| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-99 |
Sentence |
denotes |
Erythropoietin-dependent induction of hemoglobin synthesis in a cytokine-dependent cell line M-TAT. |
| T1 |
0-99 |
Sentence |
denotes |
Erythropoietin-dependent induction of hemoglobin synthesis in a cytokine-dependent cell line M-TAT. |
| T2 |
100-224 |
Sentence |
denotes |
M-TAT is a cytokine-dependent cell line with the potential to differentiate along the erythroid and megakaryocytic lineages. |
| T2 |
100-224 |
Sentence |
denotes |
M-TAT is a cytokine-dependent cell line with the potential to differentiate along the erythroid and megakaryocytic lineages. |
| T3 |
225-407 |
Sentence |
denotes |
We cultured M-TAT cells long term (> 1 year) in the continuous presence of erythropoietin (EPO), granulocyte-macrophage colony-stimulating factor (GM-CSF), or stem cell factor (SCF). |
| T3 |
225-407 |
Sentence |
denotes |
We cultured M-TAT cells long term (> 1 year) in the continuous presence of erythropoietin (EPO), granulocyte-macrophage colony-stimulating factor (GM-CSF), or stem cell factor (SCF). |
| T4 |
408-511 |
Sentence |
denotes |
These long term cultures are referred to as M-TAT/EPO, M-TAT/GM-CSF, and M-TAT/SCF cells, respectively. |
| T4 |
408-511 |
Sentence |
denotes |
These long term cultures are referred to as M-TAT/EPO, M-TAT/GM-CSF, and M-TAT/SCF cells, respectively. |
| T5 |
512-683 |
Sentence |
denotes |
Hemoglobin concentration and gamma-globin and erythroid delta-aminolevulinate synthase mRNA levels were significantly higher in M-TAT/EPO cells than in M-TAT/GM-CSF cells. |
| T5 |
512-683 |
Sentence |
denotes |
Hemoglobin concentration and gamma-globin and erythroid delta-aminolevulinate synthase mRNA levels were significantly higher in M-TAT/EPO cells than in M-TAT/GM-CSF cells. |
| T6 |
684-862 |
Sentence |
denotes |
When the supplemented cytokine was switched from GM-CSF to EPO, hemoglobin synthesis in M-TAT/GM-CSF cells increased rapidly (within 5 h), and the level of GATA-1 mRNA increased. |
| T6 |
684-862 |
Sentence |
denotes |
When the supplemented cytokine was switched from GM-CSF to EPO, hemoglobin synthesis in M-TAT/GM-CSF cells increased rapidly (within 5 h), and the level of GATA-1 mRNA increased. |
| T7 |
863-1079 |
Sentence |
denotes |
In contrast, the addition of GM-CSF to the M-TAT/EPO cell culture decreased the amount of hemoglobin, even in the presence of EPO, indicating that the EPO signal for erythroid differentiation is suppressed by GM-CSF. |
| T7 |
863-1079 |
Sentence |
denotes |
In contrast, the addition of GM-CSF to the M-TAT/EPO cell culture decreased the amount of hemoglobin, even in the presence of EPO, indicating that the EPO signal for erythroid differentiation is suppressed by GM-CSF. |
| T8 |
1080-1167 |
Sentence |
denotes |
Thus, erythroid development of M-TAT cells is promoted by EPO and suppressed by GM-CSF. |
| T8 |
1080-1167 |
Sentence |
denotes |
Thus, erythroid development of M-TAT cells is promoted by EPO and suppressed by GM-CSF. |
| T9 |
1168-1324 |
Sentence |
denotes |
These results support the hypothesis that EPO actively influences the programming of gene expression required for erythroid progenitor cell differentiation. |
| T9 |
1168-1324 |
Sentence |
denotes |
These results support the hypothesis that EPO actively influences the programming of gene expression required for erythroid progenitor cell differentiation. |