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Id Subject Object Predicate Lexical cue
T1 0-105 Sentence denotes Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells.
T1 0-105 Sentence denotes Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells.
T2 106-278 Sentence denotes The biosynthesis of the prohormone convertase PC2 was studied in Chinese hamster ovary cells stably transfected with PC2 cDNA (CHO/PC2) and in rat insulinoma cells (Rin5f).
T2 106-278 Sentence denotes The biosynthesis of the prohormone convertase PC2 was studied in Chinese hamster ovary cells stably transfected with PC2 cDNA (CHO/PC2) and in rat insulinoma cells (Rin5f).
T3 279-494 Sentence denotes The major form of PC2 synthesized by CHO/PC2 cells was a 75-kDa protein corresponding to proPC2; this protein was retained intracellularly for 2-4 h following synthesis, suggesting prolonged intracellular residence.
T3 279-494 Sentence denotes The major form of PC2 synthesized by CHO/PC2 cells was a 75-kDa protein corresponding to proPC2; this protein was retained intracellularly for 2-4 h following synthesis, suggesting prolonged intracellular residence.
T4 495-651 Sentence denotes In contrast, the major form of PC2 within Rin cells initially exhibited a molecular mass of 72 kDa and was then progressively converted to a 64-kDa species.
T4 495-651 Sentence denotes In contrast, the major form of PC2 within Rin cells initially exhibited a molecular mass of 72 kDa and was then progressively converted to a 64-kDa species.
T5 652-763 Sentence denotes This 64-kDa species, which required 1-2 h to be released, was the major PC2 form detectable in Rin cell medium.
T5 652-763 Sentence denotes This 64-kDa species, which required 1-2 h to be released, was the major PC2 form detectable in Rin cell medium.
T6 764-1000 Sentence denotes Calcium-dependent benzyloxycarbonyl-Arg-Ser-Lys-Arg-aminomethylcoumarin cleaving activity was found in spent Rin cell medium; this activity could be immunoprecipitated with a carboxyl-terminal PC2 antibody, but not with preimmune serum.
T6 764-1000 Sentence denotes Calcium-dependent benzyloxycarbonyl-Arg-Ser-Lys-Arg-aminomethylcoumarin cleaving activity was found in spent Rin cell medium; this activity could be immunoprecipitated with a carboxyl-terminal PC2 antibody, but not with preimmune serum.
T7 1001-1089 Sentence denotes In neither cell line did intracellular PC2 become endoglycosidase H-resistant over time.
T7 1001-1089 Sentence denotes In neither cell line did intracellular PC2 become endoglycosidase H-resistant over time.
T8 1090-1155 Sentence denotes PC2 released from Rin cells was also endoglycosidase H-sensitive.
T8 1090-1155 Sentence denotes PC2 released from Rin cells was also endoglycosidase H-sensitive.
T9 1156-1282 Sentence denotes Microsequencing and endoglycosidase H results indicate that 75-kDa CHO cell PC2 and 72-kDa Rin cell PC2 both represent proPC2.
T9 1156-1282 Sentence denotes Microsequencing and endoglycosidase H results indicate that 75-kDa CHO cell PC2 and 72-kDa Rin cell PC2 both represent proPC2.
T10 1283-1507 Sentence denotes We speculate that (a) PC2 undergoes unusual glycosylation, which may be related to its slow release from cells, and (b) the 64-kDa molecule detectable in spent Rin cell medium represents the enzymatically active form of PC2.
T10 1283-1507 Sentence denotes We speculate that (a) PC2 undergoes unusual glycosylation, which may be related to its slow release from cells, and (b) the 64-kDa molecule detectable in spent Rin cell medium represents the enzymatically active form of PC2.