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PubMed:31416836 JSONTXT 36 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-120 Sentence denotes Ega3 from the fungal pathogen Aspergillus fumigatus is an endo-α-1,4-galactosaminidase that disrupts microbial biofilms.
T1 0-120 Sentence denotes Ega3 from the fungal pathogen Aspergillus fumigatus is an endo-α-1,4-galactosaminidase that disrupts microbial biofilms.
T2 121-234 Sentence denotes Aspergillus fumigatus is an opportunistic fungal pathogen that causes both chronic and acute invasive infections.
T2 121-234 Sentence denotes Aspergillus fumigatus is an opportunistic fungal pathogen that causes both chronic and acute invasive infections.
T3 235-350 Sentence denotes Galactosaminogalactan (GAG) is an integral component of the A. fumigatus biofilm matrix and a key virulence factor.
T3 235-350 Sentence denotes Galactosaminogalactan (GAG) is an integral component of the A. fumigatus biofilm matrix and a key virulence factor.
T4 351-483 Sentence denotes GAG is a heterogeneous linear α-1,4-linked exopolysaccharide of galactose and GalNAc that is partially deacetylated after secretion.
T4 351-483 Sentence denotes GAG is a heterogeneous linear α-1,4-linked exopolysaccharide of galactose and GalNAc that is partially deacetylated after secretion.
T5 484-574 Sentence denotes A cluster of five co-expressed genes has been linked to GAG biosynthesis and modification.
T5 484-574 Sentence denotes A cluster of five co-expressed genes has been linked to GAG biosynthesis and modification.
T6 575-719 Sentence denotes One gene in this cluster, ega3, is annotated as encoding a putative α-1,4-galactosaminidase belonging to glycoside hydrolase family 114 (GH114).
T6 575-719 Sentence denotes One gene in this cluster, ega3, is annotated as encoding a putative α-1,4-galactosaminidase belonging to glycoside hydrolase family 114 (GH114).
T7 720-925 Sentence denotes Herein, we show that recombinant Ega3 is an active glycoside hydrolase that disrupts GAG-dependent A. fumigatus and Pel polysaccharide-dependent Pseudomonas aeruginosa biofilms at nanomolar concentrations.
T7 720-925 Sentence denotes Herein, we show that recombinant Ega3 is an active glycoside hydrolase that disrupts GAG-dependent A. fumigatus and Pel polysaccharide-dependent Pseudomonas aeruginosa biofilms at nanomolar concentrations.
T8 926-1102 Sentence denotes Using MS and functional assays, we demonstrate that Ega3 is an endo-acting α-1,4-galactosaminidase whose activity depends on the conserved acidic residues, Asp-189 and Glu-247.
T8 926-1102 Sentence denotes Using MS and functional assays, we demonstrate that Ega3 is an endo-acting α-1,4-galactosaminidase whose activity depends on the conserved acidic residues, Asp-189 and Glu-247.
T9 1103-1349 Sentence denotes X-ray crystallographic structural analysis of the apo Ega3 and an Ega3-galactosamine complex, at 1.76 and 2.09 Å resolutions, revealed a modified (β/α)8-fold with a deep electronegative cleft, which upon ligand binding is capped to form a tunnel.
T9 1103-1349 Sentence denotes X-ray crystallographic structural analysis of the apo Ega3 and an Ega3-galactosamine complex, at 1.76 and 2.09 Å resolutions, revealed a modified (β/α)8-fold with a deep electronegative cleft, which upon ligand binding is capped to form a tunnel.
T10 1350-1540 Sentence denotes Our structural analysis coupled with in silico docking studies also uncovered the molecular determinants for galactosamine specificity and substrate binding at the -2 to +1 binding subsites.
T10 1350-1540 Sentence denotes Our structural analysis coupled with in silico docking studies also uncovered the molecular determinants for galactosamine specificity and substrate binding at the -2 to +1 binding subsites.
T11 1541-1769 Sentence denotes The findings in this study increase the structural and mechanistic understanding of the GH114 family, which has >600 members encoded by plant and opportunistic human pathogens, as well as in industrially used bacteria and fungi.
T11 1541-1769 Sentence denotes The findings in this study increase the structural and mechanistic understanding of the GH114 family, which has >600 members encoded by plant and opportunistic human pathogens, as well as in industrially used bacteria and fungi.