| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-94 |
Sentence |
denotes |
Structure and biosynthesis of surface polymers containing polysialic acid in Escherichia coli. |
| T1 |
0-94 |
Sentence |
denotes |
Structure and biosynthesis of surface polymers containing polysialic acid in Escherichia coli. |
| T2 |
95-263 |
Sentence |
denotes |
Membranous sialyltransferase complexes from Escherichia coli K-235 catalyze the synthesis of surface polymers containing alpha-2,8-ketosidically linked polysialic acid. |
| T2 |
95-263 |
Sentence |
denotes |
Membranous sialyltransferase complexes from Escherichia coli K-235 catalyze the synthesis of surface polymers containing alpha-2,8-ketosidically linked polysialic acid. |
| T3 |
264-495 |
Sentence |
denotes |
Undecaprenyl phosphate functions as an intermediate carrier of sialic acid (NeuNAc) residues between cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc) and an endogenous acceptor (Troy, F.A., and McCloskey, M.A. (1979) J. |
| T3 |
264-495 |
Sentence |
denotes |
Undecaprenyl phosphate functions as an intermediate carrier of sialic acid (NeuNAc) residues between cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc) and an endogenous acceptor (Troy, F.A., and McCloskey, M.A. (1979) J. |
| T4 |
496-501 |
Sentence |
denotes |
Biol. |
| T4 |
496-501 |
Sentence |
denotes |
Biol. |
| T5 |
502-523 |
Sentence |
denotes |
Chem 254, 7377-7387). |
| T5 |
502-523 |
Sentence |
denotes |
Chem 254, 7377-7387). |
| T6 |
524-688 |
Sentence |
denotes |
In vitro pulse-chase experiments now confirm that polymer elongation occurs by the addition of sialyl residues to the nonreducing termini of growing nascent chains. |
| T6 |
524-688 |
Sentence |
denotes |
In vitro pulse-chase experiments now confirm that polymer elongation occurs by the addition of sialyl residues to the nonreducing termini of growing nascent chains. |
| T7 |
689-941 |
Sentence |
denotes |
Sequential periodate oxidation and borohydride reduction of radiolabeled polysialic acid was used to quantitatively convert the terminal, nonreducing sialic acid to the 7-carbon analogue, 5-acetamido-3,5-dideoxy-L-arabino-2-heptulosonic acid (NeuNAc7). |
| T7 |
689-941 |
Sentence |
denotes |
Sequential periodate oxidation and borohydride reduction of radiolabeled polysialic acid was used to quantitatively convert the terminal, nonreducing sialic acid to the 7-carbon analogue, 5-acetamido-3,5-dideoxy-L-arabino-2-heptulosonic acid (NeuNAc7). |
| T8 |
942-1105 |
Sentence |
denotes |
After complete hydrolysis of the polymers by neuraminidase, the ratio between NeuNAc and NeuNAc7 was used to determine the average degree of polymerization (D.P.). |
| T8 |
942-1105 |
Sentence |
denotes |
After complete hydrolysis of the polymers by neuraminidase, the ratio between NeuNAc and NeuNAc7 was used to determine the average degree of polymerization (D.P.). |
| T9 |
1106-1233 |
Sentence |
denotes |
The membrane preparations used as a source of enzyme contained endogenous sialyl polymers that averaged 165 residues in length. |
| T9 |
1106-1233 |
Sentence |
denotes |
The membrane preparations used as a source of enzyme contained endogenous sialyl polymers that averaged 165 residues in length. |
| T10 |
1234-1376 |
Sentence |
denotes |
During the first phase of in vitro synthesis, lasting about 90 min, 40 to 45 sialyl residues were transferred onto these endogenous acceptors. |
| T10 |
1234-1376 |
Sentence |
denotes |
During the first phase of in vitro synthesis, lasting about 90 min, 40 to 45 sialyl residues were transferred onto these endogenous acceptors. |
| T11 |
1377-1466 |
Sentence |
denotes |
Subsequent in vitro incorporation increased at a slower, constant rate for at least 16 h. |
| T11 |
1377-1466 |
Sentence |
denotes |
Subsequent in vitro incorporation increased at a slower, constant rate for at least 16 h. |
| T12 |
1467-1681 |
Sentence |
denotes |
During this second phase of synthesis, the D.P. of newly synthesized chains remained relatively constant while the number of nonreducing terminal end groups, a measure of the number of new sialyl chains, increased. |
| T12 |
1467-1681 |
Sentence |
denotes |
During this second phase of synthesis, the D.P. of newly synthesized chains remained relatively constant while the number of nonreducing terminal end groups, a measure of the number of new sialyl chains, increased. |
| T13 |
1682-1857 |
Sentence |
denotes |
These results establish that individual polymer chains are rapidly elongated in vitro to a defined length of about 200 sialyl residues, then terminated and new chains started. |
| T13 |
1682-1857 |
Sentence |
denotes |
These results establish that individual polymer chains are rapidly elongated in vitro to a defined length of about 200 sialyl residues, then terminated and new chains started. |
| T14 |
1858-2007 |
Sentence |
denotes |
The mechanism signaling chain termination, translocation of the sialyltransferase to a new acceptor, and chain reinitiation remains to be determined. |
| T14 |
1858-2007 |
Sentence |
denotes |
The mechanism signaling chain termination, translocation of the sialyltransferase to a new acceptor, and chain reinitiation remains to be determined. |
| T15 |
2008-2137 |
Sentence |
denotes |
Endogenous and enzymatically synthesized sialyl polymers were solubilized with Triton X-100 and purified to apparent homogeneity. |
| T15 |
2008-2137 |
Sentence |
denotes |
Endogenous and enzymatically synthesized sialyl polymers were solubilized with Triton X-100 and purified to apparent homogeneity. |
| T16 |
2138-2260 |
Sentence |
denotes |
Sialic acid accounted for approximately 93% of the mass of these polymers which had no free reducing terminal sialic acid. |
| T16 |
2138-2260 |
Sentence |
denotes |
Sialic acid accounted for approximately 93% of the mass of these polymers which had no free reducing terminal sialic acid. |
| T17 |
2261-2397 |
Sentence |
denotes |
This position of the molecule is presumably occupied by an as yet unidentified component which links the sialyl polymer to the membrane. |
| T17 |
2261-2397 |
Sentence |
denotes |
This position of the molecule is presumably occupied by an as yet unidentified component which links the sialyl polymer to the membrane. |