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PubMed:32675285 JSONTXT 39 Projects

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Id Subject Object Predicate Lexical cue
T1 0-94 Sentence denotes Intracellular autoactivation of TMPRSS11A, an airway epithelial transmembrane serine protease.
T2 95-211 Sentence denotes Type II transmembrane serine proteases (TTSPs) are a group of enzymes participating in diverse biological processes.
T3 212-278 Sentence denotes Some members of the TTSP family are implicated in viral infection.
T4 279-521 Sentence denotes TMPRSS11A is a TTSP expressed on the surface of airway epithelial cells, which has been shown to cleave and activate spike proteins of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome coronaviruses (CoVs).
T5 522-669 Sentence denotes In this study, we examined the mechanism underlying the activation cleavage of TMPRSS11A that converts the one-chain zymogen to a two-chain enzyme.
T6 670-912 Sentence denotes By expression in human embryonic kidney 293, esophageal EC9706, and lung epithelial A549 and 16HBE cells, Western blotting, and site-directed mutagenesis, we found that the activation cleavage of human TMPRSS11A was mediated by autocatalysis.
T7 913-1270 Sentence denotes Moreover, we found that TMPRSS11A activation cleavage occurred before the protein reached the cell surface, as indicated by studies with trypsin digestion to remove cell surface proteins, treatment with cell organelle-disturbing agents to block intracellular protein trafficking, and analysis of a soluble form of TMPRSS11A without the transmembrane domain.
T8 1271-1349 Sentence denotes We also showed that TMPRSS11A was able to cleave the SARS-CoV-2 spike protein.
T9 1350-1524 Sentence denotes These results reveal an intracellular autocleavage mechanism in TMPRSS11A zymogen activation, which differs from the extracellular zymogen activation reported in other TTSPs.
T10 1525-1619 Sentence denotes These findings provide new insights into the diverse mechanisms in regulating TTSP activation.