| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-160 |
Sentence |
denotes |
Identification of an I kappa B alpha-associated protein kinase in a human monocytic cell line and determination of its phosphorylation sites on I kappa B alpha. |
| T1 |
0-160 |
Sentence |
denotes |
Identification of an I kappa B alpha-associated protein kinase in a human monocytic cell line and determination of its phosphorylation sites on I kappa B alpha. |
| T2 |
161-279 |
Sentence |
denotes |
Nuclear factor kappa B (NF-kappa B) is stored in the cytoplasm as an inactive form through interaction with I kappa B. |
| T2 |
161-279 |
Sentence |
denotes |
Nuclear factor kappa B (NF-kappa B) is stored in the cytoplasm as an inactive form through interaction with I kappa B. |
| T3 |
280-419 |
Sentence |
denotes |
Stimulation of cells leads to a rapid phosphorylation of I kappa B alpha, which is presumed to be important for the subsequent degradation. |
| T3 |
280-419 |
Sentence |
denotes |
Stimulation of cells leads to a rapid phosphorylation of I kappa B alpha, which is presumed to be important for the subsequent degradation. |
| T4 |
420-615 |
Sentence |
denotes |
We have recently reported the establishment of a lipopolysaccharide (LPS)-dependent cell-free activation system of NF-kappa B in association with the induction of I kappa B alpha phosphorylation. |
| T4 |
420-615 |
Sentence |
denotes |
We have recently reported the establishment of a lipopolysaccharide (LPS)-dependent cell-free activation system of NF-kappa B in association with the induction of I kappa B alpha phosphorylation. |
| T5 |
616-793 |
Sentence |
denotes |
In this study, we have identified a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically binds and phosphorylates I kappa B alpha. |
| T5 |
616-793 |
Sentence |
denotes |
In this study, we have identified a kinase in cell extracts from the LPS-stimulated human monocytic cell line, THP-1, that specifically binds and phosphorylates I kappa B alpha. |
| T6 |
794-888 |
Sentence |
denotes |
LPS stimulation transiently enhanced the I kappa B alpha-bound kinase activity in THP-1 cells. |
| T6 |
794-888 |
Sentence |
denotes |
LPS stimulation transiently enhanced the I kappa B alpha-bound kinase activity in THP-1 cells. |
| T7 |
889-1118 |
Sentence |
denotes |
Mutational analyses of I kappa B alpha and competition experiments with the synthetic peptides identified major phosphorylation sites by the bound kinase as Ser and Thr residues in the C-terminal acidic domain of I kappa B alpha. |
| T7 |
889-1118 |
Sentence |
denotes |
Mutational analyses of I kappa B alpha and competition experiments with the synthetic peptides identified major phosphorylation sites by the bound kinase as Ser and Thr residues in the C-terminal acidic domain of I kappa B alpha. |
| T8 |
1119-1374 |
Sentence |
denotes |
Moreover, we show that the peptide, corresponding to the C-terminal acidic domain of I kappa B alpha, blocked the LPS-induced NF-kappa B activation as well as inducible phosphorylation of endogenous I kappa B alpha in a cell-free system using THP-1 cells. |
| T8 |
1119-1374 |
Sentence |
denotes |
Moreover, we show that the peptide, corresponding to the C-terminal acidic domain of I kappa B alpha, blocked the LPS-induced NF-kappa B activation as well as inducible phosphorylation of endogenous I kappa B alpha in a cell-free system using THP-1 cells. |
| T9 |
1375-1611 |
Sentence |
denotes |
These results suggested that the bound kinase is involved in the signaling pathway of LPS by inducing the phosphorylation of the C-terminal region of I kappa B alpha and subsequent dissociation of the NF-kappa B.I kappa B alpha complex. |
| T9 |
1375-1611 |
Sentence |
denotes |
These results suggested that the bound kinase is involved in the signaling pathway of LPS by inducing the phosphorylation of the C-terminal region of I kappa B alpha and subsequent dissociation of the NF-kappa B.I kappa B alpha complex. |
