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PubMed:7496140 JSONTXT 58 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-93 Sentence denotes Flow cytofluorimetric analysis of young and senescent human erythrocytes probed with lectins.
T1 0-93 Sentence denotes Flow cytofluorimetric analysis of young and senescent human erythrocytes probed with lectins.
TextSentencer_T2 94-145 Sentence denotes Evidence that sialic acids control their life span.
T2 94-145 Sentence denotes Evidence that sialic acids control their life span.
TextSentencer_T3 146-497 Sentence denotes Comparing the properties of 'young' and senescent ('aged') O+ erythrocytes isolated by applying ultracentrifugation in a self-forming Percoll gradient, we demonstrate that the sialic acids of membrane glycoconjugates control the life span of erythrocytes and that the desialylation of glycans is responsible for the clearance of the aged erythrocytes.
T3 146-497 Sentence denotes Comparing the properties of 'young' and senescent ('aged') O+ erythrocytes isolated by applying ultracentrifugation in a self-forming Percoll gradient, we demonstrate that the sialic acids of membrane glycoconjugates control the life span of erythrocytes and that the desialylation of glycans is responsible for the clearance of the aged erythrocytes.
TextSentencer_T4 498-586 Sentence denotes This capture is mediated by a beta-galactolectin present in the membrane of macrophages.
T4 498-586 Sentence denotes This capture is mediated by a beta-galactolectin present in the membrane of macrophages.
TextSentencer_T5 587-858 Sentence denotes The evidence supporting these conclusions is as follows: (1) Analysis by flow cytofluorimetry of the binding of fluorescein isothiocyanate labelled lectins specific for sialic acids shows that the aged erythrocytes bind less WGA, LPA, SNA and MAA than young erythrocytes.
T5 587-858 Sentence denotes The evidence supporting these conclusions is as follows: (1) Analysis by flow cytofluorimetry of the binding of fluorescein isothiocyanate labelled lectins specific for sialic acids shows that the aged erythrocytes bind less WGA, LPA, SNA and MAA than young erythrocytes.
TextSentencer_T6 859-902 Sentence denotes The binding of DSA and LCA is not modified.
T6 859-902 Sentence denotes The binding of DSA and LCA is not modified.
TextSentencer_T7 903-1015 Sentence denotes On the contrary, the number of binding sites of UEA-I specific for O antigen and of AAA decreases significantly.
T7 903-1015 Sentence denotes On the contrary, the number of binding sites of UEA-I specific for O antigen and of AAA decreases significantly.
TextSentencer_T8 1016-1423 Sentence denotes PNA and GNA do not bind to erythrocytes. (2) RCA120 as well as Erythrina cristagalli and Erythrina corallodendron lectins specific for terminal beta-galactose residues lead to unexpected and unexplained results with a decrease in the number of lectin binding sites associated with increasing desialylation. (3) The glycoconjugates from the old erythrocytes incorporate more sialic acid than the young cells.
T8 1016-1423 Sentence denotes PNA and GNA do not bind to erythrocytes. (2) RCA120 as well as Erythrina cristagalli and Erythrina corallodendron lectins specific for terminal beta-galactose residues lead to unexpected and unexplained results with a decrease in the number of lectin binding sites associated with increasing desialylation. (3) The glycoconjugates from the old erythrocytes incorporate more sialic acid than the young cells.
TextSentencer_T9 1424-1780 Sentence denotes This observation results from the determination of the rate of transfer by alpha-2,6-sialyltransferase of fluorescent or radioactive N-acetylneuraminic acid, using as donors CMP-9-fluoresceinyl-NeuAc and CMP-[14C]-NeuAc, respectively. (4) Microscopy shows that the old erythrocytes are captured preferentially by the macrophages relative to the young ones.
T9 1424-1780 Sentence denotes This observation results from the determination of the rate of transfer by alpha-2,6-sialyltransferase of fluorescent or radioactive N-acetylneuraminic acid, using as donors CMP-9-fluoresceinyl-NeuAc and CMP-[14C]-NeuAc, respectively. (4) Microscopy shows that the old erythrocytes are captured preferentially by the macrophages relative to the young ones.
TextSentencer_T10 1781-2211 Sentence denotes Fixation of erythrocytes by the macrophage membrane is inhibited by lactose, thus demonstrating the involvement of a terminal beta-galactose specific macrophage lectin. (5) Comparative study of the binding of WGA, LPA, SNA and MAA to the aged erythrocytes and to the in vitro enzymatically desialylated erythrocytes shows that the desialylation rate of aged cells is low but sufficient to lead to their capture by the macrophages.
T10 1781-2211 Sentence denotes Fixation of erythrocytes by the macrophage membrane is inhibited by lactose, thus demonstrating the involvement of a terminal beta-galactose specific macrophage lectin. (5) Comparative study of the binding of WGA, LPA, SNA and MAA to the aged erythrocytes and to the in vitro enzymatically desialylated erythrocytes shows that the desialylation rate of aged cells is low but sufficient to lead to their capture by the macrophages.