| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-108 |
Sentence |
denotes |
Functional inactivation of lac alpha-peptide mRNA by a factor that purifies that Escherichia coli RNase III. |
| T1 |
0-108 |
Sentence |
denotes |
Functional inactivation of lac alpha-peptide mRNA by a factor that purifies that Escherichia coli RNase III. |
| T2 |
109-262 |
Sentence |
denotes |
Using RNA-directed synthesis of the alpha-peptide of beta-galactosidase as an assay, a factor was purified that inactivated further function of the mRNA. |
| T2 |
109-262 |
Sentence |
denotes |
Using RNA-directed synthesis of the alpha-peptide of beta-galactosidase as an assay, a factor was purified that inactivated further function of the mRNA. |
| T3 |
263-426 |
Sentence |
denotes |
In the presence of Ca2+ ions to inhibit most nuclease activity, inactivation of mRNA occurred during incubation with ribosomes or with a 1 M KCl wash of ribosomes. |
| T3 |
263-426 |
Sentence |
denotes |
In the presence of Ca2+ ions to inhibit most nuclease activity, inactivation of mRNA occurred during incubation with ribosomes or with a 1 M KCl wash of ribosomes. |
| T4 |
427-584 |
Sentence |
denotes |
The inactivation activity required Mg2+ ions, and purified as a single factor which did not bind to DEAE-cellulose, but bound reversibly to phosphocellulose. |
| T4 |
427-584 |
Sentence |
denotes |
The inactivation activity required Mg2+ ions, and purified as a single factor which did not bind to DEAE-cellulose, but bound reversibly to phosphocellulose. |
| T5 |
585-673 |
Sentence |
denotes |
The factor eluted from Sephadex G-150 with an apparent molecular weight of about 43,000. |
| T5 |
585-673 |
Sentence |
denotes |
The factor eluted from Sephadex G-150 with an apparent molecular weight of about 43,000. |
| T6 |
674-921 |
Sentence |
denotes |
Purified 700-fold, it showed no detectable exonuclease activity, and little or no cleavage of a variety of single-stranded substrates, including full length lac operon mRNA; but repurified inactivated mRNA was still inactive for protein synthesis. |
| T6 |
674-921 |
Sentence |
denotes |
Purified 700-fold, it showed no detectable exonuclease activity, and little or no cleavage of a variety of single-stranded substrates, including full length lac operon mRNA; but repurified inactivated mRNA was still inactive for protein synthesis. |
| T7 |
922-994 |
Sentence |
denotes |
The factor did not inhibit poly(U)-directed polyphenylalanine synthesis. |
| T7 |
922-994 |
Sentence |
denotes |
The factor did not inhibit poly(U)-directed polyphenylalanine synthesis. |
| T8 |
995-1175 |
Sentence |
denotes |
When proteins isolated from the ribosomal wash were individually tested, highly purified RNase III, which purifies in the same way and has the same size, also inactivated lac mRNA. |
| T8 |
995-1175 |
Sentence |
denotes |
When proteins isolated from the ribosomal wash were individually tested, highly purified RNase III, which purifies in the same way and has the same size, also inactivated lac mRNA. |
| T9 |
1176-1303 |
Sentence |
denotes |
The ribosomal wash from an RNase III- strain showed little if any activity compared to that from an isogenic RNase III+ strain. |
| T9 |
1176-1303 |
Sentence |
denotes |
The ribosomal wash from an RNase III- strain showed little if any activity compared to that from an isogenic RNase III+ strain. |
| T10 |
1304-1418 |
Sentence |
denotes |
The possibility of a site-specific inactivating cleavage of mRNA by RNase III at or near the 5' end is considered. |
| T10 |
1304-1418 |
Sentence |
denotes |
The possibility of a site-specific inactivating cleavage of mRNA by RNase III at or near the 5' end is considered. |