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Id Subject Object Predicate Lexical cue
T1 0-74 Sentence denotes Nuclear localization of glycosaminoglycans in rat ovarian granulosa cells.
T1 0-74 Sentence denotes Nuclear localization of glycosaminoglycans in rat ovarian granulosa cells.
T2 75-278 Sentence denotes In rat ovarian granulosa cell cultures, internalized cell surface heparan sulfate is processed by either a fast lysosome-mediated pathway or by a slow pathway which generates glycosaminoglycan fragments.
T2 75-278 Sentence denotes In rat ovarian granulosa cell cultures, internalized cell surface heparan sulfate is processed by either a fast lysosome-mediated pathway or by a slow pathway which generates glycosaminoglycan fragments.
T3 279-476 Sentence denotes Cell-associated dermatan sulfate proteoglycans also undergo processing through analogous pathways, although the slow pathway does not involve endoglycosidic cleavage of the dermatan sulfate chains.
T3 279-476 Sentence denotes Cell-associated dermatan sulfate proteoglycans also undergo processing through analogous pathways, although the slow pathway does not involve endoglycosidic cleavage of the dermatan sulfate chains.
T4 477-628 Sentence denotes In the present study we tested whether intracellular glycosaminoglycan fragments in rat ovarian granulosa cells were transient residents of the nuclei.
T4 477-628 Sentence denotes In the present study we tested whether intracellular glycosaminoglycan fragments in rat ovarian granulosa cells were transient residents of the nuclei.
T5 629-759 Sentence denotes A technique for isolating nuclei was devised in which cells were lysed with a hypo-osmotic extraction buffer containing detergent.
T5 629-759 Sentence denotes A technique for isolating nuclei was devised in which cells were lysed with a hypo-osmotic extraction buffer containing detergent.
T6 760-898 Sentence denotes Nuclei were then purified by conventional methods, and final preparations gave excellent recovery of the starting DNA (approximately 90%).
T6 760-898 Sentence denotes Nuclei were then purified by conventional methods, and final preparations gave excellent recovery of the starting DNA (approximately 90%).
T7 899-1026 Sentence denotes The technique was used to isolate glycosaminoglycans from nuclei after cells were metabolically radiolabeled with [35S]sulfate.
T7 899-1026 Sentence denotes The technique was used to isolate glycosaminoglycans from nuclei after cells were metabolically radiolabeled with [35S]sulfate.
T8 1027-1153 Sentence denotes The results indicated the possible presence of dermatan sulfate, but not heparan sulfate, glycosaminoglycans at this location.
T8 1027-1153 Sentence denotes The results indicated the possible presence of dermatan sulfate, but not heparan sulfate, glycosaminoglycans at this location.
T9 1154-1341 Sentence denotes Failure to remove cell surface proteoglycans with trypsin before preparation of nuclei resulted in nuclear contamination with significant amounts of intact dermatan sulfate proteoglycans.
T9 1154-1341 Sentence denotes Failure to remove cell surface proteoglycans with trypsin before preparation of nuclei resulted in nuclear contamination with significant amounts of intact dermatan sulfate proteoglycans.
T10 1342-1549 Sentence denotes Nuclei preparations obtained using traditional homogenization steps from cells either treated without or with trypsin gave identical results except that recoveries of DNA were much lower (approximately 30%).
T10 1342-1549 Sentence denotes Nuclei preparations obtained using traditional homogenization steps from cells either treated without or with trypsin gave identical results except that recoveries of DNA were much lower (approximately 30%).
T11 1550-1713 Sentence denotes The results demonstrate the difficulty in isolating pure nuclei and therefore also of firm conclusions pertaining to the nuclear association of glycosaminoglycans.
T11 1550-1713 Sentence denotes The results demonstrate the difficulty in isolating pure nuclei and therefore also of firm conclusions pertaining to the nuclear association of glycosaminoglycans.