| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-82 |
Sentence |
denotes |
Peptide mapping of the human transferrin receptor in normal and transformed cells. |
| T1 |
0-82 |
Sentence |
denotes |
Peptide mapping of the human transferrin receptor in normal and transformed cells. |
| T2 |
83-422 |
Sentence |
denotes |
Human transferrin receptor protein from various human cell lines in tissue culture (phytohemagglutinin-activated lymphocytes, normal embryonic fibroblasts (WI-38), a choriocarcinoma (Be Wo), an epidermoid carcinoma (KB), and a metastatic breast carcinoma (MCF-7)) was metabolically labeled with [35S]methionine and purified by two methods. |
| T2 |
83-422 |
Sentence |
denotes |
Human transferrin receptor protein from various human cell lines in tissue culture (phytohemagglutinin-activated lymphocytes, normal embryonic fibroblasts (WI-38), a choriocarcinoma (Be Wo), an epidermoid carcinoma (KB), and a metastatic breast carcinoma (MCF-7)) was metabolically labeled with [35S]methionine and purified by two methods. |
| T3 |
423-546 |
Sentence |
denotes |
The first method involves affinity chromatography of the transferrin receptor with a transferrin-linked Sepharose 4B resin. |
| T3 |
423-546 |
Sentence |
denotes |
The first method involves affinity chromatography of the transferrin receptor with a transferrin-linked Sepharose 4B resin. |
| T4 |
547-703 |
Sentence |
denotes |
The second method involves direct immunoprecipitation of the receptor protein with a goat polyclonal antibody raised against placental transferrin receptor. |
| T4 |
547-703 |
Sentence |
denotes |
The second method involves direct immunoprecipitation of the receptor protein with a goat polyclonal antibody raised against placental transferrin receptor. |
| T5 |
704-875 |
Sentence |
denotes |
Additionally, transferrin receptor from normal gestational placenta was similarly radiolabeled with [35S]methionine and subsequently purified with transferrin-bound resin. |
| T5 |
704-875 |
Sentence |
denotes |
Additionally, transferrin receptor from normal gestational placenta was similarly radiolabeled with [35S]methionine and subsequently purified with transferrin-bound resin. |
| T6 |
876-1081 |
Sentence |
denotes |
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reduced receptor isolated by both purification techniques revealed an Mr = 94,000 protein to be present in all cells assayed for this study. |
| T6 |
876-1081 |
Sentence |
denotes |
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reduced receptor isolated by both purification techniques revealed an Mr = 94,000 protein to be present in all cells assayed for this study. |
| T7 |
1082-1383 |
Sentence |
denotes |
Partial proteolytic digestion with Staphylococcus aureus protease followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed identical mapping patterns of the Mr = 94,000 protein isolated from each cell line, irrespective of the isolation technique employed to purify the receptor. |
| T7 |
1082-1383 |
Sentence |
denotes |
Partial proteolytic digestion with Staphylococcus aureus protease followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed identical mapping patterns of the Mr = 94,000 protein isolated from each cell line, irrespective of the isolation technique employed to purify the receptor. |
| T8 |
1384-1685 |
Sentence |
denotes |
These findings indicate an identical peptide structure of the human transferrin receptor in cells with a differentiated function for iron metabolism, in normal cells undergoing mitosis, and in neoplastic cells in long term culture, within the limits of detectability of the proteolytic digestion maps. |
| T8 |
1384-1685 |
Sentence |
denotes |
These findings indicate an identical peptide structure of the human transferrin receptor in cells with a differentiated function for iron metabolism, in normal cells undergoing mitosis, and in neoplastic cells in long term culture, within the limits of detectability of the proteolytic digestion maps. |