> top > projects > sentences > docs > PubMed:8307953 > annotations
sentences Home  

PubMed:8307953 JSONTXT 20 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-114 Sentence denotes Heparin increases the affinity of basic fibroblast growth factor for its receptor but is not required for binding.
T1 0-114 Sentence denotes Heparin increases the affinity of basic fibroblast growth factor for its receptor but is not required for binding.
T2 115-562 Sentence denotes The role of heparin or heparan sulfates in the interaction of basic fibroblast growth factor (bFGF) with its high affinity receptor were investigated using purified extracellular ligand-binding region of FGF receptor-1 (FGFR-1) and intact receptors expressed in a myeloid cell line (32D) that does not express detectable levels of heparan sulfate proteoglycans or in Chinese hamster ovary (CHO) cell mutants defective in heparan sulfate synthesis.
T2 115-562 Sentence denotes The role of heparin or heparan sulfates in the interaction of basic fibroblast growth factor (bFGF) with its high affinity receptor were investigated using purified extracellular ligand-binding region of FGF receptor-1 (FGFR-1) and intact receptors expressed in a myeloid cell line (32D) that does not express detectable levels of heparan sulfate proteoglycans or in Chinese hamster ovary (CHO) cell mutants defective in heparan sulfate synthesis.
T3 563-682 Sentence denotes The purified extracellular domain of FGFR-1 formed complexes with 125I-bFGF both in the presence or absence of heparin.
T3 563-682 Sentence denotes The purified extracellular domain of FGFR-1 formed complexes with 125I-bFGF both in the presence or absence of heparin.
T4 683-847 Sentence denotes Intact FGFR-1 expressed in 32D cells also bound the same amount of 125I-bFGF in the presence or absence of heparin when saturating concentrations of bFGF were used.
T4 683-847 Sentence denotes Intact FGFR-1 expressed in 32D cells also bound the same amount of 125I-bFGF in the presence or absence of heparin when saturating concentrations of bFGF were used.
T5 848-1042 Sentence denotes Varying the concentration of 125I-bFGF showed that heparin increased the amount of 125I-bFGF bound at low bFGF concentrations and increased the affinity of bFGF for its receptor by about 3-fold.
T5 848-1042 Sentence denotes Varying the concentration of 125I-bFGF showed that heparin increased the amount of 125I-bFGF bound at low bFGF concentrations and increased the affinity of bFGF for its receptor by about 3-fold.
T6 1043-1184 Sentence denotes To eliminate the possibility of alteration of bFGF properties through the chemical modification reactions, bFGF was labeled biosynthetically.
T6 1043-1184 Sentence denotes To eliminate the possibility of alteration of bFGF properties through the chemical modification reactions, bFGF was labeled biosynthetically.
T7 1185-1269 Sentence denotes The binding of biosynthetically labeled bFGF to FGFR-1 also did not require heparin.
T7 1185-1269 Sentence denotes The binding of biosynthetically labeled bFGF to FGFR-1 also did not require heparin.
T8 1270-1511 Sentence denotes When FGFR-1 or FGFR-2 were expressed in mutant CHO cells deficient in heparan sulfate synthesis, the cells also bound 125I-bFGF in the absence of heparin, and the addition of heparin increased the affinity of bFGF for its receptors 2-3-fold.
T8 1270-1511 Sentence denotes When FGFR-1 or FGFR-2 were expressed in mutant CHO cells deficient in heparan sulfate synthesis, the cells also bound 125I-bFGF in the absence of heparin, and the addition of heparin increased the affinity of bFGF for its receptors 2-3-fold.
T9 1512-1658 Sentence denotes Thus, heparin or heparan sulfate is not required for the binding of bFGF to its receptors but increases the binding affinity to a moderate degree.
T9 1512-1658 Sentence denotes Thus, heparin or heparan sulfate is not required for the binding of bFGF to its receptors but increases the binding affinity to a moderate degree.
T10 1659-1757 Sentence denotes Finally, the requirement for heparin in signal transduction through the receptor was investigated.
T10 1659-1757 Sentence denotes Finally, the requirement for heparin in signal transduction through the receptor was investigated.
T11 1758-1891 Sentence denotes Expression of c-fos mRNA was induced by bFGF in 32D cells expressing FGFR-1 to the same extent in the presence or absence of heparin.
T11 1758-1891 Sentence denotes Expression of c-fos mRNA was induced by bFGF in 32D cells expressing FGFR-1 to the same extent in the presence or absence of heparin.