| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-156 |
Sentence |
denotes |
Comprehensive Analysis of the Proteome and PTMomes of C2C12 Myoblasts Reveals that Sialylation Plays a Role in the Differentiation of Skeletal Muscle Cells. |
| T2 |
157-274 |
Sentence |
denotes |
The C2C12 myoblast is a model that has been used extensively to study the process of skeletal muscle differentiation. |
| T3 |
275-406 |
Sentence |
denotes |
Proteomics has advanced our understanding of skeletal muscle biology and also the differentiation process of skeletal muscle cells. |
| T4 |
407-591 |
Sentence |
denotes |
However, there is still no comprehensive analysis of C2C12 myoblast proteomes, which is important for the understanding of key drivers for the differentiation of skeletal muscle cells. |
| T5 |
592-744 |
Sentence |
denotes |
Here, we conducted multidimensional proteome profiling to get a comprehensive analysis of proteomes and PTMomes of C2C12 myoblasts with a TiSH strategy. |
| T6 |
745-923 |
Sentence |
denotes |
A total of 8313 protein groups were identified, including 7827 protein groups from nonmodified peptides, 3803 phosphoproteins, and 977 formerly sialylated N-linked glycoproteins. |
| T7 |
924-1101 |
Sentence |
denotes |
Integrated analysis of proteomic and PTMomic data showed that almost all of the kinases and transcription factors in the muscle cell differentiation pathway were phosphorylated. |
| T8 |
1102-1206 |
Sentence |
denotes |
Further analysis indicated that sialylation might play a role in the differentiation of C2C12 myoblasts. |
| T9 |
1207-1353 |
Sentence |
denotes |
Further functional analysis demonstrated that C2C12 myoblasts showed a decreased level of sialylation during skeletal muscle cell differentiation. |
| T10 |
1354-1504 |
Sentence |
denotes |
Inhibition of sialylation with the sialyltransferase inhibitor 3Fax-Neu5Ac resulted in the lower expression of MHC and suppression of myoblast fusion. |
| T11 |
1505-1615 |
Sentence |
denotes |
In all, these results indicate that sialylation has an effect on the differentiation of skeletal muscle cells. |
