| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-85 |
Sentence |
denotes |
Topography of sialoglycoproteins and sialyltransferases in mouse and rat liver Golgi. |
| T1 |
0-85 |
Sentence |
denotes |
Topography of sialoglycoproteins and sialyltransferases in mouse and rat liver Golgi. |
| T2 |
86-231 |
Sentence |
denotes |
We have studied the membrane topography of N-acetylneuraminic acid (NeuAc) in sialoglycoproteins of mouse and rat liver Golgi apparatus vesicles. |
| T2 |
86-231 |
Sentence |
denotes |
We have studied the membrane topography of N-acetylneuraminic acid (NeuAc) in sialoglycoproteins of mouse and rat liver Golgi apparatus vesicles. |
| T3 |
232-394 |
Sentence |
denotes |
Purified Golgi vesicles or Golgi vesicles present in microsomes were able to incorporate radiolabeled NeuAc from CMP-NeuAc into endogenous glycoproteins in vitro. |
| T3 |
232-394 |
Sentence |
denotes |
Purified Golgi vesicles or Golgi vesicles present in microsomes were able to incorporate radiolabeled NeuAc from CMP-NeuAc into endogenous glycoproteins in vitro. |
| T4 |
395-575 |
Sentence |
denotes |
The labeled glycoproteins showed a similar profile on sodium dodecyl sulfate gel electrophoresis to those isolated from Golgi fractions of animals injected with radiolabeled NeuAc. |
| T4 |
395-575 |
Sentence |
denotes |
The labeled glycoproteins showed a similar profile on sodium dodecyl sulfate gel electrophoresis to those isolated from Golgi fractions of animals injected with radiolabeled NeuAc. |
| T5 |
576-755 |
Sentence |
denotes |
Using this labeling procedure in vitro, we found that only 5 to 20% of the incorporated NeuAc could be removed from intact vesicles upon treatment with neuraminidase or proteases. |
| T5 |
576-755 |
Sentence |
denotes |
Using this labeling procedure in vitro, we found that only 5 to 20% of the incorporated NeuAc could be removed from intact vesicles upon treatment with neuraminidase or proteases. |
| T6 |
756-1042 |
Sentence |
denotes |
However, disruption of vesicles by detergents or mechanical means prior to the enzymatic treatments resulted in removal of labeled NeuAc; the extent of NeuAc removal could be correlated with that of vesicle disruption as measured by loss of latency of thiamine pyrophosphatase activity. |
| T6 |
756-1042 |
Sentence |
denotes |
However, disruption of vesicles by detergents or mechanical means prior to the enzymatic treatments resulted in removal of labeled NeuAc; the extent of NeuAc removal could be correlated with that of vesicle disruption as measured by loss of latency of thiamine pyrophosphatase activity. |
| T7 |
1043-1237 |
Sentence |
denotes |
This strongly suggests that NeuAc residues of glycoproteins are on the lumenal side of the Golgi apparatus membrane and that Golgi-derived vesicles have the same membrane orientation as in vivo. |
| T7 |
1043-1237 |
Sentence |
denotes |
This strongly suggests that NeuAc residues of glycoproteins are on the lumenal side of the Golgi apparatus membrane and that Golgi-derived vesicles have the same membrane orientation as in vivo. |
| T8 |
1238-1319 |
Sentence |
denotes |
A similar approach was used to infer a lumenal orientation of sialyltransferases. |
| T8 |
1238-1319 |
Sentence |
denotes |
A similar approach was used to infer a lumenal orientation of sialyltransferases. |
| T9 |
1320-1451 |
Sentence |
denotes |
Not all Golgi apparatus preparations routinely used may be suitable for membrane topography experiments of the kind described here. |
| T9 |
1320-1451 |
Sentence |
denotes |
Not all Golgi apparatus preparations routinely used may be suitable for membrane topography experiments of the kind described here. |