceosome. |
T5 |
549-688 |
Sentence |
denotes |
SF3a has been purified to homogeneity by a combination of ion-exchange chromatography, gel filtration, and glycerol gradient sedimentation. |
T5 |
549-688 |
Sentence |
denotes |
SF3a has been purified to homogeneity by a combination of ion-exchange chromatography, gel filtration, and glycerol gradient sedimentation. |
T6 |
689-759 |
Sentence |
denotes |
It consists of a complex of three polypeptides of 60, 66, and 120 kDa. |
T6 |
689-759 |
Sentence |
denotes |
It consists of a complex of three polypeptides of 60, 66, and 120 kDa. |
T7 |
760-949 |
Sentence |
denotes |
The association of SF3a activity with these polypeptides has been confirmed by immunoprecipitation and depletion experiments using a monoclonal antibody directed against the 66-kDa subunit. |
T7 |
760-949 |
Sentence |
denotes |
The association of SF3a activity with these polypeptides has been confirmed by immunoprecipitation and depletion experiments using a monoclonal antibody directed against the 66-kDa subunit. |