| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-124 |
Sentence |
denotes |
Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter. |
| T1 |
0-124 |
Sentence |
denotes |
Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter. |
| T2 |
125-127 |
Sentence |
denotes |
I. |
| T2 |
125-127 |
Sentence |
denotes |
I. |
| T3 |
128-165 |
Sentence |
denotes |
Cloning and characterization studies. |
| T3 |
128-165 |
Sentence |
denotes |
Cloning and characterization studies. |
| T4 |
166-338 |
Sentence |
denotes |
To assess the role of neighboring DNA sequences in gene regulation, poly(dA).poly(dT) and poly(dG).poly(dC) were cloned adjacent to promoters of the lactose control region. |
| T4 |
166-338 |
Sentence |
denotes |
To assess the role of neighboring DNA sequences in gene regulation, poly(dA).poly(dT) and poly(dG).poly(dC) were cloned adjacent to promoters of the lactose control region. |
| T5 |
339-680 |
Sentence |
denotes |
Recombinant plasmids were constructed which were suitable for large scale purification of restriction fragments containing these promoters, 95-base pair (bp) AluI fragments containing the lack operator and promoter for the lac wild type and for the catabolite gene activating the protein-independent mutant, lac UV5, were cloned into pBR322. |
| T5 |
339-680 |
Sentence |
denotes |
Recombinant plasmids were constructed which were suitable for large scale purification of restriction fragments containing these promoters, 95-base pair (bp) AluI fragments containing the lack operator and promoter for the lac wild type and for the catabolite gene activating the protein-independent mutant, lac UV5, were cloned into pBR322. |
| T6 |
681-799 |
Sentence |
denotes |
Homopolymers of varying lengths were inserted into the -60 region of these promoters using recombinant DNA techniques. |
| T6 |
681-799 |
Sentence |
denotes |
Homopolymers of varying lengths were inserted into the -60 region of these promoters using recombinant DNA techniques. |
| T7 |
800-1084 |
Sentence |
denotes |
Six of the recombinant plasmids were chosen for detailed analysis: wild type (wt); wt-AT, containing 70 bp of poly(dA).poly(dT); wt-GC, containing 23 bp of poly(dG).poly(dC); UV5; UV5-AT, containing 70 bp of poly(dA).poly(dT) and finally UV5-GC, containing 43 bp of poly(dG).poly(dC). |
| T7 |
800-1084 |
Sentence |
denotes |
Six of the recombinant plasmids were chosen for detailed analysis: wild type (wt); wt-AT, containing 70 bp of poly(dA).poly(dT); wt-GC, containing 23 bp of poly(dG).poly(dC); UV5; UV5-AT, containing 70 bp of poly(dA).poly(dT) and finally UV5-GC, containing 43 bp of poly(dG).poly(dC). |
| T8 |
1085-1161 |
Sentence |
denotes |
These plasmids were characterized by restriction mapping and DNA sequencing. |
| T8 |
1085-1161 |
Sentence |
denotes |
These plasmids were characterized by restriction mapping and DNA sequencing. |
| T9 |
1162-1324 |
Sentence |
denotes |
The effects of the DNA homopolymers on the interaction of the Escherichia coli RNA polymerase with the promoters were studied using nitrocellulose filter binding. |
| T9 |
1162-1324 |
Sentence |
denotes |
The effects of the DNA homopolymers on the interaction of the Escherichia coli RNA polymerase with the promoters were studied using nitrocellulose filter binding. |
| T10 |
1325-1463 |
Sentence |
denotes |
The results show that poly(dA).poly(dT) increases the level of RNA polymerase binding, whereas poly(dG).poly(dC) has no detectable effect. |
| T10 |
1325-1463 |
Sentence |
denotes |
The results show that poly(dA).poly(dT) increases the level of RNA polymerase binding, whereas poly(dG).poly(dC) has no detectable effect. |