| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-52 |
Sentence |
denotes |
Transport of sugar nucleotides into rat liver Golgi. |
| T1 |
0-52 |
Sentence |
denotes |
Transport of sugar nucleotides into rat liver Golgi. |
| T2 |
53-81 |
Sentence |
denotes |
A new Golgi marker activity. |
| T2 |
53-81 |
Sentence |
denotes |
A new Golgi marker activity. |
| T3 |
82-414 |
Sentence |
denotes |
Following incubation of sealed, "right side out," rat liver Golgi-derived vesicles with a mixture of [3H]GDP-fucose and GDP-[14C]fucose, the difference in the 3H to 14C ratio between the incubation medium and the lumen of the vesicles was less than 11%, suggesting that the sugar nucleotide was transported intact into the vesicles. |
| T3 |
82-414 |
Sentence |
denotes |
Following incubation of sealed, "right side out," rat liver Golgi-derived vesicles with a mixture of [3H]GDP-fucose and GDP-[14C]fucose, the difference in the 3H to 14C ratio between the incubation medium and the lumen of the vesicles was less than 11%, suggesting that the sugar nucleotide was transported intact into the vesicles. |
| T4 |
415-592 |
Sentence |
denotes |
Transport of GDP-fucose weas temperature-dependent, saturable, with an apparent Km of 7.5 microM and a Vmax of 14 pmol/mg of protein/10 min and inhibited by substrate analogues. |
| T4 |
415-592 |
Sentence |
denotes |
Transport of GDP-fucose weas temperature-dependent, saturable, with an apparent Km of 7.5 microM and a Vmax of 14 pmol/mg of protein/10 min and inhibited by substrate analogues. |
| T5 |
593-754 |
Sentence |
denotes |
Pretreatment of intact Golgi vesicles with pronase inhibited transport by 80% under conditions in which sialyltransferases (a lumenal marker) were not inhibited. |
| T5 |
593-754 |
Sentence |
denotes |
Pretreatment of intact Golgi vesicles with pronase inhibited transport by 80% under conditions in which sialyltransferases (a lumenal marker) were not inhibited. |
| T6 |
755-884 |
Sentence |
denotes |
This result is consistent with a sugar nucleotide carrier protein, portions of which face the cytoplasmic side of Golgi vesicles. |
| T6 |
755-884 |
Sentence |
denotes |
This result is consistent with a sugar nucleotide carrier protein, portions of which face the cytoplasmic side of Golgi vesicles. |
| T7 |
885-1077 |
Sentence |
denotes |
Previous studies from this laboratory had shown that CMP-N-acetylneuraminic acid (CMP-NeuAc) could penetrate mouse liver microsomes in a manner suggesting carrier-mediated transport (Carey, D. |
| T7 |
885-1077 |
Sentence |
denotes |
Previous studies from this laboratory had shown that CMP-N-acetylneuraminic acid (CMP-NeuAc) could penetrate mouse liver microsomes in a manner suggesting carrier-mediated transport (Carey, D. |
| T8 |
1078-1093 |
Sentence |
denotes |
J., Sommers, L. |
| T8 |
1078-1093 |
Sentence |
denotes |
J., Sommers, L. |
| T9 |
1094-1116 |
Sentence |
denotes |
W., and Hirschberg, C. |
| T9 |
1094-1116 |
Sentence |
denotes |
W., and Hirschberg, C. |
| T10 |
1117-1145 |
Sentence |
denotes |
B. (1980) Cell 19, 597-605). |
| T10 |
1117-1145 |
Sentence |
denotes |
B. (1980) Cell 19, 597-605). |
| T11 |
1146-1301 |
Sentence |
denotes |
Subsequent to transport, a portion of the N-acetylneuraminic acid moiety became covalently linked to proteins facing the lumen of Golgi vesicles (Carey, D. |
| T11 |
1146-1301 |
Sentence |
denotes |
Subsequent to transport, a portion of the N-acetylneuraminic acid moiety became covalently linked to proteins facing the lumen of Golgi vesicles (Carey, D. |
| T12 |
1302-1324 |
Sentence |
denotes |
J., and Hirschberg, C. |
| T12 |
1302-1324 |
Sentence |
denotes |
J., and Hirschberg, C. |
| T13 |
1325-1337 |
Sentence |
denotes |
B. (1981) J. |
| T13 |
1325-1337 |
Sentence |
denotes |
B. (1981) J. |
| T14 |
1338-1343 |
Sentence |
denotes |
Biol. |
| T14 |
1338-1343 |
Sentence |
denotes |
Biol. |
| T15 |
1344-1349 |
Sentence |
denotes |
Chem. |
| T15 |
1344-1349 |
Sentence |
denotes |
Chem. |
| T16 |
1350-1364 |
Sentence |
denotes |
256, 989-993). |
| T16 |
1350-1364 |
Sentence |
denotes |
256, 989-993). |
| T17 |
1365-1593 |
Sentence |
denotes |
Upon subfractionation of rat liver into vesicles derived from the smooth and rough endoplasmic reticulum and Golgi, both the highest CMP-NeuAc specific transport activity and total transport activity were localized in the Golgi. |
| T17 |
1365-1593 |
Sentence |
denotes |
Upon subfractionation of rat liver into vesicles derived from the smooth and rough endoplasmic reticulum and Golgi, both the highest CMP-NeuAc specific transport activity and total transport activity were localized in the Golgi. |
| T18 |
1594-1745 |
Sentence |
denotes |
Any transport activity in the other vesicles could be accounted for by contamination with Golgi, as determined by established marker enzyme activities. |
| T18 |
1594-1745 |
Sentence |
denotes |
Any transport activity in the other vesicles could be accounted for by contamination with Golgi, as determined by established marker enzyme activities. |
| T19 |
1746-1932 |
Sentence |
denotes |
Transport of CMP-NeuAc was temperature-dependent, inhibited by pronase, substrate analogues, and saturable, with an apparent Km of 2.4 microM and a Vmax of 150 pmol/mg of protein/10 min. |
| T19 |
1746-1932 |
Sentence |
denotes |
Transport of CMP-NeuAc was temperature-dependent, inhibited by pronase, substrate analogues, and saturable, with an apparent Km of 2.4 microM and a Vmax of 150 pmol/mg of protein/10 min. |