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PubMed:32094225 JSONTXT 37 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-131 Sentence denotes The antiviral compound remdesivir potently inhibits RNA-dependent RNA polymerase from Middle East respiratory syndrome coronavirus.
T2 132-369 Sentence denotes Antiviral drugs for managing infections with human coronaviruses are not yet approved, posing a serious challenge to current global efforts aimed at containing the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
T3 370-552 Sentence denotes Remdesivir (RDV) is an investigational compound with a broad spectrum of antiviral activities against RNA viruses, including SARS-CoV and Middle East respiratory syndrome (MERS-CoV).
T4 553-631 Sentence denotes RDV is a nucleotide analog inhibitor of RNA-dependent RNA polymerases (RdRps).
T5 632-826 Sentence denotes Here, we co-expressed the MERS-CoV nonstructural proteins nsp5, nsp7, nsp8, and nsp12 (RdRp) in insect cells as a part a polyprotein to study the mechanism of inhibition of MERS-CoV RdRp by RDV.
T6 827-896 Sentence denotes We initially demonstrated that nsp8 and nsp12 form an active complex.
T7 897-987 Sentence denotes The triphosphate form of the inhibitor (RDV-TP) competes with its natural counterpart ATP.
T8 988-1167 Sentence denotes Of note, the selectivity value for RDV-TP obtained here with a steady-state approach suggests that it is more efficiently incorporated than ATP and two other nucleotide analogues.
T9 1168-1259 Sentence denotes Once incorporated at position i, the inhibitor caused RNA synthesis arrest at position i+3.
T10 1260-1331 Sentence denotes Hence, the likely mechanism of action is delayed RNA chain termination.
T11 1332-1445 Sentence denotes The additional three nucleotides may protect the inhibitor from excision by the viral 3'-5' exonuclease activity.
T12 1446-1551 Sentence denotes Together, these results help to explain the high potency of RDV against RNA viruses in cell-based assays.