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PubMed:7298658 JSONTXT 41 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-84 Sentence denotes Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.
T1 0-84 Sentence denotes Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.
T2 85-483 Sentence denotes A sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc).
T2 85-483 Sentence denotes A sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc).
T3 484-617 Sentence denotes The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme.
T3 484-617 Sentence denotes The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme.
T4 618-723 Sentence denotes The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM.
T4 618-723 Sentence denotes The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM.
T5 724-766 Sentence denotes The reaction proceeds optimally at pH 6.2.
T5 724-766 Sentence denotes The reaction proceeds optimally at pH 6.2.
T6 767-903 Sentence denotes The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid.
T6 767-903 Sentence denotes The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid.
T7 904-1118 Sentence denotes 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel.
T7 904-1118 Sentence denotes 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel.
T8 1119-1262 Sentence denotes The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper.
T8 1119-1262 Sentence denotes The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper.
T9 1263-1372 Sentence denotes The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol.
T9 1263-1372 Sentence denotes The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol.
T10 1373-1580 Sentence denotes Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid.
T10 1373-1580 Sentence denotes Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid.
T11 1581-1750 Sentence denotes Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose.
T11 1581-1750 Sentence denotes Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose.