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PubMed:6795195 JSONTXT 45 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-149 Sentence denotes Purification of human midcycle cervical mucin and characterization of its oligosaccharides with respect to size, composition, and microheterogeneity.
T1 0-149 Sentence denotes Purification of human midcycle cervical mucin and characterization of its oligosaccharides with respect to size, composition, and microheterogeneity.
T2 150-341 Sentence denotes Human cervical mucin was solubilized from the gel phase of pooled midcycle cervical mucus using 6 M guanidine hydrochloride and 10 mM dithiothreitol and was then alkylated with iodoacetamide.
T2 150-341 Sentence denotes Human cervical mucin was solubilized from the gel phase of pooled midcycle cervical mucus using 6 M guanidine hydrochloride and 10 mM dithiothreitol and was then alkylated with iodoacetamide.
T3 342-456 Sentence denotes Mucin was then purified by gel filtration on Bio-Gel A-50m resin in buffer containing 0.1% sodium dodecyl sulfate.
T3 342-456 Sentence denotes Mucin was then purified by gel filtration on Bio-Gel A-50m resin in buffer containing 0.1% sodium dodecyl sulfate.
T4 457-559 Sentence denotes The purified mucin gave a single band upon electrophoresis in either 5% acrylamide or 1% agarose gels.
T4 457-559 Sentence denotes The purified mucin gave a single band upon electrophoresis in either 5% acrylamide or 1% agarose gels.
T5 560-675 Sentence denotes Protein comprised 21% of the glycoprotein by weight and amino acid analysis revealed a high content of Ser and Thr.
T5 560-675 Sentence denotes Protein comprised 21% of the glycoprotein by weight and amino acid analysis revealed a high content of Ser and Thr.
T6 676-774 Sentence denotes Saccharide analysis yielded approximate molar ratios of Fuc:Gal:GlcNAc:GalNAc:NeuAc = 1:2:1:1:0.5.
T6 676-774 Sentence denotes Saccharide analysis yielded approximate molar ratios of Fuc:Gal:GlcNAc:GalNAc:NeuAc = 1:2:1:1:0.5.
T7 775-845 Sentence denotes Inorganic sulfate, 1% by weight, was detected, but mannose was absent.
T7 775-845 Sentence denotes Inorganic sulfate, 1% by weight, was detected, but mannose was absent.
T8 846-1090 Sentence denotes Reductive alkali treatment of mucin resulted in release of oligosaccharides with concomitant conversion of 77% of GalNAc to its reduced derivative N-acetylgalactosaminitol (GalNAcol) thus demonstrating O-glycosidic linkage of GalNAc to protein.
T8 846-1090 Sentence denotes Reductive alkali treatment of mucin resulted in release of oligosaccharides with concomitant conversion of 77% of GalNAc to its reduced derivative N-acetylgalactosaminitol (GalNAcol) thus demonstrating O-glycosidic linkage of GalNAc to protein.
T9 1091-1258 Sentence denotes Reduced oligosaccharides were purified by ion exchange chromatography on DEAE-cellulose, paper chromatography, and high resolution gel filtration on Bio-Gel P-2 resin.
T9 1091-1258 Sentence denotes Reduced oligosaccharides were purified by ion exchange chromatography on DEAE-cellulose, paper chromatography, and high resolution gel filtration on Bio-Gel P-2 resin.
T10 1259-1343 Sentence denotes A total of 16 reduced oligosaccharides were identified by thin layer chromatography.
T10 1259-1343 Sentence denotes A total of 16 reduced oligosaccharides were identified by thin layer chromatography.
T11 1344-1474 Sentence denotes These included neutral, sialylated, and sulfated oligosaccharides and they varied in size from a disaccharide to a nonasaccharide.
T11 1344-1474 Sentence denotes These included neutral, sialylated, and sulfated oligosaccharides and they varied in size from a disaccharide to a nonasaccharide.
T12 1475-1720 Sentence denotes The major neutral oligosaccharide isolated (21% of recovered GalNAcol) was a tetrasaccharide, Gal:GlcNAc:GalNAcol = 2:1:1, and the major acidic oligosaccharide isolated (11% of recovered GalNAcol) was a trisaccharide, Gal:GalNAcol:NeuAc = 1:1:1.
T12 1475-1720 Sentence denotes The major neutral oligosaccharide isolated (21% of recovered GalNAcol) was a tetrasaccharide, Gal:GlcNAc:GalNAcol = 2:1:1, and the major acidic oligosaccharide isolated (11% of recovered GalNAcol) was a trisaccharide, Gal:GalNAcol:NeuAc = 1:1:1.