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PubMed:8227052 JSONTXT 18 Projects

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Id Subject Object Predicate Lexical cue
T1 0-80 Sentence denotes Characterization of the cell adhesion molecule gp24 in Dictyostelium discoideum.
T1 0-80 Sentence denotes Characterization of the cell adhesion molecule gp24 in Dictyostelium discoideum.
T2 81-146 Sentence denotes Mediation of cell-cell adhesion via a Ca(2+)-dependent mechanism.
T2 81-146 Sentence denotes Mediation of cell-cell adhesion via a Ca(2+)-dependent mechanism.
T3 147-332 Sentence denotes Dictyostelium discoideum cells express EDTA-sensitive cell-cell adhesion sites soon after the initiation of development and EDTA-resistant adhesion sites later at the aggregation stage.
T3 147-332 Sentence denotes Dictyostelium discoideum cells express EDTA-sensitive cell-cell adhesion sites soon after the initiation of development and EDTA-resistant adhesion sites later at the aggregation stage.
T4 333-475 Sentence denotes A glycoprotein of M(r) 24,000 (gp24) has been implicated in the mediation of the EDTA-sensitive type of intercellular cohesiveness (Knecht, D.
T4 333-475 Sentence denotes A glycoprotein of M(r) 24,000 (gp24) has been implicated in the mediation of the EDTA-sensitive type of intercellular cohesiveness (Knecht, D.
T5 476-490 Sentence denotes A., Fuller, D.
T5 476-490 Sentence denotes A., Fuller, D.
T6 491-509 Sentence denotes L., and Loomis, W.
T6 491-509 Sentence denotes L., and Loomis, W.
T7 510-524 Sentence denotes F. (1987) Dev.
T7 510-524 Sentence denotes F. (1987) Dev.
T8 525-530 Sentence denotes Biol.
T8 525-530 Sentence denotes Biol.
T9 531-545 Sentence denotes 121, 277-283).
T9 531-545 Sentence denotes 121, 277-283).
T10 546-635 Sentence denotes In this study, a relatively simple procedure was developed to purify gp24 to homogeneity.
T10 546-635 Sentence denotes In this study, a relatively simple procedure was developed to purify gp24 to homogeneity.
T11 636-823 Sentence denotes A highly specific rabbit antiserum was raised against gp24, and the localization of gp24 at the cell surface was shown by quantitative binding of the anti-gp24 antibodies to intact cells.
T11 636-823 Sentence denotes A highly specific rabbit antiserum was raised against gp24, and the localization of gp24 at the cell surface was shown by quantitative binding of the anti-gp24 antibodies to intact cells.
T12 824-935 Sentence denotes To demonstrate the cell binding activity of gp24, the binding of solubilized gp24 to intact cells was examined.
T12 824-935 Sentence denotes To demonstrate the cell binding activity of gp24, the binding of solubilized gp24 to intact cells was examined.
T13 936-1072 Sentence denotes 125I-Labeled gp24 bound to cells in a dose-dependent and saturable manner, and the binding was displaced specifically by unlabeled gp24.
T13 936-1072 Sentence denotes 125I-Labeled gp24 bound to cells in a dose-dependent and saturable manner, and the binding was displaced specifically by unlabeled gp24.
T14 1073-1199 Sentence denotes Purified gp24 was capable of inhibiting the reassociation of dispersed cells previously undergoing EDTA-sensitive aggregation.
T14 1073-1199 Sentence denotes Purified gp24 was capable of inhibiting the reassociation of dispersed cells previously undergoing EDTA-sensitive aggregation.
T15 1200-1314 Sentence denotes Moreover, precoating cells with anti-gp24 IgG and Fab fragments blocked the binding of 125I-labeled gp24 to cells.
T15 1200-1314 Sentence denotes Moreover, precoating cells with anti-gp24 IgG and Fab fragments blocked the binding of 125I-labeled gp24 to cells.
T16 1315-1481 Sentence denotes Collectively, these in vitro assays provide direct evidence that gp24 is a cell adhesion molecule that most likely functions through a homophilic mode of interaction.
T16 1315-1481 Sentence denotes Collectively, these in vitro assays provide direct evidence that gp24 is a cell adhesion molecule that most likely functions through a homophilic mode of interaction.
T17 1482-1596 Sentence denotes The binding of gp24 to cells was sensitive to EGTA, suggesting that the activity of gp24 may involve calcium ions.
T17 1482-1596 Sentence denotes The binding of gp24 to cells was sensitive to EGTA, suggesting that the activity of gp24 may involve calcium ions.
T18 1597-1688 Sentence denotes Binding studies showed that 45Ca2+ could bind to gp24 blotted onto nitrocellulose membrane.
T18 1597-1688 Sentence denotes Binding studies showed that 45Ca2+ could bind to gp24 blotted onto nitrocellulose membrane.
T19 1689-1796 Sentence denotes In addition, preincubation of the native protein with calcium ions resulted in a shift in its gel mobility.
T19 1689-1796 Sentence denotes In addition, preincubation of the native protein with calcium ions resulted in a shift in its gel mobility.
T20 1797-2003 Sentence denotes It is therefore likely that gp24 mediates cell-cell interactions via a Ca(2+)-dependent mechanism, rendering gp24 the first cell adhesion molecule in D. discoideum to utilize a Ca(2+)-based adhesion system.
T20 1797-2003 Sentence denotes It is therefore likely that gp24 mediates cell-cell interactions via a Ca(2+)-dependent mechanism, rendering gp24 the first cell adhesion molecule in D. discoideum to utilize a Ca(2+)-based adhesion system.