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PubMed:8132603 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-91 Sentence denotes Identification of the site of phosphorylation on the osmosensor, EnvZ, of Escherichia coli.
T1 0-91 Sentence denotes Identification of the site of phosphorylation on the osmosensor, EnvZ, of Escherichia coli.
T2 92-244 Sentence denotes EnvZ is a membrane-bound histidine kinase that functions as an osmotic sensor capable of phosphorylating the regulator protein OmpR in Escherichia coli.
T2 92-244 Sentence denotes EnvZ is a membrane-bound histidine kinase that functions as an osmotic sensor capable of phosphorylating the regulator protein OmpR in Escherichia coli.
T3 245-415 Sentence denotes To characterize the site of phosphorylation biochemically, we overexpressed a 36-kDa truncated EnvZ protein (Glu-106 to Gly-450) that formed inclusion bodies in the cell.
T3 245-415 Sentence denotes To characterize the site of phosphorylation biochemically, we overexpressed a 36-kDa truncated EnvZ protein (Glu-106 to Gly-450) that formed inclusion bodies in the cell.
T4 416-551 Sentence denotes After solubilization, the inclusion body form of EnvZ was cleaved into two major fragments with molecular weights of 25,000 and 10,000.
T4 416-551 Sentence denotes After solubilization, the inclusion body form of EnvZ was cleaved into two major fragments with molecular weights of 25,000 and 10,000.
T5 552-623 Sentence denotes The 25-kDa fragment, EnvZc, was purified and found to exist as a dimer.
T5 552-623 Sentence denotes The 25-kDa fragment, EnvZc, was purified and found to exist as a dimer.
T6 624-775 Sentence denotes N-terminal sequence analysis established that cleavage had occurred at Arg-214, indicating that EnvZc contained most of the cytoplasmic domain of EnvZ.
T6 624-775 Sentence denotes N-terminal sequence analysis established that cleavage had occurred at Arg-214, indicating that EnvZc contained most of the cytoplasmic domain of EnvZ.
T7 776-979 Sentence denotes After labeling EnvZc with [gamma-32P]ATP, the protein was proteolytically digested, and the resulting peptides were separated by reverse phase chromatography using high performance liquid chromatography.
T7 776-979 Sentence denotes After labeling EnvZc with [gamma-32P]ATP, the protein was proteolytically digested, and the resulting peptides were separated by reverse phase chromatography using high performance liquid chromatography.
T8 980-1101 Sentence denotes One major radioactive peptide containing greater than 90% of the recovered peptide-associated radioactivity was isolated.
T8 980-1101 Sentence denotes One major radioactive peptide containing greater than 90% of the recovered peptide-associated radioactivity was isolated.
T9 1102-1231 Sentence denotes Amino acid analysis of this purified peptide indicated that the composition was consistent with a peptide that contained His-243.
T9 1102-1231 Sentence denotes Amino acid analysis of this purified peptide indicated that the composition was consistent with a peptide that contained His-243.
T10 1232-1324 Sentence denotes The amino acid sequence of this peptide was determined to be MAGVSHDLRTP (residues 238-248).
T10 1232-1324 Sentence denotes The amino acid sequence of this peptide was determined to be MAGVSHDLRTP (residues 238-248).
T11 1325-1586 Sentence denotes These results indicate that His-243 is the major site of phosphorylation on EnvZ and represents the first biochemical characterization of the site of phosphorylation of a membrane histidine kinase of the two-component regulatory family of molecules in bacteria.
T11 1325-1586 Sentence denotes These results indicate that His-243 is the major site of phosphorylation on EnvZ and represents the first biochemical characterization of the site of phosphorylation of a membrane histidine kinase of the two-component regulatory family of molecules in bacteria.