| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-102 |
Sentence |
denotes |
Transcriptional regulation of the vacuolar H(+)-ATPase B2 subunit gene in differentiating THP-1 cells. |
| T1 |
0-102 |
Sentence |
denotes |
Transcriptional regulation of the vacuolar H(+)-ATPase B2 subunit gene in differentiating THP-1 cells. |
| T2 |
103-240 |
Sentence |
denotes |
Monocyte-macrophage differentiation was used as a model system for studying gene regulation of the human vacuolar H(+)-ATPase (V-ATPase). |
| T2 |
103-240 |
Sentence |
denotes |
Monocyte-macrophage differentiation was used as a model system for studying gene regulation of the human vacuolar H(+)-ATPase (V-ATPase). |
| T3 |
241-487 |
Sentence |
denotes |
We examined mRNA levels of various V-ATPase subunits during differentiation of both native monocytes and the cell line THP-1, and found that transcriptional and post-transcriptional mechanisms could account for increases in cell V-ATPase content. |
| T3 |
241-487 |
Sentence |
denotes |
We examined mRNA levels of various V-ATPase subunits during differentiation of both native monocytes and the cell line THP-1, and found that transcriptional and post-transcriptional mechanisms could account for increases in cell V-ATPase content. |
| T4 |
488-641 |
Sentence |
denotes |
From nuclear runoff experiments, we found that one subunit in particular, the B2 isoform (Mr = 56,000), was amplified primarily by transcriptional means. |
| T4 |
488-641 |
Sentence |
denotes |
From nuclear runoff experiments, we found that one subunit in particular, the B2 isoform (Mr = 56,000), was amplified primarily by transcriptional means. |
| T5 |
642-715 |
Sentence |
denotes |
We have begun to examine the structure of the B2 subunit promoter region. |
| T5 |
642-715 |
Sentence |
denotes |
We have begun to examine the structure of the B2 subunit promoter region. |
| T6 |
716-849 |
Sentence |
denotes |
Isolation and sequencing of the first exon and 5'-flanking region of this gene reveal a TATA-less promoter with a high G + C content. |
| T6 |
716-849 |
Sentence |
denotes |
Isolation and sequencing of the first exon and 5'-flanking region of this gene reveal a TATA-less promoter with a high G + C content. |
| T7 |
850-955 |
Sentence |
denotes |
Primer extension and ribonuclease protection analyses indicate a single major transcriptional start site. |
| T7 |
850-955 |
Sentence |
denotes |
Primer extension and ribonuclease protection analyses indicate a single major transcriptional start site. |
| T8 |
956-1115 |
Sentence |
denotes |
We transfected promoter-luciferase reporter plasmids into THP-1 cells to define sequences that mediate transcriptional control during monocyte differentiation. |
| T8 |
956-1115 |
Sentence |
denotes |
We transfected promoter-luciferase reporter plasmids into THP-1 cells to define sequences that mediate transcriptional control during monocyte differentiation. |
| T9 |
1116-1263 |
Sentence |
denotes |
We found that sequences downstream from the transcriptional start site were sufficient to confer increased expression during THP-1 differentiation. |
| T9 |
1116-1263 |
Sentence |
denotes |
We found that sequences downstream from the transcriptional start site were sufficient to confer increased expression during THP-1 differentiation. |
| T10 |
1264-1419 |
Sentence |
denotes |
DNase I footprinting and sequence analysis revealed the existence of multiple AP2 and Sp1 binding sites in the 5'-untranslated and proximal coding regions. |
| T10 |
1264-1419 |
Sentence |
denotes |
DNase I footprinting and sequence analysis revealed the existence of multiple AP2 and Sp1 binding sites in the 5'-untranslated and proximal coding regions. |