| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-140 |
Sentence |
denotes |
Direct demonstration of NFATp dephosphorylation and nuclear localization in activated HT-2 cells using a specific NFATp polyclonal antibody. |
| T1 |
0-140 |
Sentence |
denotes |
Direct demonstration of NFATp dephosphorylation and nuclear localization in activated HT-2 cells using a specific NFATp polyclonal antibody. |
| T2 |
141-311 |
Sentence |
denotes |
Nuclear factor of activated T cells (NFAT) regulates transcription of a number of cytokine genes, and NFAT DNA binding activity is stimulated following T cell activation. |
| T2 |
141-311 |
Sentence |
denotes |
Nuclear factor of activated T cells (NFAT) regulates transcription of a number of cytokine genes, and NFAT DNA binding activity is stimulated following T cell activation. |
| T3 |
312-426 |
Sentence |
denotes |
Several lines of evidence have suggested that NFAT is a substrate for calcineurin, a serine/threonine phosphatase. |
| T3 |
312-426 |
Sentence |
denotes |
Several lines of evidence have suggested that NFAT is a substrate for calcineurin, a serine/threonine phosphatase. |
| T4 |
427-609 |
Sentence |
denotes |
Using a polyclonal antibody to murine NFATp, Western blot analysis of various mouse tissues demonstrated that the 110-130-kDa NFATp protein was highly expressed in thymus and spleen. |
| T4 |
427-609 |
Sentence |
denotes |
Using a polyclonal antibody to murine NFATp, Western blot analysis of various mouse tissues demonstrated that the 110-130-kDa NFATp protein was highly expressed in thymus and spleen. |
| T5 |
610-801 |
Sentence |
denotes |
Treatment of immunoprecipitated NFATp from untreated HT-2 cells with calcineurin resulted in the dephosphorylation of NFATp, demonstrating that NFATp is an in vitro substrate for calcineurin. |
| T5 |
610-801 |
Sentence |
denotes |
Treatment of immunoprecipitated NFATp from untreated HT-2 cells with calcineurin resulted in the dephosphorylation of NFATp, demonstrating that NFATp is an in vitro substrate for calcineurin. |
| T6 |
802-947 |
Sentence |
denotes |
NFATp immunoprecipitated from 32P-labeled HT-2 cells migrated as an approximately 120-kDa protein that was localized to the cytosol of the cells. |
| T6 |
802-947 |
Sentence |
denotes |
NFATp immunoprecipitated from 32P-labeled HT-2 cells migrated as an approximately 120-kDa protein that was localized to the cytosol of the cells. |
| T7 |
948-1101 |
Sentence |
denotes |
Treatment of the cells with ionomycin resulted in a decrease in the molecular weight of NFATp and a loss of 32P, consistent with NFATp dephosphorylation. |
| T7 |
948-1101 |
Sentence |
denotes |
Treatment of the cells with ionomycin resulted in a decrease in the molecular weight of NFATp and a loss of 32P, consistent with NFATp dephosphorylation. |
| T8 |
1102-1204 |
Sentence |
denotes |
The dephosphorylation of NFATp was accompanied by localization of the protein to the nuclear fraction. |
| T8 |
1102-1204 |
Sentence |
denotes |
The dephosphorylation of NFATp was accompanied by localization of the protein to the nuclear fraction. |
| T9 |
1205-1384 |
Sentence |
denotes |
Both of these events were blocked by preincubation of the cells with FK506, a calcineurin inhibitor, consistent with the hypothesis that NFATp is a calcineurin substrate in cells. |
| T9 |
1205-1384 |
Sentence |
denotes |
Both of these events were blocked by preincubation of the cells with FK506, a calcineurin inhibitor, consistent with the hypothesis that NFATp is a calcineurin substrate in cells. |