| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-50 |
Sentence |
denotes |
Characterization of ovarian gonadotropin receptor. |
| T1 |
0-50 |
Sentence |
denotes |
Characterization of ovarian gonadotropin receptor. |
| T2 |
51-95 |
Sentence |
denotes |
Monomer and associated form of the receptor. |
| T2 |
51-95 |
Sentence |
denotes |
Monomer and associated form of the receptor. |
| T3 |
96-282 |
Sentence |
denotes |
We have purified luteinizing hormone/human choriogonadotropin (hCG) receptor from rat ovary by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose chromatography. |
| T3 |
96-282 |
Sentence |
denotes |
We have purified luteinizing hormone/human choriogonadotropin (hCG) receptor from rat ovary by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose chromatography. |
| T4 |
283-458 |
Sentence |
denotes |
The purified receptor, previously identified as a single protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Kusuda, S., and Dufau, M.L. (1986) J. |
| T4 |
283-458 |
Sentence |
denotes |
The purified receptor, previously identified as a single protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Kusuda, S., and Dufau, M.L. (1986) J. |
| T5 |
459-464 |
Sentence |
denotes |
Biol. |
| T5 |
459-464 |
Sentence |
denotes |
Biol. |
| T6 |
465-470 |
Sentence |
denotes |
Chem. |
| T6 |
465-470 |
Sentence |
denotes |
Chem. |
| T7 |
471-653 |
Sentence |
denotes |
261, 16161-16168), was further characterized by radioiodination with 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycouril, and column chromatography on wheat germ lectin-Sepharose. |
| T7 |
471-653 |
Sentence |
denotes |
261, 16161-16168), was further characterized by radioiodination with 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycouril, and column chromatography on wheat germ lectin-Sepharose. |
| T8 |
654-766 |
Sentence |
denotes |
Autoradiography of SDS-PAGE analysis under reducing conditions showed a single radiolabeled band of Mr = 80,000. |
| T8 |
654-766 |
Sentence |
denotes |
Autoradiography of SDS-PAGE analysis under reducing conditions showed a single radiolabeled band of Mr = 80,000. |
| T9 |
767-857 |
Sentence |
denotes |
The radioiodinated receptors treated with peptide:N-glycosidase F migrated at Mr = 54,000. |
| T9 |
767-857 |
Sentence |
denotes |
The radioiodinated receptors treated with peptide:N-glycosidase F migrated at Mr = 54,000. |
| T10 |
858-1056 |
Sentence |
denotes |
Treatment with neuraminidase alone caused only a minor reduction in molecular weight, and subsequent treatment with endo-alpha-N-acetyl-D-galactosaminidase had little further effect on the receptor. |
| T10 |
858-1056 |
Sentence |
denotes |
Treatment with neuraminidase alone caused only a minor reduction in molecular weight, and subsequent treatment with endo-alpha-N-acetyl-D-galactosaminidase had little further effect on the receptor. |
| T11 |
1057-1206 |
Sentence |
denotes |
When the radioiodinated receptor was analyzed by fast protein liquid chromatography, a single broad peak was eluted with Mr of approximately 350,000. |
| T11 |
1057-1206 |
Sentence |
denotes |
When the radioiodinated receptor was analyzed by fast protein liquid chromatography, a single broad peak was eluted with Mr of approximately 350,000. |
| T12 |
1207-1360 |
Sentence |
denotes |
The higher Mr of radioiodinated receptors than that of native receptors (Mr = 190,000 dimeric form) could be due to the aggregation of labeled molecules. |
| T12 |
1207-1360 |
Sentence |
denotes |
The higher Mr of radioiodinated receptors than that of native receptors (Mr = 190,000 dimeric form) could be due to the aggregation of labeled molecules. |
| T13 |
1361-1436 |
Sentence |
denotes |
These complexes dissociated into the monomeric form in the presence of SDS. |
| T13 |
1361-1436 |
Sentence |
denotes |
These complexes dissociated into the monomeric form in the presence of SDS. |
| T14 |
1437-1616 |
Sentence |
denotes |
To determine whether the monomers can bind hormone, the purified unlabeled receptors resolved with SDS were electroblotted to nitrocellulose membranes and incubated with 125I-hCG. |
| T14 |
1437-1616 |
Sentence |
denotes |
To determine whether the monomers can bind hormone, the purified unlabeled receptors resolved with SDS were electroblotted to nitrocellulose membranes and incubated with 125I-hCG. |
| T15 |
1617-1735 |
Sentence |
denotes |
Autoradiograms of the blots showed a band of monomer (Mr = 78,000) as well as one of dimer (Mr approximately 150,000). |
| T15 |
1617-1735 |
Sentence |
denotes |
Autoradiograms of the blots showed a band of monomer (Mr = 78,000) as well as one of dimer (Mr approximately 150,000). |
| T16 |
1736-1976 |
Sentence |
denotes |
These studies have demonstrated that the luteinizing hormone/hCG receptors are predominantly N-linked glycosylated and suggest that the native receptor is a dimer of identical hormone binding subunits associated by noncovalent interactions. |
| T16 |
1736-1976 |
Sentence |
denotes |
These studies have demonstrated that the luteinizing hormone/hCG receptors are predominantly N-linked glycosylated and suggest that the native receptor is a dimer of identical hormone binding subunits associated by noncovalent interactions. |
| T17 |
1977-2105 |
Sentence |
denotes |
Although the individual subunits can bind hormone, it is conceivable that the dimeric form is necessary for signal transduction. |
| T17 |
1977-2105 |
Sentence |
denotes |
Although the individual subunits can bind hormone, it is conceivable that the dimeric form is necessary for signal transduction. |