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PubMed:4066704 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-97 Sentence denotes Phosphorylation of histidine in proteins by a nuclear extract of Physarum polycephalum plasmodia.
T1 0-97 Sentence denotes Phosphorylation of histidine in proteins by a nuclear extract of Physarum polycephalum plasmodia.
T2 98-276 Sentence denotes A high salt nuclear extract from the true slime mold Physarum polycephalum was used as a source of kinase activity for the incubation of calf thymus histones with [gamma-32P]ATP.
T2 98-276 Sentence denotes A high salt nuclear extract from the true slime mold Physarum polycephalum was used as a source of kinase activity for the incubation of calf thymus histones with [gamma-32P]ATP.
T3 277-368 Sentence denotes A major proportion of the 32P incorporated into histones was acid-labile and alkali-stable.
T3 277-368 Sentence denotes A major proportion of the 32P incorporated into histones was acid-labile and alkali-stable.
T4 369-590 Sentence denotes The nature of the alkali-stable phosphorylated component was analyzed by subjecting the phosphorylated protein to total alkaline hydrolysis and separating the resultant phosphoamino acids by anion exchange chromatography.
T4 369-590 Sentence denotes The nature of the alkali-stable phosphorylated component was analyzed by subjecting the phosphorylated protein to total alkaline hydrolysis and separating the resultant phosphoamino acids by anion exchange chromatography.
T5 591-763 Sentence denotes The 32P-labeled material co-chromatographed with phosphohistidine standards and did not co-chromatograph with phosphoserine, phosphothreonine, or phosphotyrosine standards.
T5 591-763 Sentence denotes The 32P-labeled material co-chromatographed with phosphohistidine standards and did not co-chromatograph with phosphoserine, phosphothreonine, or phosphotyrosine standards.
T6 764-1141 Sentence denotes In similar experiments using reversed phase high-performance liquid chromatography to separate the phosphoamino acids, the 32P-labeled phosphoamino acid behaved like the 1-isomer of phosphohistidine, in not being retained by the column, and unlike 3-phosphohistidine, phosphoserine, phosphothreonine, phosphotyrosine, and phosphoarginine, which were all retained on the column.
T6 764-1141 Sentence denotes In similar experiments using reversed phase high-performance liquid chromatography to separate the phosphoamino acids, the 32P-labeled phosphoamino acid behaved like the 1-isomer of phosphohistidine, in not being retained by the column, and unlike 3-phosphohistidine, phosphoserine, phosphothreonine, phosphotyrosine, and phosphoarginine, which were all retained on the column.
T7 1142-1329 Sentence denotes Histone H4 was a good substrate for the histidine kinase activity and the location of the phosphorylated histidine residue was probed by peptide mapping using chymotrypsin or V8 protease.
T7 1142-1329 Sentence denotes Histone H4 was a good substrate for the histidine kinase activity and the location of the phosphorylated histidine residue was probed by peptide mapping using chymotrypsin or V8 protease.
T8 1330-1433 Sentence denotes Both maps were consistent with labeling of histidine 75 and inconsistent with labeling of histidine 18.
T8 1330-1433 Sentence denotes Both maps were consistent with labeling of histidine 75 and inconsistent with labeling of histidine 18.
T9 1434-1533 Sentence denotes The data show that Physarum nuclei contain a major kinase activity which produces phosphohistidine.
T9 1434-1533 Sentence denotes The data show that Physarum nuclei contain a major kinase activity which produces phosphohistidine.
T10 1534-1779 Sentence denotes The methods we have developed for studying this kinase activity provide the basis for a complete characterization of the structure and function of the Physarum enzyme and can be applied to the study of similar kinase activities in other systems.
T10 1534-1779 Sentence denotes The methods we have developed for studying this kinase activity provide the basis for a complete characterization of the structure and function of the Physarum enzyme and can be applied to the study of similar kinase activities in other systems.