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PubMed:807579 JSONTXT 19 Projects

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Id Subject Object Predicate Lexical cue
T1 0-24 Sentence denotes Biosynthesis of heparin.
T1 0-24 Sentence denotes Biosynthesis of heparin.
T2 25-69 Sentence denotes Studies on the microsomal sulfation process.
T2 25-69 Sentence denotes Studies on the microsomal sulfation process.
T3 70-262 Sentence denotes Nonsulfated, partially N-deacetylated, 14C-labeled polysaccharide was formed by incubation of a mouse mastocytoma microsomal fraction with UDP-[14C]glucuronic acid and UDP-N-acetylglucosamine.
T3 70-262 Sentence denotes Nonsulfated, partially N-deacetylated, 14C-labeled polysaccharide was formed by incubation of a mouse mastocytoma microsomal fraction with UDP-[14C]glucuronic acid and UDP-N-acetylglucosamine.
T4 263-463 Sentence denotes After 60 min at 37 degrees the incorporation of radioactivity was interrupted, and sulfation was initiated, by the addition of unlabeled UDP-glucuronic acid and 3-phosphoadenylylsulfate, respectively.
T4 263-463 Sentence denotes After 60 min at 37 degrees the incorporation of radioactivity was interrupted, and sulfation was initiated, by the addition of unlabeled UDP-glucuronic acid and 3-phosphoadenylylsulfate, respectively.
T5 464-606 Sentence denotes After varying periods of incubation with the nucleotide sulfate, polysaccharide was isolated and analyzed by chromatography on DEAE-cellulose.
T5 464-606 Sentence denotes After varying periods of incubation with the nucleotide sulfate, polysaccharide was isolated and analyzed by chromatography on DEAE-cellulose.
T6 607-862 Sentence denotes During incubation with 3-phosphoadenylylsulfate, sulfated 14C-polysaccharides were formed, while the nonsulfated 14C-polysaccharide was eliminated, each type of component retaining the same elution position throughout the entire sulfation period (60 min).
T6 607-862 Sentence denotes During incubation with 3-phosphoadenylylsulfate, sulfated 14C-polysaccharides were formed, while the nonsulfated 14C-polysaccharide was eliminated, each type of component retaining the same elution position throughout the entire sulfation period (60 min).
T7 863-917 Sentence denotes No accumulation of low sulfated material was observed.
T7 863-917 Sentence denotes No accumulation of low sulfated material was observed.
T8 918-986 Sentence denotes Fully sulfated heparin was detected after only 0.5 min of sulfation.
T8 918-986 Sentence denotes Fully sulfated heparin was detected after only 0.5 min of sulfation.
T9 987-1124 Sentence denotes These results suggest that the sulfation of heparin is a rapid process, involving a limited number of polysaccharide molecules at a time.
T9 987-1124 Sentence denotes These results suggest that the sulfation of heparin is a rapid process, involving a limited number of polysaccharide molecules at a time.
T10 1125-1344 Sentence denotes Besides heparin-like products, having both N- and O-sulfate groups, and [14C]iduronic acid as predominant uronic acid constituent, an additional sulfated component was detected, having N-sulfate but no O-sulfate groups.
T10 1125-1344 Sentence denotes Besides heparin-like products, having both N- and O-sulfate groups, and [14C]iduronic acid as predominant uronic acid constituent, an additional sulfated component was detected, having N-sulfate but no O-sulfate groups.
T11 1345-1438 Sentence denotes This novel polysaccharide contained [14C]glucuronic acid but little or no [14C]iduronic acid.
T11 1345-1438 Sentence denotes This novel polysaccharide contained [14C]glucuronic acid but little or no [14C]iduronic acid.
T12 1439-1675 Sentence denotes Previous studies have shown that L-iduronic acid residues in heparin are formed by C-5 inversion of D-glucuronic acid units, previously incorporated into the polymer; the inversion reaction requires concomitant sulfation of the polymer.
T12 1439-1675 Sentence denotes Previous studies have shown that L-iduronic acid residues in heparin are formed by C-5 inversion of D-glucuronic acid units, previously incorporated into the polymer; the inversion reaction requires concomitant sulfation of the polymer.
T13 1676-1800 Sentence denotes The present results demonstrate that the formation of iduronic acid residues is related to the sulfation of hydroxyl groups.
T13 1676-1800 Sentence denotes The present results demonstrate that the formation of iduronic acid residues is related to the sulfation of hydroxyl groups.
T14 1801-2016 Sentence denotes Pulse-chase experiments with 3-phosphoadenylyl[35S]sulfate indicated that the N-sulfated polymer, lacking O-sulfate groups, could be converted into heparin-like products by further sulfation of hydroxyl groups only.
T14 1801-2016 Sentence denotes Pulse-chase experiments with 3-phosphoadenylyl[35S]sulfate indicated that the N-sulfated polymer, lacking O-sulfate groups, could be converted into heparin-like products by further sulfation of hydroxyl groups only.
T15 2017-2170 Sentence denotes The biosynthesis of heparin may thus involve a pathway, in which the formation of N-sulfated intermediates precedes the introduction of O-sulfate groups.
T15 2017-2170 Sentence denotes The biosynthesis of heparin may thus involve a pathway, in which the formation of N-sulfated intermediates precedes the introduction of O-sulfate groups.