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PubMed:819433 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-60 Sentence denotes Ecto-5'-nucleotidase of intact cultured C6 rat glioma cells.
T1 0-60 Sentence denotes Ecto-5'-nucleotidase of intact cultured C6 rat glioma cells.
T2 61-170 Sentence denotes The characteristics of 5'-nucleotidase in a clonal line (C6) of rat glioma cells has been examined in detail.
T2 61-170 Sentence denotes The characteristics of 5'-nucleotidase in a clonal line (C6) of rat glioma cells has been examined in detail.
T3 171-367 Sentence denotes The cells liberated 6.80 +/- 0.33 mumol of inorganic phosphate/mg of cell protein/hour, producing nearly equimolar amounts of adenosine and inorganic phosphate from AMP in the extracellular fluid.
T3 171-367 Sentence denotes The cells liberated 6.80 +/- 0.33 mumol of inorganic phosphate/mg of cell protein/hour, producing nearly equimolar amounts of adenosine and inorganic phosphate from AMP in the extracellular fluid.
T4 368-429 Sentence denotes No 5'-nucleotidase was released by the cells into the medium.
T4 368-429 Sentence denotes No 5'-nucleotidase was released by the cells into the medium.
T5 430-701 Sentence denotes Most of the 5'-nucleotidase activity was found to be located in the outer surface of the plasma membrane of C6 cells and rapidly accessible to exogenous AMP, by experiments based upon differential labeling of extracellular and intracellular compartments with 32P and 33P.
T5 430-701 Sentence denotes Most of the 5'-nucleotidase activity was found to be located in the outer surface of the plasma membrane of C6 cells and rapidly accessible to exogenous AMP, by experiments based upon differential labeling of extracellular and intracellular compartments with 32P and 33P.
T6 702-764 Sentence denotes The ecto-enzyme was active in the absence of divalent cations.
T6 702-764 Sentence denotes The ecto-enzyme was active in the absence of divalent cations.
T7 765-813 Sentence denotes However, Mn2+ or Co2+ were somewhat stimulatory.
T7 765-813 Sentence denotes However, Mn2+ or Co2+ were somewhat stimulatory.
T8 814-853 Sentence denotes Zn2+ suppressed activity very markedly.
T8 814-853 Sentence denotes Zn2+ suppressed activity very markedly.
T9 854-973 Sentence denotes The relationship of enzymatic reaction velocity to pH was complex, with an optimum at pH 7.4 for all substrates tested.
T9 854-973 Sentence denotes The relationship of enzymatic reaction velocity to pH was complex, with an optimum at pH 7.4 for all substrates tested.
T10 974-1036 Sentence denotes The ecto-5'-nucleotidase readily hydrolyzed 5'-AMP and 5'-UMP.
T10 974-1036 Sentence denotes The ecto-5'-nucleotidase readily hydrolyzed 5'-AMP and 5'-UMP.
T11 1037-1190 Sentence denotes Other 5'-nucleoside monophosphates, including 5'-deoxy-AMP, were also hydrolyzed, but more slowly; 2'- or 3'-nucleoside monophosphates were not attacked.
T11 1037-1190 Sentence denotes Other 5'-nucleoside monophosphates, including 5'-deoxy-AMP, were also hydrolyzed, but more slowly; 2'- or 3'-nucleoside monophosphates were not attacked.
T12 1191-1268 Sentence denotes The ecto-5'-nucleotidase in the intact cell obeyed Michaelis-Menten kinetics.
T12 1191-1268 Sentence denotes The ecto-5'-nucleotidase in the intact cell obeyed Michaelis-Menten kinetics.
T13 1269-1387 Sentence denotes Apparent Km for AMP was 0.22 mM; apparent Km values for other substrates were similar and ranged from 0.16 to 0.18 mM.
T13 1269-1387 Sentence denotes Apparent Km for AMP was 0.22 mM; apparent Km values for other substrates were similar and ranged from 0.16 to 0.18 mM.
T14 1388-1538 Sentence denotes ADP exerted a very powerful inhibitory effect, behaving as a competitive inhibitor, and 5'-UMP behaved as a strictly competitive substrate for 5'-AMP.
T14 1388-1538 Sentence denotes ADP exerted a very powerful inhibitory effect, behaving as a competitive inhibitor, and 5'-UMP behaved as a strictly competitive substrate for 5'-AMP.
T15 1539-1567 Sentence denotes ATP and ITP were inhibitory.
T15 1539-1567 Sentence denotes ATP and ITP were inhibitory.
T16 1568-1612 Sentence denotes Of these, ITP served to increase Km for AMP.
T16 1568-1612 Sentence denotes Of these, ITP served to increase Km for AMP.
T17 1613-1661 Sentence denotes ATP did likewise, but also greatly lowered Vmax.
T17 1613-1661 Sentence denotes ATP did likewise, but also greatly lowered Vmax.
T18 1662-1925 Sentence denotes These findings indicate that the intact cell is capable of rapid hydrolysis of exogenous 5'-AMP, to produce adenosine at the cell surface at a rate which responds directly to extracellular AMP concentration but which can be suppressed by extracellular ADP or ATP.
T18 1662-1925 Sentence denotes These findings indicate that the intact cell is capable of rapid hydrolysis of exogenous 5'-AMP, to produce adenosine at the cell surface at a rate which responds directly to extracellular AMP concentration but which can be suppressed by extracellular ADP or ATP.