| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-149 |
Sentence |
denotes |
Purification of human midcycle cervical mucin and characterization of its oligosaccharides with respect to size, composition, and microheterogeneity. |
| T1 |
0-149 |
Sentence |
denotes |
Purification of human midcycle cervical mucin and characterization of its oligosaccharides with respect to size, composition, and microheterogeneity. |
| T2 |
150-341 |
Sentence |
denotes |
Human cervical mucin was solubilized from the gel phase of pooled midcycle cervical mucus using 6 M guanidine hydrochloride and 10 mM dithiothreitol and was then alkylated with iodoacetamide. |
| T2 |
150-341 |
Sentence |
denotes |
Human cervical mucin was solubilized from the gel phase of pooled midcycle cervical mucus using 6 M guanidine hydrochloride and 10 mM dithiothreitol and was then alkylated with iodoacetamide. |
| T3 |
342-456 |
Sentence |
denotes |
Mucin was then purified by gel filtration on Bio-Gel A-50m resin in buffer containing 0.1% sodium dodecyl sulfate. |
| T3 |
342-456 |
Sentence |
denotes |
Mucin was then purified by gel filtration on Bio-Gel A-50m resin in buffer containing 0.1% sodium dodecyl sulfate. |
| T4 |
457-559 |
Sentence |
denotes |
The purified mucin gave a single band upon electrophoresis in either 5% acrylamide or 1% agarose gels. |
| T4 |
457-559 |
Sentence |
denotes |
The purified mucin gave a single band upon electrophoresis in either 5% acrylamide or 1% agarose gels. |
| T5 |
560-675 |
Sentence |
denotes |
Protein comprised 21% of the glycoprotein by weight and amino acid analysis revealed a high content of Ser and Thr. |
| T5 |
560-675 |
Sentence |
denotes |
Protein comprised 21% of the glycoprotein by weight and amino acid analysis revealed a high content of Ser and Thr. |
| T6 |
676-774 |
Sentence |
denotes |
Saccharide analysis yielded approximate molar ratios of Fuc:Gal:GlcNAc:GalNAc:NeuAc = 1:2:1:1:0.5. |
| T6 |
676-774 |
Sentence |
denotes |
Saccharide analysis yielded approximate molar ratios of Fuc:Gal:GlcNAc:GalNAc:NeuAc = 1:2:1:1:0.5. |
| T7 |
775-845 |
Sentence |
denotes |
Inorganic sulfate, 1% by weight, was detected, but mannose was absent. |
| T7 |
775-845 |
Sentence |
denotes |
Inorganic sulfate, 1% by weight, was detected, but mannose was absent. |
| T8 |
846-1090 |
Sentence |
denotes |
Reductive alkali treatment of mucin resulted in release of oligosaccharides with concomitant conversion of 77% of GalNAc to its reduced derivative N-acetylgalactosaminitol (GalNAcol) thus demonstrating O-glycosidic linkage of GalNAc to protein. |
| T8 |
846-1090 |
Sentence |
denotes |
Reductive alkali treatment of mucin resulted in release of oligosaccharides with concomitant conversion of 77% of GalNAc to its reduced derivative N-acetylgalactosaminitol (GalNAcol) thus demonstrating O-glycosidic linkage of GalNAc to protein. |
| T9 |
1091-1258 |
Sentence |
denotes |
Reduced oligosaccharides were purified by ion exchange chromatography on DEAE-cellulose, paper chromatography, and high resolution gel filtration on Bio-Gel P-2 resin. |
| T9 |
1091-1258 |
Sentence |
denotes |
Reduced oligosaccharides were purified by ion exchange chromatography on DEAE-cellulose, paper chromatography, and high resolution gel filtration on Bio-Gel P-2 resin. |
| T10 |
1259-1343 |
Sentence |
denotes |
A total of 16 reduced oligosaccharides were identified by thin layer chromatography. |
| T10 |
1259-1343 |
Sentence |
denotes |
A total of 16 reduced oligosaccharides were identified by thin layer chromatography. |
| T11 |
1344-1474 |
Sentence |
denotes |
These included neutral, sialylated, and sulfated oligosaccharides and they varied in size from a disaccharide to a nonasaccharide. |
| T11 |
1344-1474 |
Sentence |
denotes |
These included neutral, sialylated, and sulfated oligosaccharides and they varied in size from a disaccharide to a nonasaccharide. |
| T12 |
1475-1720 |
Sentence |
denotes |
The major neutral oligosaccharide isolated (21% of recovered GalNAcol) was a tetrasaccharide, Gal:GlcNAc:GalNAcol = 2:1:1, and the major acidic oligosaccharide isolated (11% of recovered GalNAcol) was a trisaccharide, Gal:GalNAcol:NeuAc = 1:1:1. |
| T12 |
1475-1720 |
Sentence |
denotes |
The major neutral oligosaccharide isolated (21% of recovered GalNAcol) was a tetrasaccharide, Gal:GlcNAc:GalNAcol = 2:1:1, and the major acidic oligosaccharide isolated (11% of recovered GalNAcol) was a trisaccharide, Gal:GalNAcol:NeuAc = 1:1:1. |